Nano-biosensor base on protected glucose oxidase nanoparticles

Abstract

Covalent modification of redox enzyme-glucose oxidase (GOx)-within porous composite organic/inorganic network was described. The polymerization of organic/inorganic network was synthesized via a three-step process, which consisted of a covalent modification of GOx followed by a polymerization between modified enzyme and methacryloxypropyltrimethoxysilane (MPS) and then hydrolysis and crosslinking. The synthesized GOx nanoparticles were less than 20 nm in size. They were observed by transmission electron microscope (TEM) and analyzed using Fourier transform infrared spectrophotometer (FT-IR). The nano-biosensor based on protected GOx nanoparticles demonstrated extension of primarily lifetime and detection of extremely limited concentration (pM) in human serum. The hybrid organic/inorganic network was determined by application of protected glucose oxidase nanoparticles in electrochemical nano-biosensor. The hybrid enzyme nanostructures were expected as a method to stabilize enzyme for other biocatalytic application.