Pub Date : 2024-07-24DOI: 10.19027/jai.23.2.176-188
Sukma Sari Anggraeni, Sukenda, Sri Nuryati, Dinamella Wahjuningrum
Vibrio parahaemolyticus adalah salah satu bakteri patogen yang menyebabkan kematian pada udang vaname Litopenaeus vannamei. Penelitian ini bertujuan untuk menganalisis distribusi V. parahaemolyticus pada tubuh udang melalui perendaman serta kerusakan yang ditimbulkan melalui pengamatan gejala klinis, angka lempeng total bakteri dan polymerase chain reaction serta analisis histopatologi. Penelitian terdiri atas dua perlakuan yaitu udang terinfeksi dan udang kontrol tanpa infeksi dengan pengulangan 4 kali. Udang diinfeksi melalui perendaman bakteri V. parahaemolyticus 107 CFU/mL selama 30 menit dan kemudian udang dipindah pada wadah pemeliharaan. Mortalitas udang diamati hingga 24 jam pascainfeksi. Pengambilan sampel organ insang, hepatopankreas, usus dan hemolim udang dilakukan pada waktu 6, 12, dan 24 jam pascainfeksi. Berdasarkan pengamatan udang pascainfeksi 24 jam menunjukkan tingkat mortalitas hingga 97,08% dan tidak ada kematian pada udang kontrol. Kelimpahan V. parahaemolyticus pada organ insang, hepatopankreas, dan usus udang berkisar antara 104-106 CFU/g. Udang yang terinfeksi V. parahaemolyticus memiliki gejala klinis seperti warna tubuh dan hepatopankreas pucat serta usus yang kosong. Pengamatan histopatologi menunjukkan bahwa organ insang, hepatopankreas, dan usus udang yang terinfeksi mengalami derajat kerusakan ringan, sedang, hingga parah. Hasil uji konfirmasi kematian udang menggunakan PCR menunjukkan hasil positif disebabkan oleh V. parahaemolyticus. Pengamatan respons imun udang seperti total hemosit aktivitas fagositik, phenoloxidase dan respiratory burst menujukkan hasil yang berbeda nyata (P<0,05) antara udang yang terinfeksi dan kontrol tanpa infeksi V. parahaemolyticus. Disimpulkan bahwa infeksi V. parahaemolyticus melalui perendaman sebagai model infeksi alami terdistribusi pada organ insang, hepatopankreas, dan usus udang mulai jam ke-6 hingga jam ke-24 mulai dari 104-106 CFU/g. Kata kunci: distribusi, Vibrio parahaemolyticus, perendaman, Litopenaeus vannamei
{"title":"Distribusi Vibrio parahaemolyticus pada Udang Vaname Litopenaeus vannamei melalui Perendaman sebagai Model Infeksi Alami","authors":"Sukma Sari Anggraeni, Sukenda, Sri Nuryati, Dinamella Wahjuningrum","doi":"10.19027/jai.23.2.176-188","DOIUrl":"https://doi.org/10.19027/jai.23.2.176-188","url":null,"abstract":"Vibrio parahaemolyticus adalah salah satu bakteri patogen yang menyebabkan kematian pada udang vaname Litopenaeus vannamei. Penelitian ini bertujuan untuk menganalisis distribusi V. parahaemolyticus pada tubuh udang melalui perendaman serta kerusakan yang ditimbulkan melalui pengamatan gejala klinis, angka lempeng total bakteri dan polymerase chain reaction serta analisis histopatologi. Penelitian terdiri atas dua perlakuan yaitu udang terinfeksi dan udang kontrol tanpa infeksi dengan pengulangan 4 kali. Udang diinfeksi melalui perendaman bakteri V. parahaemolyticus 107 CFU/mL selama 30 menit dan kemudian udang dipindah pada wadah pemeliharaan. Mortalitas udang diamati hingga 24 jam pascainfeksi. Pengambilan sampel organ insang, hepatopankreas, usus dan hemolim udang dilakukan pada waktu 6, 12, dan 24 jam pascainfeksi. Berdasarkan pengamatan udang pascainfeksi 24 jam menunjukkan tingkat mortalitas hingga 97,08% dan tidak ada kematian pada udang kontrol. Kelimpahan V. parahaemolyticus pada organ insang, hepatopankreas, dan usus udang berkisar antara 104-106 CFU/g. Udang yang terinfeksi V. parahaemolyticus memiliki gejala klinis seperti warna tubuh dan hepatopankreas pucat serta usus yang kosong. Pengamatan histopatologi menunjukkan bahwa organ insang, hepatopankreas, dan usus udang yang terinfeksi mengalami derajat kerusakan ringan, sedang, hingga parah. Hasil uji konfirmasi kematian udang menggunakan PCR menunjukkan hasil positif disebabkan oleh V. parahaemolyticus. Pengamatan respons imun udang seperti total hemosit aktivitas fagositik, phenoloxidase dan respiratory burst menujukkan hasil yang berbeda nyata (P<0,05) antara udang yang terinfeksi dan kontrol tanpa infeksi V. parahaemolyticus. Disimpulkan bahwa infeksi V. parahaemolyticus melalui perendaman sebagai model infeksi alami terdistribusi pada organ insang, hepatopankreas, dan usus udang mulai jam ke-6 hingga jam ke-24 mulai dari 104-106 CFU/g. \u0000 \u0000Kata kunci: distribusi, Vibrio parahaemolyticus, perendaman, Litopenaeus vannamei","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141808259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-23DOI: 10.19027/jai.23.2.168-175
S. N. F. Zakaria, Nur Hazwani, Cheng-Ann Chen
The aim of the present study was to determine the extenders such as calcium-free saline, Ringer’s solution and phosphate buffer and to determine the effect of different concentrations of cryoprotectants with different duration of exposure for short term storage of mud crab, Scylla tranquebarica sperm cells. A total of 30 mud crabs were used in the present study. In the present study, three treatments with three replicates were tested to determine the suitable extender. Besides, three treatments (dimethyl sulfoxide, glycine and glycerol) were tested to determine the suitable cryoprotectants. Eight treatments (25, 20, 16, 4, 2, -4, -20 and -80 °C) were assessed to determine the optimal cooling rate. The result showed the phosphate buffer solution produced the highest sperm viability (82.36 ± 5.84 %) after 60 min compared the Calcium free saline and Ringer’s solution. The results for sperm viability at -4 °C, -20 C and -80 °C after 24 hours were 95.63 0.13 % (5 % glycine), 91.51 ± 1.91 % (5 % DMSO) and 93.97 ± 0.43 % (5 % glycine). By using the 5 % glycine as the best cryoprotectant, the results for the sperm viability in 6 h, 12 h, 24 h, day 7, day 14 and day 21 at 80 °C were 95.17 ± 0.92 %, 94.11 ± 0.64 %, 93.31 ± 0.51 %, 90.84 ± 2.05 % and 82.82 ± 0.41 %. In a nutshell, the phosphate buffer and 5 % glycine were the best extender and cryoprotectant in the present study.
