Health Status of Spiny Lobster Panulirus homarus with Sub-Mersible Net Cage System in the Different Depths at Kepulauan Seribu, DKI Jakarta

D. Wahjuningrum, I. Effendi, Y. Hadiroseyani, T. Budiardi, I. Diatin, M. Setiawati, Yuni Pujiastuti, Agus Oman Sudrajat, Yonvitner, Sri Nuryati, Putri Utami
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Abstract

ABSTRACT Cultivation of Panulirus homarus lobster is now carried out with sub-mersible net cage system at a certain depth in order to obtain optimal temperature, light and water pressure. The purpose of this study was to evaluate the health status of the sand lobster P. homarus which was kept in sub-mersible net cage system measuring 250 cm × 272 cm × 135 cm with a depth of 6 m and 8 m in the waters of Semak Daun Island, Seribu Islands, DKI Jakarta. The average size of lobster seeds used was 93.23 ± 0.99 g/head with a density of 4 lobsters/m2. Lobsters were fed trash fish, molluscs and crustaceans, with a frequency of twice a day at 07.00 WIB 30% and 17.00 WIB 70% of the lobster biomass weight. This study used a completely randomized design with the two depth treatments mentioned above and three replications. Observations of total haemocyte count, differential haemocyte count, phenoloxidase activity, respiratory burst phagocytic activity and histology of lobster hepatopancreas were performed twice every 14 days. Based on the above observations, the depth does not affect the immune response, there is no visible damage to the cells and tissues of the lobster hepatopancreas. Keywords: haemolymph, histology, lobster cultivation, sea, sub-mersible net cage system
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雅加达Kepulauan Seribu不同深度潜式网笼系统对小龙虾健康状况的影响
摘要为了获得最佳的温度、光照和水压,目前采用一定深度的潜式网箱系统进行小龙虾养殖。本研究旨在评价在印尼首都雅加达塞里布群岛斯迈克顿岛水域养殖的250 cm × 272 cm × 135 cm、水深6 m和8 m的水下网箱系统中沙龙虾的健康状况。龙虾种子平均用量为93.23±0.99 g/头,密度为4只/m2。投喂垃圾鱼、软体动物和甲壳类,投喂频率为每天2次,投喂量分别为龙虾生物量重量的30%和70%。本研究采用完全随机设计,采用上述两种深度处理,3个重复。每14 d观察2次龙虾肝胰腺总血细胞计数、差异血细胞计数、酚氧化酶活性、呼吸爆发吞噬活性及组织学变化。根据上述观察,深度不影响免疫反应,对龙虾肝胰腺的细胞和组织没有明显的损伤。关键词:血淋巴,组织学,龙虾养殖,海洋,潜网笼系统
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