Anticancer activity of ethanol extract, n-hexane, and the ethyl acetate fraction of tin leaves (Ficus carica l.) on MCF-7 breast cancer cell lines

Aji Winanta, Widhi Yana Sari
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Abstract

Background: Breast cancer is ranked as the second-highest cause of death in Indonesia. Many of the available cancer treatments result in severe adverse effects for patients. Tin (Ficus carica L.) leaves contain ingredients that can function as anticancer agents. Objective: The study aims to identify phytochemical secondary metabolites in tin leaves and the cytotoxic activity of the extract and tin leaf fraction on MCF-7 cells. Method: Tin leaf powder was macerated with 70% ethanol for 7 days. The extracts were fractionated using n-hexane and ethyl acetate. An identification test with ethanol extract was then carried out using spray reagents. The cytotoxic activity of ethanolic extract, n-hexane and ethyl acetate fraction of tin leaf was determined using the MTT method. Result: The results of the identification with TLC found that the ethanol extract of tin leaves contains flavonoids and steroids. The ethyl acetate fraction of tin leaf was thought to have weak cytotoxic properties with an IC50 value of 274.5877 ug/mL while the ethanol extract and n-heksan fraction of tin leaf do not have cytotoxic properties because they have an IC50 value of 562.827 and 576.3552 ug/mL. Conclusion: Based on the results, tin leaves have the potency to be developed as a chemopreventive agent for MCF-7 breast cancer cells. Keywords: Breast Cancer, MCF-7 cells, tin leaf (Ficus carica), cytotoxic activity
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锡叶乙醇提取物、正己烷和乙酸乙酯部分对MCF-7乳腺癌细胞株的抗癌作用
背景:在印度尼西亚,乳腺癌被列为第二大死因。许多现有的癌症治疗方法会对患者产生严重的不良影响。锡(Ficus carica L.)的叶子含有抗癌成分。目的:研究锡叶中植物化学次生代谢产物及其提取物和锡叶提取物对MCF-7细胞的细胞毒活性。方法:用70%乙醇浸泡锡叶粉7 d。提取液采用正己烷和乙酸乙酯进行分馏。然后用喷雾试剂对乙醇提取物进行鉴定试验。采用MTT法测定了锡叶乙醇提取物、正己烷和乙酸乙酯部分的细胞毒活性。结果:薄层色谱鉴别发现锡叶乙醇提取物中含有黄酮类化合物和甾类化合物。锡叶的乙酸乙酯部分的IC50值为274.5877 ug/mL,而锡叶的乙醇提取物和n-heksan部分的IC50值分别为562.827和576.3552 ug/mL,不具有细胞毒性。结论:锡叶具有开发作为MCF-7乳腺癌细胞化学预防剂的潜力。关键词:乳腺癌,MCF-7细胞,锡叶,细胞毒活性
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Pengetahuan, sikap, dan perilaku konsumsi suplemen dan obat tradisional mahasiswa Universitas Jember saat pandemi COVID-19 Hubungan Tingkat Pengetahuan dengan Perilaku Swamedikasi Obat Golongan NSAID di Apotek X Banjarmasin EFEKTIVITAS KOMBINASI EKSTRAK ETANOL 70% DAUN RAMANIA (Bouea macrophylla Griffith) dan GLIBENKLAMID DENGAN METODE INTOLERANSI GLUKOSA PERBANDINGAN KADAR GLUKOSA DARAH DAN HBA1C PADA INSULIN GLARGINE DAN DETEMIR PASIEN DIABETES MELITUS TYPE 2 DI RUMAH SAKIT UMUM DAERAH ULIN BANJARMASIN The study of conformity of the pharmaceutical service in Puskesmas Bogor City towards PMK No. 74, 2016
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