{"title":"Effect of different extenders and cryoprotectants on sperm viability of mud crab, Scylla tranquebarica for short term storage","authors":"S. N. F. Zakaria, Nur Hazwani, Cheng-Ann Chen","doi":"10.19027/jai.23.2.168-175","DOIUrl":"https://doi.org/10.19027/jai.23.2.168-175","url":null,"abstract":"The aim of the present study was to determine the extenders such as calcium-free saline, Ringer’s solution and phosphate buffer and to determine the effect of different concentrations of cryoprotectants with different duration of exposure for short term storage of mud crab, Scylla tranquebarica sperm cells. A total of 30 mud crabs were used in the present study. In the present study, three treatments with three replicates were tested to determine the suitable extender. Besides, three treatments (dimethyl sulfoxide, glycine and glycerol) were tested to determine the suitable cryoprotectants. Eight treatments (25, 20, 16, 4, 2, -4, -20 and -80 °C) were assessed to determine the optimal cooling rate. The result showed the phosphate buffer solution produced the highest sperm viability (82.36 ± 5.84 %) after 60 min compared the Calcium free saline and Ringer’s solution. The results for sperm viability at -4 °C, -20 C and -80 °C after 24 hours were 95.63 0.13 % (5 % glycine), 91.51 ± 1.91 % (5 % DMSO) and 93.97 ± 0.43 % (5 % glycine). By using the 5 % glycine as the best cryoprotectant, the results for the sperm viability in 6 h, 12 h, 24 h, day 7, day 14 and day 21 at 80 °C were 95.17 ± 0.92 %, 94.11 ± 0.64 %, 93.31 ± 0.51 %, 90.84 ± 2.05 % and 82.82 ± 0.41 %. In a nutshell, the phosphate buffer and 5 % glycine were the best extender and cryoprotectant in the present study. \u0000 ","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"3 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141810952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-12DOI: 10.19027/jai.23.2.147-156
E. I. Romadhona, Novi Megawati, Handang Widantara, Sutanti Sutanti, Arif Rahman Ardiansyah, Kiki Mariya Dewi, Annisa Fitri Larassagita, Aditia Firman, Moh Risky, Rahmat Rahmat, Ayub Ayub
Caridina boehmei is an ornamental shrimp native to Sulawesi that has been successfully cultured and marketed in Indonesia. However, molecular information about this shrimp is not yet available. This study aimed to characterize cytochrome c oxidase subunit I (CO1) and small subunit ribosomal RNA (18S rDNA) genes. Shrimp samples were extracted, and DNA was amplified using CO1 and 18S rDNA primers. The sequencing results were then analyzed by the neighbor-joining method. The 654 bp partial coding sequence (cds) of the CO1 and 380 bp partial cds of 18S rDNA have been successfully amplified from genomic DNA. The CO1 sequencing produced fragments consisting of 25.84% adenine, 20.95% cytosine, 19.11% guanine, and 34.10% thymine. The 18S rDNA sequencing produced fragments consisting of 24.47% adenine, 26.05% cytosine, 28.16% guanine, and 21.32% thymine. Phylogenetic analysis based on the CO1 gene revealed that C. boehmei was grouped with C. variabilis (MK190014), C. brachydactyla (MK190011), and Caridina sp. (MK190012). Phylogenetic analysis based on the 18S rDNA gene revealed that C. boehmei was identical to Caridina sp. Suphan Buri-1 MF622000, C. serratirostris KP725708, C. multidentata JF346236, C. formosae GQ131924, and Caridina sp. Suphan Buri-2 MF622001. The lowest-highest pairwise distance based on CO1 and 18S rDNA was consecutively 0.0017-0.2247 (0.17-22.47%) and 0.0000-0.1218 (0-12.18%). Keywords: 18S rDNA, Caridina boehmei, CO1, DNA barcoding, ornamental shrimp ABSTRAK Caridina boehmei merupakan udang hias asli Sulawesi yang telah berhasil dibudidayakan dan dipasarkan di Indonesia. Kendati demikian, informasi molekuler mengenai udang ini belum tersedia. Tujuan penelitian ini yaitu melakukan karakterisasi menggunakan gen sitokrom c oksidase subunit I (CO1) dan RNA ribosomal subunit kecil (18S rDNA). Sampel udang diekstraksi kemudian DNA diamplifikasi dengan menggunakan primer CO1 dan 18S rDNA. Hasil sekuensing kemudian dianalisis dengan metode neighbor-joining. Sekuen koding (coding sequence, cds) parsial 654 bp dari CO1 dan cds parsial 380 bp dari gen 18S rDNA) telah berhasil diamplifikasi dari DNA genom. Sekuensing CO1 menghasilkan fragmen yang terdiri dari 25,84% adenin, 20,95% sitosin, 19,11% guanin, dan 34,10% timin, sedangkan 18S rDNA menghasilkan fragmen yang terdiri dari 24,47% adenin, 26,05% sitosin, 28,16% guanin, dan 21,32% timin. Analisis filogenetik berdasarkan gen CO1 menunjukkan bahwa C. boehmei sekelompok dengan C. variabilis (MK190014), C. brachydactyla (MK190011), dan Caridina sp. (MK190012). Analisis filogenetik berdasarkan gen 18S rDNA menunjukkan bahwa C. boehmei identik dengan Caridina sp. Suphan Buri-1 MF622000, C. serratirostris KP725708, C. multidentata JF346236, C. formosae GQ131924, and Caridina sp. Suphan Buri-2 MF622001. Jarak berpasangan terendah hingga tertinggi berdasarkan CO1 dan 18S rDNA berturut-turut adalah 0,0017-0,2247 (0,17-22,47%) dan 0,0000-0,1218 (0-12,18%). Kata kunci: 18S rDNA, Caridina
Caridina boehmei 是一种原产于苏拉威西岛的观赏虾，已在印度尼西亚成功养殖和销售。然而，有关这种虾的分子信息尚未获得。本研究旨在确定细胞色素 c 氧化酶亚单位 I（CO1）和小亚单位核糖体 RNA（18S rDNA）基因的特征。提取对虾样本，使用 CO1 和 18S rDNA 引物扩增 DNA。然后用邻接法对测序结果进行分析。从基因组 DNA 中成功扩增出 CO1 的 654 bp 部分编码序列（cds）和 18S rDNA 的 380 bp 部分 cds。CO1 测序产生的片段由 25.84% 腺嘌呤、20.95% 胞嘧啶、19.11% 鸟嘌呤和 34.10% 胸腺嘧啶组成。18S rDNA 测序产生的片段包括 24.47% 腺嘌呤、26.05% 胞嘧啶、28.16% 鸟嘌呤和 21.32% 胸腺嘧啶。基于 CO1 基因的系统进化分析表明，C. boehmei 与 C. variabilis (MK190014)、C. brachydactyla (MK190011) 和 Caridina sp. (MK190012)归为一类。基于 18S rDNA 基因的系统发生分析表明，C. boehmei 与 Caridina sp.基于CO1和18S rDNA的最低-最高配对距离连续为0.0017-0.2247（0.17-22.47%）和0.0000-0.1218（0-12.18%）。 关键词18S rDNA Boehmei Caridina CO1 DNA 条形码 观赏虾 ABSTRAK Caridina boehmei merupakan udang hias asli Sulawesi yang telah berhasil dibudidayakan dan dipasarkan di Indonesia.从目前的情况来看，有关苏拉威西地区的信息已经过时。该研究的目的是了解基因 I 氧化物酶亚基（CO1）和 RNA 核糖体亚基 kecil（18S rDNA）。通过使用 CO1 和 18S rDNA 引物对 DNA 进行扩增。通过相邻连接方法对样本进行分析。编码序列（cds）与 CO1 的比对长度为 654 bp，而与 18S rDNA 基因的比对长度为 380 bp。CO1 的片段占 25.84%的腺嘌呤、20.95%的西托辛、19.11%的鸟嘌呤和 34.10%的蒂敏，而 18S rDNA 的片段占 24.47%的腺嘌呤、26.05%的西托辛、28.16%的鸟嘌呤和 21.32%的蒂敏。基因 CO1 的丝状基因组分析表明，C. boehmei sekelompok dengan C. variabilis（MK190014）、C. brachydactyla（MK190011）和 Caridina sp.（MK190012）。Analisis filogenetik berdasarkan gen 18S rDNA menunjukkan bahwa C. boehmei identik dengan Caridina sp. Suphan Buri-1 MF622000, C. serratirostris KP725708, C. multidentata JF346236, C. formosae GQ131924, and Caridina sp. Suphan Buri-2 MF622001.CO1 和 18S rDNA 的比对结果分别为 0,0017-0,2247 (0,17-22,47%) 和 0,0000-0,1218 (0-12,18%)。 Kata kunci: 18S rDNA, Caridina boehmei, CO1, DNA barcode, udang hias
