{"title":"cDNA Cloning and Sequence Analysis of Genome Segment S10 from Rice Black-Streaked Dwarf Virus","authors":"Zhang Heng-mu","doi":"10.1631/jzus.2002.0216","DOIUrl":null,"url":null,"abstract":"Genome segment S10 of a Zhejiang isolate of rice black-streaked dwarf virus (RBSDV-Zj) was amplified by RT-PCR and sequenced. It consisted of 1801 nt (EMBL accession number is AJ297433) with only one open reading frame which encodes a polypeptide with molecular weight of 63.1 kD. Sequence comparison showed 93.8% and 96.2% homology at level of nucleotide and amino acid sequences between RBSDV-Zj and Japanese isolate (RBSDV-Jap), respectively;and shared 87.1% and 92.0% homology at level of nucleotide and acid sequences between RBSDV-Zj and Italian MRDV, respectively.","PeriodicalId":10160,"journal":{"name":"Chinese Journal of Rice Science","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chinese Journal of Rice Science","FirstCategoryId":"1091","ListUrlMain":"https://doi.org/10.1631/jzus.2002.0216","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 1
Abstract
Genome segment S10 of a Zhejiang isolate of rice black-streaked dwarf virus (RBSDV-Zj) was amplified by RT-PCR and sequenced. It consisted of 1801 nt (EMBL accession number is AJ297433) with only one open reading frame which encodes a polypeptide with molecular weight of 63.1 kD. Sequence comparison showed 93.8% and 96.2% homology at level of nucleotide and amino acid sequences between RBSDV-Zj and Japanese isolate (RBSDV-Jap), respectively;and shared 87.1% and 92.0% homology at level of nucleotide and acid sequences between RBSDV-Zj and Italian MRDV, respectively.
期刊介绍：
Chinese Journal of Rice Science（CJRS）(ISSN 1001-7216), a bi-monthly journal published by China National Rice Research Institute, mainly publishes original research papers in fields of fundamental and applied fundamental scientific researches on rice, including genetics and breeding, germplasm resources, agronomy, bioengineering, plant protection, soil and plant nutrition, plant physiology, cereal chemistry and processing, agricultural economics and paddy field development, and agricultural engineering, etc. The journal also publishes reviews and experimental techniques. Articles are in Chinese with English abstract.
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