A preliminary study of the bacteriological evaluation of raw milk from Kogi State University dairy farm

Z. Azeez, E. Yakubu, S. Enemuor, S. Momoh
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Abstract

Milk is a food that inherently favours microbial growth and due to its characteristics, several precautions must be taken to prevent contamination in its production, processing and consumption, which are routinely subject to changes. Raw milk is a complex mixture which is highly nutritious, contain high level of water (85%) and a pH close to neutral which makes it highly perishable and a suitable medium for the growth and multiplication of microorganisms. This study was carried out to evaluate the bacteria associated with raw milk from a dairy farm. A total of seven (7) raw milk samples were -gotten from different lactating cows. The samples were examined for pH, organoleptic property, and turbidity. Isolation of bacteria was done using the pour plate method, gram staining and various biochemical test were conducted to identify the organisms. The pH value of the samples ranged from 6.5 to 6.9. The total bacterial count ranged from 4.3x106 to 9.0x104 CFU/ml, and total coliform count ranges from 5.1x106 to 4.0x104 the bacteria isolated were Bacillus spp Escherichia coli, Staphylococcus aureus, Klebsiella spp and Streptococcus spp. The high bacteria count obtained in this study is an indication of poor sanitary condition and this calls for strict hygienic measure during handling of raw milk and its products.
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科吉州立大学奶牛场原料奶细菌学评价的初步研究
牛奶是一种天生有利于微生物生长的食物,由于其特性,必须采取一些预防措施,以防止在其生产、加工和消费过程中受到污染,这些措施通常会发生变化。原料奶是一种复杂的混合物,营养丰富,含水量高(85%),pH值接近中性,极易腐烂,是微生物生长和繁殖的合适培养基。本研究的目的是评估与某奶牛场原料奶有关的细菌。共从不同的泌乳奶牛中获得7份原料奶样品。检测样品的pH值、感官特性和浊度。采用淋板法分离细菌,革兰氏染色及各种生化试验对细菌进行鉴定。样品的pH值在6.5 ~ 6.9之间。细菌总数在4.3 × 106 ~ 9.0x104 CFU/ml之间,大肠菌群总数在5.1 × 106 ~ 4.0 × 104之间,分离出的细菌为大肠杆菌、金黄色葡萄球菌、克雷伯氏菌和链球菌。本研究中细菌数量较高,说明卫生条件较差,要求在原料奶及其制品的处理过程中采取严格的卫生措施。
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