{"title":"Characterization of CO1 and 18S rDNA Gene from The Domesticated Indonesian Native Ornamental Shrimp Caridina boehmei","authors":"E. I. Romadhona, Novi Megawati, Handang Widantara, Sutanti Sutanti, Arif Rahman Ardiansyah, Kiki Mariya Dewi, Annisa Fitri Larassagita, Aditia Firman, Moh Risky, Rahmat Rahmat, Ayub Ayub","doi":"10.19027/jai.23.2.147-156","DOIUrl":"https://doi.org/10.19027/jai.23.2.147-156","url":null,"abstract":"Caridina boehmei is an ornamental shrimp native to Sulawesi that has been successfully cultured and marketed in Indonesia. However, molecular information about this shrimp is not yet available. This study aimed to characterize cytochrome c oxidase subunit I (CO1) and small subunit ribosomal RNA (18S rDNA) genes. Shrimp samples were extracted, and DNA was amplified using CO1 and 18S rDNA primers. The sequencing results were then analyzed by the neighbor-joining method. The 654 bp partial coding sequence (cds) of the CO1 and 380 bp partial cds of 18S rDNA have been successfully amplified from genomic DNA. The CO1 sequencing produced fragments consisting of 25.84% adenine, 20.95% cytosine, 19.11% guanine, and 34.10% thymine. The 18S rDNA sequencing produced fragments consisting of 24.47% adenine, 26.05% cytosine, 28.16% guanine, and 21.32% thymine. Phylogenetic analysis based on the CO1 gene revealed that C. boehmei was grouped with C. variabilis (MK190014), C. brachydactyla (MK190011), and Caridina sp. (MK190012). Phylogenetic analysis based on the 18S rDNA gene revealed that C. boehmei was identical to Caridina sp. Suphan Buri-1 MF622000, C. serratirostris KP725708, C. multidentata JF346236, C. formosae GQ131924, and Caridina sp. Suphan Buri-2 MF622001. The lowest-highest pairwise distance based on CO1 and 18S rDNA was consecutively 0.0017-0.2247 (0.17-22.47%) and 0.0000-0.1218 (0-12.18%). \u0000 \u0000Keywords: 18S rDNA, Caridina boehmei, CO1, DNA barcoding, ornamental shrimp \u0000 \u0000ABSTRAK \u0000 \u0000Caridina boehmei merupakan udang hias asli Sulawesi yang telah berhasil dibudidayakan dan dipasarkan di Indonesia. Kendati demikian, informasi molekuler mengenai udang ini belum tersedia. Tujuan penelitian ini yaitu melakukan karakterisasi menggunakan gen sitokrom c oksidase subunit I (CO1) dan RNA ribosomal subunit kecil (18S rDNA). Sampel udang diekstraksi kemudian DNA diamplifikasi dengan menggunakan primer CO1 dan 18S rDNA. Hasil sekuensing kemudian dianalisis dengan metode neighbor-joining. Sekuen koding (coding sequence, cds) parsial 654 bp dari CO1 dan cds parsial 380 bp dari gen 18S rDNA) telah berhasil diamplifikasi dari DNA genom. Sekuensing CO1 menghasilkan fragmen yang terdiri dari 25,84% adenin, 20,95% sitosin, 19,11% guanin, dan 34,10% timin, sedangkan 18S rDNA menghasilkan fragmen yang terdiri dari 24,47% adenin, 26,05% sitosin, 28,16% guanin, dan 21,32% timin. Analisis filogenetik berdasarkan gen CO1 menunjukkan bahwa C. boehmei sekelompok dengan C. variabilis (MK190014), C. brachydactyla (MK190011), dan Caridina sp. (MK190012). Analisis filogenetik berdasarkan gen 18S rDNA menunjukkan bahwa C. boehmei identik dengan Caridina sp. Suphan Buri-1 MF622000, C. serratirostris KP725708, C. multidentata JF346236, C. formosae GQ131924, and Caridina sp. Suphan Buri-2 MF622001. Jarak berpasangan terendah hingga tertinggi berdasarkan CO1 dan 18S rDNA berturut-turut adalah 0,0017-0,2247 (0,17-22,47%) dan 0,0000-0,1218 (0-12,18%). \u0000 \u0000Kata kunci: 18S rDNA, Caridina","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"62 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141653168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-12DOI: 10.19027/jai.23.2.157-167
Adam Saba Anggara, Ida Ayu Mirah Meliana Dewi, Kismono Kudoasmoro, Putu Eka Sudaryatma, Putu Angga Wiradana
Infectious disease is a major challenge in fish and shrimp farming systems caused by microorganisms including bacteria, viruses and parasites. Physical detection has limitations in detecting diseases in cultivated animals earlier, because of the varied clinical symptoms. The aims of this study was to identify the physical and molecular presence of infectious pathogens in fish and shrimp cultivated in several ponds in West Java, Banten and Jakarta. Monitoring was carried out in two stages, namely the period March–April 2021 and September 2021 by taking samples from several ponds in Bogor, Tangerang, Depok and Jakarta. The sample criteria used were fish and shrimp showing normal and clinical symptoms of being attacked by a disease which were collected by purposive sampling. The collected samples were examined in two stages, namely physical examination of clinical symptoms and molecular examination using PCR. The results showed that of the 24 species samples collected, 17 species showed normal clinical symptoms and seven species showed clinical symptoms. Of the 24 fish and shrimp samples, four samples were infected (2 samples from normal species and 2 samples with clinical symptoms). The infected normal samples were Osphronemus goramy and Litopenaeus vannamei. The infected samples with clinical symptoms were Carassius auratus by Aeromonas salmonicida and red zebra cichlid (Metriaclima estherae) by Red Sea Bream Iridoviral Disease (RSBIV). Physical testing supported by molecular detection of aquatic animals can be an effort to manage aquaculture systems in Indonesia. Keyword: aquaculture, emerging disease, fisheries products, molecular assay. ABSTRAK Penyakit infeksi adalah tantangan utama pada sistem budidaya ikan dan udang yang disebabkan oleh mikroorganisme termasuk bakteri, virus, dan parasit. Deteksi secara fisik memiliki keterbatasan dalam mengetahui lebih dini penyakit pada hewan budidaya, karena gejala klinis yang bervariasi. Tujuan dari penelitian ini adalah untuk melakukan identifikasi fisik dan molekuler keberadaan patogen infeksi pada ikan dan udang yang dibudidayakan di beberapa pertambakan di Jawa Barat, Banten, dan Jakarta. Pemantauan dilakukan dalam dua tahap yaitu periode Maret – April 2021 dan September 2021 dengan mengambil sampel dari beberapa pertambakan di Bogor, Tangerang, Depok, dan Jakarta. Kriteria sampel yang digunakan adalah ikan dan udang yang menunjukkan gejala klinis terserang oleh penyakit yang dikumpulkan secara purposive sampling. Sampel yang terkumpul diperiksa dalam dua tahap yaitu pemeriksaan fisik gejala klinis dan pemeriksaan molekuler menggunakan PCR. Hasil menunjukkan dari 24 sampel spesies yang terkumpul, sebanyak 17 spesies menunjukkan gejala klinis normal dan tujuh spesies menunjukkan gejala klinis. Dari 24 sampel ikan dan udang, terdapat empat sampel yang terinfeksi (2 sampel dari spesies normal dan 2 sampel dengan gejala klinis). Sampel normal yang terinfeksi adalah Osphronemus goramy dan Lito
