Genome segment S10 of a Zhejiang isolate of rice black-streaked dwarf virus (RBSDV-Zj) was amplified by RT-PCR and sequenced. It consisted of 1801 nt (EMBL accession number is AJ297433) with only one open reading frame which encodes a polypeptide with molecular weight of 63.1 kD. Sequence comparison showed 93.8% and 96.2% homology at level of nucleotide and amino acid sequences between RBSDV-Zj and Japanese isolate (RBSDV-Jap), respectively;and shared 87.1% and 92.0% homology at level of nucleotide and acid sequences between RBSDV-Zj and Italian MRDV, respectively.
{"title":"cDNA Cloning and Sequence Analysis of Genome Segment S10 from Rice Black-Streaked Dwarf Virus","authors":"Zhang Heng-mu","doi":"10.1631/jzus.2002.0216","DOIUrl":"https://doi.org/10.1631/jzus.2002.0216","url":null,"abstract":"Genome segment S10 of a Zhejiang isolate of rice black-streaked dwarf virus (RBSDV-Zj) was amplified by RT-PCR and sequenced. It consisted of 1801 nt (EMBL accession number is AJ297433) with only one open reading frame which encodes a polypeptide with molecular weight of 63.1 kD. Sequence comparison showed 93.8% and 96.2% homology at level of nucleotide and amino acid sequences between RBSDV-Zj and Japanese isolate (RBSDV-Jap), respectively;and shared 87.1% and 92.0% homology at level of nucleotide and acid sequences between RBSDV-Zj and Italian MRDV, respectively.","PeriodicalId":10160,"journal":{"name":"Chinese Journal of Rice Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81332271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1990-01-01DOI: 10.1142/9789812814272_0031
D. Ling, C. Liang, Z. Ma, M. F. Chen, B. He
In past five years from 1984 to 1988, 48 cases of male sterile (ms) mutants in nine varieties were found, in which 30 cases belonged to R_1 generation and 28 to R_2 generation. The frequency of ms variation in R, generation was around 1% and 2% in R_2 generatiom. According to the characters of ms-mutants and their genetic expressions,pollen grain free(nuclear sterile) and abortive pollen (cytoplasmic genetic sterile) could be classified, In 1R54, two cases of pollen grain free ms-mutants were discovered similar in different generations (R_1 and R_2 generation) and different years (1985 and 1986) . Among ms-mutants of the abortive pollen type, one (54257) was found to be the cytoplasmic genetic male sterile mutant. Zheng-Shan 97, Er-Jiu-Ai,162-5 (a stable somaclonefrom IR52) and Gui-Chao 2 could maintain male sterility when crossed to the ms line (54257) . IR24, IR36, IR54,Shuang-Er-Zhan and others could restore its fertility. This result showed that "54257" was a cytoplasmic ms-mutant.This is the first sample that the cytoplasmic ms mutant could be induced by tissue culture in the world.
{"title":"Somaclonal Male-Sterile Mutants and Their Expressions in Indica Rice","authors":"D. Ling, C. Liang, Z. Ma, M. F. Chen, B. He","doi":"10.1142/9789812814272_0031","DOIUrl":"https://doi.org/10.1142/9789812814272_0031","url":null,"abstract":"In past five years from 1984 to 1988, 48 cases of male sterile (ms) mutants in nine varieties were found, in which 30 cases belonged to R_1 generation and 28 to R_2 generation. The frequency of ms variation in R, generation was around 1% and 2% in R_2 generatiom. According to the characters of ms-mutants and their genetic expressions,pollen grain free(nuclear sterile) and abortive pollen (cytoplasmic genetic sterile) could be classified, In 1R54, two cases of pollen grain free ms-mutants were discovered similar in different generations (R_1 and R_2 generation) and different years (1985 and 1986) . Among ms-mutants of the abortive pollen type, one (54257) was found to be the cytoplasmic genetic male sterile mutant. Zheng-Shan 97, Er-Jiu-Ai,162-5 (a stable somaclonefrom IR52) and Gui-Chao 2 could maintain male sterility when crossed to the ms line (54257) . IR24, IR36, IR54,Shuang-Er-Zhan and others could restore its fertility. This result showed that \"54257\" was a cytoplasmic ms-mutant.This is the first sample that the cytoplasmic ms mutant could be induced by tissue culture in the world.","PeriodicalId":10160,"journal":{"name":"Chinese Journal of Rice Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88505487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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