Anti-cancer efficacy of ethanolic extracts from various parts of Annona Squamosa on MCF-7 cell line

Journal of Pharmacognosy and Phytotherapy Pub Date : 2016-07-31 DOI:10.5897/JPP2016.0398

S. Veerakumar, Safreen Shaikh Dawood Amanulla, K. Ramanathan

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引用次数: 7

Abstract

Medicinal plant extracts are known to possess breast cancer antidote. The present investigation is focused on anticancer efficacy of various parts of Annona squamosa. The organic (ethanol) extracts from various parts of Annona squamosa were prepared using soxhlet apparatus and tested for in vitro anticancer efficacy on Breast cancer cell line MCF-7 by MTT (3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The results obtained from MTT assay showed that the inhibitory concentration values of bark, peel and seed were found to be approximately 20, 30 and 10 μg/ml, respectively The ethanolic seed extract had high anticancer activity with IC50 value of 10 ug/ml, reveals that A. squamosa inhibits the proliferation of MCF-7 by inducing apoptosis. The plant investigated has anti-cancer activity; hence further studies should be carried out for the isolation of the lead molecules from the parts of the plant to treat the breast cancer. Key words: Annona squamosa, MCF-7 cell line, 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, anti-cancer activity.

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番荔枝不同部位乙醇提取物对MCF-7细胞的抗癌作用

已知药用植物提取物具有乳腺癌解药。本文对番荔枝各部位的抗癌作用进行了研究。采用索氏装置制备了番槐各部位的有机(乙醇)提取物，并采用MTT(3-(4,5 -二甲基噻唑-2-基)-2,5-二苯基溴化四唑)法检测了其对乳腺癌细胞株MCF-7的体外抗癌作用。MTT法检测结果显示，鳞片树皮、果皮和种子的抑制浓度分别约为20、30和10 μg/ml，籽粒提取物的IC50值为10 μg/ml，具有较高的抗癌活性，表明鳞片提取物通过诱导细胞凋亡抑制MCF-7的增殖。所研究的植物具有抗癌活性;因此，从植物部分分离铅分子以治疗乳腺癌的研究有待进一步开展。关键词:番荔枝，MCF-7细胞系，3-(4,5 -二甲基噻唑-2-基)-2,5-二苯基溴化四唑(MTT)测定，抗癌活性

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Journal of Pharmacognosy and Phytotherapy

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