{"title":"Physical and Molecular Examinations of Fisheries Products Associated with Bacterial, Virological, and Parasitical Disease Collected From Several Area in West Java and Jakarta, Indonesia","authors":"Adam Saba Anggara, Ida Ayu Mirah Meliana Dewi, Kismono Kudoasmoro, Putu Eka Sudaryatma, Putu Angga Wiradana","doi":"10.19027/jai.23.2.157-167","DOIUrl":"https://doi.org/10.19027/jai.23.2.157-167","url":null,"abstract":"Infectious disease is a major challenge in fish and shrimp farming systems caused by microorganisms including bacteria, viruses and parasites. Physical detection has limitations in detecting diseases in cultivated animals earlier, because of the varied clinical symptoms. The aims of this study was to identify the physical and molecular presence of infectious pathogens in fish and shrimp cultivated in several ponds in West Java, Banten and Jakarta. Monitoring was carried out in two stages, namely the period March–April 2021 and September 2021 by taking samples from several ponds in Bogor, Tangerang, Depok and Jakarta. The sample criteria used were fish and shrimp showing normal and clinical symptoms of being attacked by a disease which were collected by purposive sampling. The collected samples were examined in two stages, namely physical examination of clinical symptoms and molecular examination using PCR. The results showed that of the 24 species samples collected, 17 species showed normal clinical symptoms and seven species showed clinical symptoms. Of the 24 fish and shrimp samples, four samples were infected (2 samples from normal species and 2 samples with clinical symptoms). The infected normal samples were Osphronemus goramy and Litopenaeus vannamei. The infected samples with clinical symptoms were Carassius auratus by Aeromonas salmonicida and red zebra cichlid (Metriaclima estherae) by Red Sea Bream Iridoviral Disease (RSBIV). Physical testing supported by molecular detection of aquatic animals can be an effort to manage aquaculture systems in Indonesia. \u0000 \u0000Keyword: aquaculture, emerging disease, fisheries products, molecular assay. \u0000 \u0000ABSTRAK \u0000 \u0000Penyakit infeksi adalah tantangan utama pada sistem budidaya ikan dan udang yang disebabkan oleh mikroorganisme termasuk bakteri, virus, dan parasit. Deteksi secara fisik memiliki keterbatasan dalam mengetahui lebih dini penyakit pada hewan budidaya, karena gejala klinis yang bervariasi. Tujuan dari penelitian ini adalah untuk melakukan identifikasi fisik dan molekuler keberadaan patogen infeksi pada ikan dan udang yang dibudidayakan di beberapa pertambakan di Jawa Barat, Banten, dan Jakarta. Pemantauan dilakukan dalam dua tahap yaitu periode Maret – April 2021 dan September 2021 dengan mengambil sampel dari beberapa pertambakan di Bogor, Tangerang, Depok, dan Jakarta. Kriteria sampel yang digunakan adalah ikan dan udang yang menunjukkan gejala klinis terserang oleh penyakit yang dikumpulkan secara purposive sampling. Sampel yang terkumpul diperiksa dalam dua tahap yaitu pemeriksaan fisik gejala klinis dan pemeriksaan molekuler menggunakan PCR. Hasil menunjukkan dari 24 sampel spesies yang terkumpul, sebanyak 17 spesies menunjukkan gejala klinis normal dan tujuh spesies menunjukkan gejala klinis. Dari 24 sampel ikan dan udang, terdapat empat sampel yang terinfeksi (2 sampel dari spesies normal dan 2 sampel dengan gejala klinis). Sampel normal yang terinfeksi adalah Osphronemus goramy dan Lito","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"57 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141653378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-10DOI: 10.19027/jai.23.2.122-133
Bagus Ansani Takwin, D. Wahjuningrum, W. Widanarni, Hasan Nasrullah
budidaya udang vaname saat ini karena dapat menyebabkan kematian massal udang. Penggunaan bakteriofage (fage) dapat menjadi alternatif solusi untuk menggantikan antibiotik, karena fage bersifat spesifik pada bakteri tertentu. Penelitian ini meliputi beberapa tahapan, yaitu: isolasi fage dari beberapa sumber air tambak; karakterisasi fage meliputi morfologi plak fage; spot test; uji densitas fage dan uji kisaran inang; serta uji in vitro penghambatan V. parahaemolyticus oleh fage. Uji in vitro yang dilakukan dengan 6 perlakuan dan masing masing 3 ulangan yaitu kontrol negatif (K-), kontrol positif (K+), kontrol antibiotik, kepadatan fage 107 (FB107), kepadatan fage 108 (FB108), dan kepadatan fage FB109 (FB 109). Penelitian ini berhasil mengisolasi fage yang bersumber dari air tambak udang di Banyuwangi ditandai dengan terbentuknya plak pada media double layer agar, dengan dengan ciri morfologisnya berbentuk titik-titik dan bulat besar (diameter 0,008-0,5 cm), dengan tepian rata dan bergelombang serta memiliki warna plak yang bening. Plak fage dapat diperbanyak dengan densitas fage rata-rata 3,5 x 109 PFU mL-1. Untuk uji kisaran inang menggunakan 6 jenis bakteri dengan sumber dan strain yang berbeda, menunjukkan bahwa hanya bakteri V. parahaemolyticus Situbondo yang dapat dilisis oleh fage hasil isolasi, sedangkan 5 jenis bakteri lainnya tidak. Pada uji hambat bakteri, perlakuan dengan penambahan pada setiap densitas fage berbeda nyata dibandingkan dengan kontrol negatif, kontrol media dan kontrol antibiotik, walaupun kontrol antibiotik lebih baik dari perlakuan densitas fage. Semua perlakuan kepadatan fage mampu mengurangi kepadatan V. parahaemolyticus dibandingkan dengan perlakuan kontrol negatif. Hasil terbaik terdapat pada perlakuan fage Banyuwangi 109 PFU mL-1.
