Validated Rp-Hplc Method For Estimation Of Chrysin In Bulk Form And Nanostructured Lipid Carriers For Topical Application

Abstract

Chrysin, a flavonoid, occurs naturally in plants and possesses many pharmacological actions, but there is a lack of suitable analytical methods for its estimation. To develop a simple analytical method and validate it for the estimation of chrysin using reverse phase high-performance liquid chromatography (RP-HPLC). To develop a simple analytical method and validate it for estimation of Chrysin using reverse phase high performance liquid chromatography (RP-HPLC). Isocratic elution was carried out in methanol and 0.1% v/v formic acid in a 70:30 ratio using a C-18 reverse-phase column. The flow rate was set to 1 mL min-1 and the detection wavelength at 268 nm. As per ICH Q2 (R1) guidelines, the developed method was validated in terms of accuracy, precision, system suitability, and robustness. Isocratic elution was carried out in methanol and 0.1% v/v formic acid in 70:30 ratio using C-18 reverse-phase column. The flow rate was set to 1 mL min-1 and detection wavelength at 268 nm. As per ICH Q2 (R1) guidelines, the developed method was validated in terms of accuracy, precision, system suitability and robustness. The retention time of Chrysin was found at 10.269 min. In the concentration range of 2-10 g/mL, the developed method was linear with a regression coefficient (R2) value of 0.998. The mean percentage recovery of chrysin was found within 95-105% at all three levels, which confirms that the developed method was accurate. Moreover, the % RSD was found to be less than 2% confirming that the developed method was precise. The limit of detection and limit of quantification were found to be 0.071 and 0.217 µg/mL. Moreover, the validated method was robust with no significant changes in response to variation in flow rate, mobile phase composition, wavelength, and different lots of columns. It was concluded that the developed method has passed all validation tests and was successfully applied to estimate the presence of Chrysin in bulk as well as in pharmaceutical formulations. It was concluded that the developed method has passed all the validation tests and successfully applied to estimate the presence of Chrysin in bulk as well as in pharmaceutical formulations.