DEVELOPMENT AND VALIDATION OF STABILITY INDICATING HPTLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF METFORMIN HYDROCHLORIDE AND PIOGLITAZONE IN PHARMACEUTICAL DOSAGE FORM

Abstract

A simple, specific, accurate and precise High Performance Thin Layer Chromatography (HPTLC) method was developed which can separate and quantitatively estimate formulation of Metformin Hydrochloride and Pioglitazone The chromatographic separation for Metformin Hydrochloride and Pioglitazone in HPTLC method separation was achieved with the mobile phase consisting of Toluene: Methanol: Triethylamine (6: 4: 0.1 % v/v/v/v). The detection of spot was carried out at 230 nm and the Rf values Metformin Hydrochloride and Pioglitazone were found to be 0.25±0.03 and 0.47±0.04.. Ths method was validated accordance with ICH guidelines. The recovery these combination were above 96%. The developed method are free from interference due to excipients present in tablets. These method are rapid and simple, do not require any sample preparation and is suitable for routine quality control analysis Keywords: HPTLC ,Method validation, Quantitative analysis