{"title":"The potential of bacteriophage for controlling Vibrio parahaemolyticus as in-vitro","authors":"Bagus Ansani Takwin, D. Wahjuningrum, W. Widanarni, Hasan Nasrullah","doi":"10.19027/jai.23.2.122-133","DOIUrl":"https://doi.org/10.19027/jai.23.2.122-133","url":null,"abstract":"budidaya udang vaname saat ini karena dapat menyebabkan kematian massal udang. Penggunaan bakteriofage (fage) dapat menjadi alternatif solusi untuk menggantikan antibiotik, karena fage bersifat spesifik pada bakteri tertentu. Penelitian ini meliputi beberapa tahapan, yaitu: isolasi fage dari beberapa sumber air tambak; karakterisasi fage meliputi morfologi plak fage; spot test; uji densitas fage dan uji kisaran inang; serta uji in vitro penghambatan V. parahaemolyticus oleh fage. Uji in vitro yang dilakukan dengan 6 perlakuan dan masing masing 3 ulangan yaitu kontrol negatif (K-), kontrol positif (K+), kontrol antibiotik, kepadatan fage 107 (FB107), kepadatan fage 108 (FB108), dan kepadatan fage FB109 (FB 109). Penelitian ini berhasil mengisolasi fage yang bersumber dari air tambak udang di Banyuwangi ditandai dengan terbentuknya plak pada media double layer agar, dengan dengan ciri morfologisnya berbentuk titik-titik dan bulat besar (diameter 0,008-0,5 cm), dengan tepian rata dan bergelombang serta memiliki warna plak yang bening. Plak fage dapat diperbanyak dengan densitas fage rata-rata 3,5 x 109 PFU mL-1. Untuk uji kisaran inang menggunakan 6 jenis bakteri dengan sumber dan strain yang berbeda, menunjukkan bahwa hanya bakteri V. parahaemolyticus Situbondo yang dapat dilisis oleh fage hasil isolasi, sedangkan 5 jenis bakteri lainnya tidak. Pada uji hambat bakteri, perlakuan dengan penambahan pada setiap densitas fage berbeda nyata dibandingkan dengan kontrol negatif, kontrol media dan kontrol antibiotik, walaupun kontrol antibiotik lebih baik dari perlakuan densitas fage. Semua perlakuan kepadatan fage mampu mengurangi kepadatan V. parahaemolyticus dibandingkan dengan perlakuan kontrol negatif. Hasil terbaik terdapat pada perlakuan fage Banyuwangi 109 PFU mL-1.","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141660779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-10DOI: 10.19027/jai.23.2.134-146
Nurul Faoziyatunnisa, M. Yuhana, D. Wahjuningrum, Sri Nuryati, Usamah Afiff
This study aims to analyze the administration of microencapsulated probiotic Bacillus cereus BR2 through feed with different doses on the survival, immune response, and digestive enzyme activity of catfish Clarias sp. infected with Aeromonas hydrophila NFC1. The research design used consisted of five treatments and three replications, namely (K-) feeding without the supplementation of probiotics; fish injected with Phosphate-buffered saline (PBS) solution; (K+) feeding without the supplementation of probiotics, fish injected with A. hydrophila NFC1 cell suspension (106 CFU mL-1); (P1) feeding with supplementation of B. cereus BR2 microcapsules at a dose of 0.5% (w/w); (P2) feeding with the supplementation of B. cereus BR2 microcapsules at a dose of 1% (w/w; (P3) feeding with the supplementation of B. cereus BR2 microcapsules at a dose of 2% (w/w). all treatments of P1, P2, and P3 were intramuscularly injected with A. hydrophila NFC1 cell suspension. Catfish fingerlings in average body weight approximately of 3.73 ± 0.22 g were reared in tanks filled with 30 liters of water, and at a density of 15 fish in each container. A feed supplementation trial was performed for 40 days with feeding times three times a day; based on fish biomass feeding rate. Fish rearing was continued after the challenge test with A. hydrophila NFC1 for 10 days. The results showed that the application of supplemented feed containing B. cereus BR2 probiotic microcapsules increased the survival rate, immune response, and digestive enzyme activity of catfish Clarias sp. which was infected with A. hydrophila NFC1, with the best results at 2% microcapsule supplementation.
{"title":"Application of probiotic microcapsules Bacillus cereus BR2 with different doses for the prevention of Aeromonas hydrophila NFC1 infection in catfish Clarias sp.","authors":"Nurul Faoziyatunnisa, M. Yuhana, D. Wahjuningrum, Sri Nuryati, Usamah Afiff","doi":"10.19027/jai.23.2.134-146","DOIUrl":"https://doi.org/10.19027/jai.23.2.134-146","url":null,"abstract":"This study aims to analyze the administration of microencapsulated probiotic Bacillus cereus BR2 through feed with different doses on the survival, immune response, and digestive enzyme activity of catfish Clarias sp. infected with Aeromonas hydrophila NFC1. The research design used consisted of five treatments and three replications, namely (K-) feeding without the supplementation of probiotics; fish injected with Phosphate-buffered saline (PBS) solution; (K+) feeding without the supplementation of probiotics, fish injected with A. hydrophila NFC1 cell suspension (106 CFU mL-1); (P1) feeding with supplementation of B. cereus BR2 microcapsules at a dose of 0.5% (w/w); (P2) feeding with the supplementation of B. cereus BR2 microcapsules at a dose of 1% (w/w; (P3) feeding with the supplementation of B. cereus BR2 microcapsules at a dose of 2% (w/w). all treatments of P1, P2, and P3 were intramuscularly injected with A. hydrophila NFC1 cell suspension. Catfish fingerlings in average body weight approximately of 3.73 ± 0.22 g were reared in tanks filled with 30 liters of water, and at a density of 15 fish in each container. A feed supplementation trial was performed for 40 days with feeding times three times a day; based on fish biomass feeding rate. Fish rearing was continued after the challenge test with A. hydrophila NFC1 for 10 days. The results showed that the application of supplemented feed containing B. cereus BR2 probiotic microcapsules increased the survival rate, immune response, and digestive enzyme activity of catfish Clarias sp. which was infected with A. hydrophila NFC1, with the best results at 2% microcapsule supplementation.","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141661261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ex-mining pit is a wide expanse, often becomes a problem, especially for the environment. Lack of information about the use of mining pits for fishing activities, especially for the studies on the quality of mining pits to support the growth of cultivated fish. This research was conducted in the village of Tanjung Riu, Gunung Mas Regency, Central Kalimantan Province, using two ex-mining ponds belonging to the community. The results show that the water quality for both mine pits with water parameters for: dissolved oxygen (DO) on average 6.09 mg/l – 7.45 mg/l, pH 2.94 – 3.54, Biological Oxygen Demand (BOD) 4.78 mg/L, temperature 30 -32 oC, turbidity 51.7-53.5 NTU, value of Total Dissolved Solids (TDS) 0.003 – 0.017 gr/L, mercury (Hg) content of 0.000247 mg/l - 0.000359 mg/l, the depth of the pool is 1.7 m – 2.74 m. The abundance of plankton is between 869 - 1106 cells/L, and abundance of benthos is between 264 - 352 ind/m2. The range of pond water quality based on second-class national water quality standards, which is suitable for fish cultivation with moderate abundance of plankton.
