Genetic polymorphism of soybean genotypes with contrasting levels of phosphatidylcholine, protein, and lipoxygenase-2

Abstract

For the mapping of quantitative trait loci associated with a particular trait or introgression of desirable traits into a variety from donor parent through marker-assisted backcross breeding, parental polymorphism survey is the first prerequisite. In the present study, a total of 695 simple sequence repeats (SSR) markers spanning over 20 linkage groups were surveyed for parental polymorphism in 5 parental combinations comprising 7 soybean genotypes, namely NRC151, PS1476, IC275, IC574373, AVKS215, JS20-34, and JS20-98, with varying levels of phosphatidylcholine (PC), protein, and lipoxygenase-2. Five different parental combinations, namely NRC151 × IC275, PS1476 × IC275, NRC151 × IC574373, JS20-34 × AVKS215, and JS20-98 × AVKS215, exhibited 40.43%, 43.02%, 36.26%, 39.42%, and 41.29% parental polymorphisms, respectively. Polymorphic markers identified in these parental combinations can be utilized for genotyping of second filial generation (F2)/ recombinant inbred lines (RILs) population generated for the identification of genomic regions associated with PC, protein content, and for the recovery of recurrent parent genome content of respective recurrent parent for development of lipoxygenase-2 free high-yielding soybean genotypes.