Leguminous Flour from the Native Argentinian Forest, Their Contribution to Antioxidant Defense

G. S, C. K., Rosas D, Lescano N, M. S.
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Abstract

The oxygen-reactive species may generate oxidative stress, which could result in degenerative diseases. Antioxidant mechanisms usually act in a coordinated way, and they are grouped in two defense systems: enzymatic and nonenzymatic system. The metallo-dependent enzymes of the organism and the natural substances present in legumes have the capacity to delay, decrease or inhibit the oxidative processes. This work aimed to evaluate the bioaccessibility of zinc and bioactive compounds of flour obtained from Argentinian native forest fruits corresponding to the family of leguminous plants: white carob (Prosopis alba) and Chañar (Geoffroea decorticans), in order to use them in human food. The study was done on carob flour (CF) and chañar flour (CHF). The minerals were quantified by atomic absorption spectrometry. Bioaccessibility (D%) was estimated by dialysate percentage after in vitro digestion. The potential contribution (PC) was calculated. Their phenolic concentrations were obtained using the Folin Ciocalteu method and their antioxidant activity was evaluated in vitro using the radical DPPH (1,1-diphenil-2-pichrihydracil) and expressed as the percentage of the trapping capacity against DPPH. CF and CHF presented contents of Zn of 1.20 and 1.48 mg / 100g; D% 24 and 28; PC 0.29 and 0.41 respectively. CHF contains 112 ± 18.3mg EAG/100g of total phenols and AF 156 ± 16.9 mg EAG/100g. The results showed that the antioxidant potential was higher in CHF 39.78% than CF: 16.4%, in preparations with 100 mg/l. The values for the discoloring percentage of DPPH radical were corrected considering the standard quercetine with a value of 100% antioxidant capacity. The samples under study are good sources of Zn, cofactor of the enzyme superoxide dismutase and of antioxidants, mainly of phenolic compounds. Therefore, these flours could be suitable for functional foods formulation. ISSN 2641-4295 Research Article Citation: Generoso S, Costa K, Rosas D, et al. Leguminous Flour from the Native Argentinian Forest, Their Contribution to Antioxidant Defense. Food Sci Nutr Res. 2019; 2(1): 1-5.
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来自阿根廷原生森林的豆科面粉及其对抗氧化防御的贡献
氧活性物质可能产生氧化应激,从而导致退行性疾病。抗氧化机制通常以协调的方式起作用,它们分为两个防御系统:酶和非酶系统。生物体的金属依赖酶和豆类中存在的天然物质具有延迟、减少或抑制氧化过程的能力。本研究旨在评价从阿根廷原生森林果实中提取的面粉中锌和生物活性化合物的生物可及性,这些果实对应于豆科植物:白角豆(Prosopis alba)和Chañar (Geoffroea decorticans),以便将它们用于人类食品中。以角豆粉(CF)和chañar粉(CHF)为原料进行了研究。用原子吸收光谱法对矿物进行了定量分析。通过体外消化后的透析液百分比估算生物可及性(D%)。计算了潜在贡献(PC)。用Folin Ciocalteu法测定其酚类浓度,并用自由基DPPH(1,1-二苯-2-pichrihydracil)评价其体外抗氧化活性,并以捕获DPPH能力的百分比表示。CF和CHF的Zn含量分别为1.20和1.48 mg / 100g;D% 24和28;PC分别为0.29和0.41。CHF含总酚112±18.3mg EAG/100g, AF含总酚156±16.9 mg EAG/100g。结果表明,当浓度为100 mg/l时,CHF含量为39.78%,CF含量为16.4%;考虑到标准槲皮素的抗氧化能力为100%,对DPPH自由基脱色率进行了修正。所研究的样品是锌、酶超氧化物歧化酶的辅助因子和抗氧化剂(主要是酚类化合物)的良好来源。因此,这些面粉可用于功能性食品的配方。研究论文来源:Generoso S, Costa K, Rosas D,等。来自阿根廷原生森林的豆科面粉及其对抗氧化防御的贡献。食品科学与营养杂志,2019;2(1): 1 - 5。
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