前矿坑面积广阔，往往成为一个问题，特别是对环境而言。缺乏有关利用矿坑开展渔业活动的信息，特别是有关矿坑质量以支持养殖鱼类生长的研究。本研究在中加里曼丹省 Gunung Mas 县的 Tanjung Riu 村进行，使用了属于该社区的两个前矿坑。结果显示，两个矿坑的水质参数为：溶解氧 (DO) 平均 6.09 mg/l - 7.45 mg/l、pH 值 2.94 - 3.54、生物需氧量 (BOD) 4.78 mg/L、温度 30 - 32 oC、浑浊度 51.7 - 53.浮游生物丰度在 869 - 1106 cells/L 之间，底栖生物丰度在 264 - 352 ind/m2 之间。池塘水质范围基于国家二类水质标准，适合鱼类养殖，浮游生物丰度适中。
{"title":"Study of water quality in ex-mining pits for fish cultivation in Gunung Mas regency, Central Kalimantan","authors":"Lukas Inel, Infa Minggawati, Tania Serezova Augusta, Frid Agustinus, Fenky Wirada","doi":"10.19027/jai.23.1.113-121","DOIUrl":"https://doi.org/10.19027/jai.23.1.113-121","url":null,"abstract":"Ex-mining pit is a wide expanse, often becomes a problem, especially for the environment. Lack of information about the use of mining pits for fishing activities, especially for the studies on the quality of mining pits to support the growth of cultivated fish. This research was conducted in the village of Tanjung Riu, Gunung Mas Regency, Central Kalimantan Province, using two ex-mining ponds belonging to the community. The results show that the water quality for both mine pits with water parameters for: dissolved oxygen (DO) on average 6.09 mg/l – 7.45 mg/l, pH 2.94 – 3.54, Biological Oxygen Demand (BOD) 4.78 mg/L, temperature 30 -32 oC, turbidity 51.7-53.5 NTU, value of Total Dissolved Solids (TDS) 0.003 – 0.017 gr/L, mercury (Hg) content of 0.000247 mg/l - 0.000359 mg/l, the depth of the pool is 1.7 m – 2.74 m. The abundance of plankton is between 869 - 1106 cells/L, and abundance of benthos is between 264 - 352 ind/m2. The range of pond water quality based on second-class national water quality standards, which is suitable for fish cultivation with moderate abundance of plankton.","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"100 48","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140377606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-22DOI: 10.19027/jai.23.1.101-112
Aldi Baihaki, Nur Asiah, Nuraini Nuraini
Cultivation of Pangasianodon hypophthalmus is growing rapidly, but there was a problem with the egg’s adhesiveness. Overcome this problem given a tea solution that serves as a remove of Pangasianodon hypophthalmus egg adhesion. This study conducted on June 2022 at Fish Hatchery and Breeding Laboratory, Faculty of Fisheries and Marine, University of Riau. The purpose of this study was determining the effect of immersion eggs with different doses and immersion duration of the black tea (Camellia sinensis) solution on adhesion of Pangasianodon hypophthalmus eggs. This study used a Completely Randomized Design Factorial with 2 factors. The first factor was the dose (g/L) of the black tea with 4 levels; 0 (D0), 8 (D8), 10 (D10), and 12 (D12). The second factor was the immersion duration (minutes) with three levels of 3 (W3), 4 (W4), and 5 (W5). Pangasianodon hypophthalmus larvae reared for 7 days. Parameters measured were egg adhesion, fertilization rate, hatching rate, survival rate, and water quality. The result showed that the doses of C. sinensis solution had a significant effect (P<0.05) on the degree of fertilization and hatching of Pangasianodon hypophthalmus eggs. The optimum treatment with a dose of 10 g/L and 4 minutes (D10W4) give increased eggs adhesion 5.99%, fertilization rate 94.13%, hatching rate 92.88%, and survival rate 96.99%. The water quality during the study were temperature 26.7-27.9OC, pH of larvae maintenance water 6.5-7.1 and DO 4.0-5.2 mg/L. There is an interaction between dose and immersion duration of C. sinensis solution to the egg adhesion and hatching eggs of Pangasianodon hypophthalmus.
{"title":"The Effect Dose and Soaking Time of Camellia sinensis Solution to the Egg Adhesion and Hatching of Pangasionodon hypophthalmus","authors":"Aldi Baihaki, Nur Asiah, Nuraini Nuraini","doi":"10.19027/jai.23.1.101-112","DOIUrl":"https://doi.org/10.19027/jai.23.1.101-112","url":null,"abstract":"Cultivation of Pangasianodon hypophthalmus is growing rapidly, but there was a problem with the egg’s adhesiveness. Overcome this problem given a tea solution that serves as a remove of Pangasianodon hypophthalmus egg adhesion. This study conducted on June 2022 at Fish Hatchery and Breeding Laboratory, Faculty of Fisheries and Marine, University of Riau. The purpose of this study was determining the effect of immersion eggs with different doses and immersion duration of the black tea (Camellia sinensis) solution on adhesion of Pangasianodon hypophthalmus eggs. This study used a Completely Randomized Design Factorial with 2 factors. The first factor was the dose (g/L) of the black tea with 4 levels; 0 (D0), 8 (D8), 10 (D10), and 12 (D12). The second factor was the immersion duration (minutes) with three levels of 3 (W3), 4 (W4), and 5 (W5). Pangasianodon hypophthalmus larvae reared for 7 days. Parameters measured were egg adhesion, fertilization rate, hatching rate, survival rate, and water quality. The result showed that the doses of C. sinensis solution had a significant effect (P<0.05) on the degree of fertilization and hatching of Pangasianodon hypophthalmus eggs. The optimum treatment with a dose of 10 g/L and 4 minutes (D10W4) give increased eggs adhesion 5.99%, fertilization rate 94.13%, hatching rate 92.88%, and survival rate 96.99%. The water quality during the study were temperature 26.7-27.9OC, pH of larvae maintenance water 6.5-7.1 and DO 4.0-5.2 mg/L. There is an interaction between dose and immersion duration of C. sinensis solution to the egg adhesion and hatching eggs of Pangasianodon hypophthalmus.","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":" 31","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140218915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-01DOI: 10.19027/jai.23.1.92-100
Muhammad Faris Allam, J. Ekasari, I. Fauzi, W. Wiyoto
The different carbohydrate complexities are thought to affect the bioavailability of carbon sources for heterotrophic bacteria in the biofloc formation process and produce different biofloc characteristics. Based on these, the purpose of this study was to evaluate the effect of using carbon sources with different levels of complexity on the characteristics and quality of biofloc in pacific white shrimp (Penaeus vannamei) rearing. The carbon sources used in this study were dextrose (DX), potato starch (ST), and α-cellulose (CL). The experimental tests were the TAN (total ammonia nitrogen) reduction test which was carried out for 16 hours and the shrimp rearing test which was carried out for 30 days. Parameters measured to determine the characteristics of biofloc in shrimp rearing test were bifloc volume, biofloc particle size, biofloc nutrient composition, and water quality. The DX treatment produced the lowest TAN in the TAN reduction test. The shrimp rearing test showed the biofloc had different nutrient quality between the treatments. Meanwhile, the water quality between the treatments had relatively close values, but the CL treatment was able to suppress TAN and NO2 lower than the DX and ST treatments during the rearing period. In addition, it is feared that the biofloc volume of the DX treatment which is higher than the ST and CL treatments is feared to be the main cause of stress in shrimp. The conclusion of this study is that complex carbohydrates, especially cellulose, have more potential in improving the performance of the biofloc system. Keywords: biofloc volume, carbohydrate complexity, nutrient composition, particle size ABSTRAK Kompleksitas karbohidrat diduga dapat memengaruhi bioavaibilitas sumber karbon bagi bakteri heterotrof dalam proses pembentukan bioflok, sehingga kompleksitas karbohidrat yang berbeda diduga dapat menghasilkan karakteristik bioflok yang berbeda. Berdasarkan hal tersebut maka tujuan dari penelitian ini adalah untuk mengevaluasi pengaruh penggunaan sumber karbon dengan tingkat kompleksitas yang berbeda terhadap karakteristik dan kualitas bioflok pada pemeliharaan udang vaname (Penaeus vannamei). Sumber karbon yang digunakan dalam penelitian ini adalah dekstrosa (DX), tepung kentang (ST), dan α-selulosa (CL) dan rancangan percobaan yang digunakan adalah RAL (rancangan acak lengkap). Penelitian ini memiliki dua jenis pengujian yang dilakukan secara paralel, yaitu uji penurunan TAN (total ammonia nitrogen) yang dilakukan selama 16 jam dan uji pemeliharaan udang yang dilakukan selama 30 hari. Parameter yang diukur untuk mengetahui karakteristik bioflok pada pemeliharaan udang adalah volume biflok, ukuran partikel bioflok, komposisi nutrien bioflok, dan kualitas air pemeliharaan. Perlakuan DX menghasilkan TAN paling rendah pada uji penurunan TAN. Bioflok yang dihasilkan dari uji pemeliharaan udang menunjukkan kualitas nutrien bioflok yang berbeda-beda antar perlakuanya. Sementara itu kualitas air antar per
不同的碳水化合物复杂程度被认为会影响生物絮团形成过程中异养细菌对碳源的生物利用率，并产生不同的生物絮团特性。因此，本研究的目的是评估在太平洋南美白对虾（Penaeus vannamei）饲养过程中使用不同复杂程度的碳源对生物絮团特性和质量的影响。本研究使用的碳源为葡萄糖（DX）、马铃薯淀粉（ST）和α-纤维素（CL）。实验测试包括 16 小时的 TAN（总氨氮）还原测试和 30 天的对虾饲养测试。在对虾饲养试验中，为确定生物絮团的特性而测量的参数包括双胞体积、生物絮团粒径、生物絮团营养成分和水质。在 TAN 降低试验中，DX 处理产生的 TAN 最低。对虾饲养试验表明，不同处理的生物絮营养质量不同。同时，各处理之间的水质值比较接近，但在饲养期间，CL 处理对 TAN 和 NO2 的抑制作用低于 DX 和 ST 处理。此外，DX 处理的生物絮团体积高于 ST 和 CL 处理，这恐怕是造成对虾应激的主要原因。本研究的结论是，复合碳水化合物，尤其是纤维素，在改善生物絮凝物系统的性能方面具有更大的潜力。 关键词：生物絮团体积、碳水化合物复杂性、营养成分、粒径 ABSTRACT Carbohydrate complexity is thought to affect the biofloc formation process of carbon sources for heterotrophic bacteria, so different carbohydrate complexities are thought to produce different biofloc characteristics.基于此，本研究的目的是评估在饲养凡纳滨对虾（Penaeus vannamei）的过程中，使用不同复杂程度的碳源对生物絮团的特性和质量的影响。本研究使用的碳源为葡萄糖（DX）、马铃薯淀粉（ST）和α-纤维素（CL），实验设计为完全随机设计（RAL）。本研究同时进行了两类试验，即 TAN（总氨氮）降低试验（进行 16 小时）和虾饲养试验（进行 30 天）。为确定生物絮团在对虾饲养中的特性而测量的参数包括双絮体体积、生物絮团粒径、生物絮团营养成分和饲养水质。在 TAN 降低试验中，DX 处理产生的 TAN 最低。对虾饲养试验产生的生物絮凝物显示出不同处理间生物絮凝物营养质量的差异。虽然各处理之间的水质值比较接近，但在饲养期间，CL 处理对 TAN 和 NO2 的抑制作用低于 DX 和 ST 处理。此外，DX 处理的最终生物絮体体积高于 ST 和 CL 处理，这恐怕是造成对虾应激的主要原因。本研究的结论是，复合碳水化合物，尤其是纤维素，更有潜力改善生物絮凝物系统的性能。 关键词：碳水化合物的复杂性、营养成分、粒度、生物絮团体积
{"title":"Characteristics and quality of biofloc in vannamei shrimp culture with different carbon sources","authors":"Muhammad Faris Allam, J. Ekasari, I. Fauzi, W. Wiyoto","doi":"10.19027/jai.23.1.92-100","DOIUrl":"https://doi.org/10.19027/jai.23.1.92-100","url":null,"abstract":"The different carbohydrate complexities are thought to affect the bioavailability of carbon sources for heterotrophic bacteria in the biofloc formation process and produce different biofloc characteristics. Based on these, the purpose of this study was to evaluate the effect of using carbon sources with different levels of complexity on the characteristics and quality of biofloc in pacific white shrimp (Penaeus vannamei) rearing. The carbon sources used in this study were dextrose (DX), potato starch (ST), and α-cellulose (CL). The experimental tests were the TAN (total ammonia nitrogen) reduction test which was carried out for 16 hours and the shrimp rearing test which was carried out for 30 days. Parameters measured to determine the characteristics of biofloc in shrimp rearing test were bifloc volume, biofloc particle size, biofloc nutrient composition, and water quality. The DX treatment produced the lowest TAN in the TAN reduction test. The shrimp rearing test showed the biofloc had different nutrient quality between the treatments. Meanwhile, the water quality between the treatments had relatively close values, but the CL treatment was able to suppress TAN and NO2 lower than the DX and ST treatments during the rearing period. In addition, it is feared that the biofloc volume of the DX treatment which is higher than the ST and CL treatments is feared to be the main cause of stress in shrimp. The conclusion of this study is that complex carbohydrates, especially cellulose, have more potential in improving the performance of the biofloc system. \u0000 \u0000Keywords: biofloc volume, carbohydrate complexity, nutrient composition, particle size \u0000 \u0000ABSTRAK \u0000 \u0000Kompleksitas karbohidrat diduga dapat memengaruhi bioavaibilitas sumber karbon bagi bakteri heterotrof dalam proses pembentukan bioflok, sehingga kompleksitas karbohidrat yang berbeda diduga dapat menghasilkan karakteristik bioflok yang berbeda. Berdasarkan hal tersebut maka tujuan dari penelitian ini adalah untuk mengevaluasi pengaruh penggunaan sumber karbon dengan tingkat kompleksitas yang berbeda terhadap karakteristik dan kualitas bioflok pada pemeliharaan udang vaname (Penaeus vannamei). Sumber karbon yang digunakan dalam penelitian ini adalah dekstrosa (DX), tepung kentang (ST), dan α-selulosa (CL) dan rancangan percobaan yang digunakan adalah RAL (rancangan acak lengkap). Penelitian ini memiliki dua jenis pengujian yang dilakukan secara paralel, yaitu uji penurunan TAN (total ammonia nitrogen) yang dilakukan selama 16 jam dan uji pemeliharaan udang yang dilakukan selama 30 hari. Parameter yang diukur untuk mengetahui karakteristik bioflok pada pemeliharaan udang adalah volume biflok, ukuran partikel bioflok, komposisi nutrien bioflok, dan kualitas air pemeliharaan. Perlakuan DX menghasilkan TAN paling rendah pada uji penurunan TAN. Bioflok yang dihasilkan dari uji pemeliharaan udang menunjukkan kualitas nutrien bioflok yang berbeda-beda antar perlakuanya. Sementara itu kualitas air antar per","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":" 540","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140092543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Muhammad Rasnijal, Ardana Kurniaji, Anton Anton, Budiyati Budiyati, Diana Putri Renitasari, Achmad Suhermanto, Mugi Mulyono, I. S. Djunaidah, Sinung Rahardjo, Sinar Pagi Sektiana, Ridwan Ridwan
Seaweed (Kappaphycus alvarezii) could be a source of carrageenan needed for industrial sector. The purpose of this study to analyze the characteristics of carrageenan seaweed cultivated with bag and non-bag cultivation systems and different seed weights. The research was conducted in the waters of Bone Bay and the Laboratory of the Indonesian Center for Brackish Water Cultivation and Fisheries Extension in Maros. This study used a factorial completely randomized design (CRD) consisting of factor A (using bags and non-bags), and factor B (seed weight 15, 50, 75, 100, and 125 g). The results showed that the cultivation method factor, seed weight factor, and their interaction had an effect on carrageenan yield, gel strength, ash content and water content (P <0.05). Different cultivation systems with different initial seed weight combinations showed different best results for each parameter. The highest value of yield of carrageenan was observed in the bag culture system with a seed weight of 100 g, namely 29.18 ± 1.10%. The highest value of gel strength was observed in the non-bagged cultivation system with a seed weight of 75 g, namely 1344.69 ± 18.43 g/cm2. The highest value of ash content was found in the non-bagged cultivation system with 125 g of seed weight, namely 30.02 ± 0.13%. The highest value of water content was found in the bag culture system at a seed weight of 15 g, namely 38.63 ± 0.26%. Different cultivation methods and seed weight resulted in other carrageenan characteristics of seaweed for each parameter. Keywords: gel strength, ash content, moisture content, yield ABSTRAK Rumput laut (Kappaphycus alvarezii) memiliki potensi sebagai sumber karaginan yang banyak dibutuhkan untuk bidang industri. Penelitian ini bertujuan untuk menganalisis karakteristik karaginan rumput laut yang dibudidayakan dengan sistem budidaya kantong dan non kantong serta berat bibit yang berbeda. Penelitian dilaksanakan di perairan Teluk Bone dan Laboratorium Balai Riset Budidaya Air Payau dan Penyuluhan Peikanan Maros. Percobaan penelitian dilakukan melalui rancangan acak lengkap (RAL) faktorial yang terdiri faktor A (memakai kantong dan non kantong), faktor B (berat bibit 15, 50, 75, 100, dan 125 g). Hasil penelitian menunjukkan bahwa faktor metode budidaya, berat bibit dan interaksi keduanya berpengaruh terhadap rendeman karaginan, kekuatan gel, kadar air, kadar abu (P<0,05). Penggunaan sistem budidaya yang berbeda dengan kombinasi berat awal bibit berbeda menunjukkan hasil terbaik yang tidak sama pada tiap parameter. Nilai tertinggi rendeman keraginan teramati pada sistem budidaya kantong dengan berat bibit 100 g yakni 29,18 ± 1,10 %. Nilai tertinggi kekuatan gel teramati pada sistem budidaya non kantong dengan berat bibit 50 g yaitu 1344,69 ± 18,43 g/cm2. Nilai kadar abu tertinggi pada sistem budidaya non kantong dengan berat bibit 125 g yaitu 30,02 ± 0,13%. Nilai kadar air tertinggi pada sistem budidaya kantong dengan berat bibit 15 g yaitu
海藻（Kappaphycus alvarezii）可作为工业部门所需的卡拉胶来源。本研究的目的是分析用袋式和非袋式栽培系统以及不同种子重量栽培的卡拉胶海藻的特性。研究在马罗斯的骨湾水域和印度尼西亚咸水养殖和渔业推广中心实验室进行。该研究采用了因子完全随机设计（CRD），包括因子 A（使用袋和不使用袋）和因子 B（种子重量 15、50、75、100 和 125 克）。结果表明，栽培方法因子、种子重量因子及其交互作用对卡拉胶产量、凝胶强度、灰分含量和含水量均有影响（P <0.05）。不同的栽培系统和不同的初始种子重量组合对各参数都显示出不同的最佳结果。在种子重量为 100 克的袋栽培系统中，卡拉胶产量最高，为 29.18 ± 1.10%。种子重量为 75 克的非袋装栽培系统的凝胶强度值最高，为 1344.69 ± 18.43 克/平方厘米。种子重量为 125 克的非袋装栽培系统的灰分含量最高，为 30.02 ± 0.13%。在种子重量为 15 克的袋栽培系统中，水分含量最高，为 38.63 ± 0.26%。不同的培养方法和种子重量导致海藻的卡拉胶特性各不相同。 关键词：凝胶强度、灰分含量、水分含量、产量 ABSTRAK Rumput laut (Kappaphycus alvarezii) memiliki potensi sebagai sumber karaginan yang banyak dibutuhkan untuk bidang industri.它的作用是管理从传统和非传统的植物系统中分离出来的茎叶植物，以及植物的茎叶。在特卢克骨和巴尤岛空气污染与马罗斯山脉空气污染实验室进行了检测。通过 A 项（记住重量和不记住重量）、B 项（重量分别为 15、50、75、100 和 125 克）的 RAL（Rancangan acak lengkap）实验来确定检测项目。实验结果表明，芽苞模式、茎秆重量和茎秆间距对茎秆重量、凝胶重量、空气重量和茎秆重量的影响均小于 0.05（P<0.05）。与其他参数相比，该系统具有更高的可靠性。100 克体重的芽孔系统的平均畸变率为 29.18 ± 1.10 %。在 50 克重的非肛门系统中，凝胶的凝结率为 1344.69 ± 18.43 克/平方厘米。在 125 克重的非悬挂式喷射系统中，空气中的凝胶含量为 30.02 ± 0.13%。在 15 克重的芽苞生长系统中，空气的比例为 38.63 ± 0.26%。新陈代谢法和掺入量的计算显示，在不同的参数下，新陈代谢法和掺入量的计算显示了不同的结果。 关键字：凝胶、空气、渲染
{"title":"Characteristics of seaweed caraginan Kappaphycus alvarezii on cultivation system with different seed weight","authors":"Muhammad Rasnijal, Ardana Kurniaji, Anton Anton, Budiyati Budiyati, Diana Putri Renitasari, Achmad Suhermanto, Mugi Mulyono, I. S. Djunaidah, Sinung Rahardjo, Sinar Pagi Sektiana, Ridwan Ridwan","doi":"10.19027/jai.23.1.71-78","DOIUrl":"https://doi.org/10.19027/jai.23.1.71-78","url":null,"abstract":"Seaweed (Kappaphycus alvarezii) could be a source of carrageenan needed for industrial sector. The purpose of this study to analyze the characteristics of carrageenan seaweed cultivated with bag and non-bag cultivation systems and different seed weights. The research was conducted in the waters of Bone Bay and the Laboratory of the Indonesian Center for Brackish Water Cultivation and Fisheries Extension in Maros. This study used a factorial completely randomized design (CRD) consisting of factor A (using bags and non-bags), and factor B (seed weight 15, 50, 75, 100, and 125 g). The results showed that the cultivation method factor, seed weight factor, and their interaction had an effect on carrageenan yield, gel strength, ash content and water content (P <0.05). Different cultivation systems with different initial seed weight combinations showed different best results for each parameter. The highest value of yield of carrageenan was observed in the bag culture system with a seed weight of 100 g, namely 29.18 ± 1.10%. The highest value of gel strength was observed in the non-bagged cultivation system with a seed weight of 75 g, namely 1344.69 ± 18.43 g/cm2. The highest value of ash content was found in the non-bagged cultivation system with 125 g of seed weight, namely 30.02 ± 0.13%. The highest value of water content was found in the bag culture system at a seed weight of 15 g, namely 38.63 ± 0.26%. Different cultivation methods and seed weight resulted in other carrageenan characteristics of seaweed for each parameter. \u0000 \u0000Keywords: gel strength, ash content, moisture content, yield \u0000 \u0000ABSTRAK \u0000 \u0000Rumput laut (Kappaphycus alvarezii) memiliki potensi sebagai sumber karaginan yang banyak dibutuhkan untuk bidang industri. Penelitian ini bertujuan untuk menganalisis karakteristik karaginan rumput laut yang dibudidayakan dengan sistem budidaya kantong dan non kantong serta berat bibit yang berbeda. Penelitian dilaksanakan di perairan Teluk Bone dan Laboratorium Balai Riset Budidaya Air Payau dan Penyuluhan Peikanan Maros. Percobaan penelitian dilakukan melalui rancangan acak lengkap (RAL) faktorial yang terdiri faktor A (memakai kantong dan non kantong), faktor B (berat bibit 15, 50, 75, 100, dan 125 g). Hasil penelitian menunjukkan bahwa faktor metode budidaya, berat bibit dan interaksi keduanya berpengaruh terhadap rendeman karaginan, kekuatan gel, kadar air, kadar abu (P<0,05). Penggunaan sistem budidaya yang berbeda dengan kombinasi berat awal bibit berbeda menunjukkan hasil terbaik yang tidak sama pada tiap parameter. Nilai tertinggi rendeman keraginan teramati pada sistem budidaya kantong dengan berat bibit 100 g yakni 29,18 ± 1,10 %. Nilai tertinggi kekuatan gel teramati pada sistem budidaya non kantong dengan berat bibit 50 g yaitu 1344,69 ± 18,43 g/cm2. Nilai kadar abu tertinggi pada sistem budidaya non kantong dengan berat bibit 125 g yaitu 30,02 ± 0,13%. Nilai kadar air tertinggi pada sistem budidaya kantong dengan berat bibit 15 g yaitu ","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"35 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139781720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：