Carbon nanoparticles (CNPs) are carbon-based nanomaterial with dimensions in the range of 1–100 nm. In the present research, an ecofriendly, simple, and highly reproducible method was used to prepare the CNPs from the soot of clarified butter (carbon dots) and mustard oil (carbon nanospheres) in both pristine and oxidized forms. The obtained CNPs were subjected to various analyses such as UV-visible, Fourier transform infrared (FTIR), dynamic light scattering, high-resolution transmission electron microscopy, energy-dispersive X-ray, and X-ray diffraction (XRD). The analyses demonstrate that the size of butter-originated CNPs was found in the ranges of 10–90 nm (raw) and 5–20 nm (oxidized), whereas, in the case of mustard oil-originated CNPs, the size was observed in the ranges of 100–150 nm (raw) and 50–80 nm (oxidized). As per zeta potential results, the net surface charges on CNPs were observed as −9.05 and −14.6 mV in the case of raw and oxidized CNPs from butter, respectively, and −12.7 and −20.1 mV in the case of raw and oxidized CNPs from mustard oil, respectively. XRD results showed the typical graphitic crystalline nature of both kinds of CNPs irrespective of their initial raw material. FTIR results confirmed hydroxyl, carboxyl, carbonyl, and amide groups on CNPs that help in their capping and stabilization in the solvent media. Five bacterial strains, Staphylococcus aureus , Escherichia coli , Staphylococcus epidermidis , Klebsiella pneumoniae , and Moraxella catarrhalis , were used to assess the bactericidal potential of synthesized CNPs using agar-well and 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2 H - tetrazolium-5-carboxanilide-colorimetric methods. Butter-mediated oxidized CNPs were the most effective bactericidal agent against all the bacterial strains compared to mustard-originated CNPs. Furthermore, CNPs-mediated toxicity towards bacteria was both size and concentration dependent. Staphylococcus aureus and S. epidermidis were the most sensitive [minimum inhibitory concentration (MIC): 800 µg/ml] and resistant (MIC: 2.0 mg/ml) bacteria, respectively, towards CNPs-mediated toxicity. The synthesized CNPs were devoid of any metallic impurities and hence worthy of being used in various applications like imaging, labeling, sensor-technology, and environment monitoring and as an antibacterial agent.
{"title":"Characterization and comparative assessment of bactericidal activity of carbon nanodots (CDs) and nanoparticles (CNPs) prepared from soot's of clarified butter and mustard oil, respectively","authors":"Vikas Pahal, Pankaj Kumar, Rahul Kumar, Parveen Kumar, Vinod Kumar","doi":"10.7324/jabb.2023.115277","DOIUrl":"https://doi.org/10.7324/jabb.2023.115277","url":null,"abstract":"Carbon nanoparticles (CNPs) are carbon-based nanomaterial with dimensions in the range of 1–100 nm. In the present research, an ecofriendly, simple, and highly reproducible method was used to prepare the CNPs from the soot of clarified butter (carbon dots) and mustard oil (carbon nanospheres) in both pristine and oxidized forms. The obtained CNPs were subjected to various analyses such as UV-visible, Fourier transform infrared (FTIR), dynamic light scattering, high-resolution transmission electron microscopy, energy-dispersive X-ray, and X-ray diffraction (XRD). The analyses demonstrate that the size of butter-originated CNPs was found in the ranges of 10–90 nm (raw) and 5–20 nm (oxidized), whereas, in the case of mustard oil-originated CNPs, the size was observed in the ranges of 100–150 nm (raw) and 50–80 nm (oxidized). As per zeta potential results, the net surface charges on CNPs were observed as −9.05 and −14.6 mV in the case of raw and oxidized CNPs from butter, respectively, and −12.7 and −20.1 mV in the case of raw and oxidized CNPs from mustard oil, respectively. XRD results showed the typical graphitic crystalline nature of both kinds of CNPs irrespective of their initial raw material. FTIR results confirmed hydroxyl, carboxyl, carbonyl, and amide groups on CNPs that help in their capping and stabilization in the solvent media. Five bacterial strains, Staphylococcus aureus , Escherichia coli , Staphylococcus epidermidis , Klebsiella pneumoniae , and Moraxella catarrhalis , were used to assess the bactericidal potential of synthesized CNPs using agar-well and 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2 H - tetrazolium-5-carboxanilide-colorimetric methods. Butter-mediated oxidized CNPs were the most effective bactericidal agent against all the bacterial strains compared to mustard-originated CNPs. Furthermore, CNPs-mediated toxicity towards bacteria was both size and concentration dependent. Staphylococcus aureus and S. epidermidis were the most sensitive [minimum inhibitory concentration (MIC): 800 µg/ml] and resistant (MIC: 2.0 mg/ml) bacteria, respectively, towards CNPs-mediated toxicity. The synthesized CNPs were devoid of any metallic impurities and hence worthy of being used in various applications like imaging, labeling, sensor-technology, and environment monitoring and as an antibacterial agent.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":"141 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135101981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microbes are stated to be an excellent source of novel enzymes and secondary metabolites having potential industrial applications. Many attempts have been made around the globe to unravel novel bioactive molecules from microbes inhabiting different unexplored ecological niches. Myxobacteria, a member of δ -proteobacteria belonging to the order Myxococcales, is a gram-negative rod-shaped bacterium well-known for coordinated movement, fruiting body formation during starvation, gliding mobility, and a well-developed communication system. They have emerged as valuable producers of various natural products belonging to diverse chemical classes with wide-spectrum and unique bioactive molecules that have attracted academicians and researchers for their exploration. The bioactive molecules obtained from myxobacteria have been proclaimed to have the ability to break down macromolecules of biological origin by secreting a large number of extra-cellular enzymes. The current study reports the isolation of myxobacteria from the Mirgund Wetland in the North-Western Himalayas through the dung-baiting method. The isolated myxobacteria showed characteristic morphological features of myxobacteria, like the formation of fruiting bodies and swarming patterns on the solid Casitone-enriched media. 16S ribosomal RNA gene sequencing of the strain showed 100% similarity with Myxococcus spp. The isolated strain produced an active cellulase with an optimum activity at 40°C and pH 7.0 using the 3,5-dinitro salicylic acid method when cultured on minimal broth containing carboxymethyl cellulose. This is the first report of a myxobacterial strain and enzyme from the Mirgund Wetland of the Union Territory of Jammu and Kashmir and highlights the immense potential of the hidden wealth of enzymes in the area.
{"title":"Isolation and Characterization of Cellulase-Producing Myxobacterial Strain from the Unique Niche of Mirgund Wetland from the North-Western Himalayas","authors":"Daljeet Singh Dhanjal, Simranjeet Singh, Vijay Kumar, Praveen C. Ramamurthy, Chirag Chopra, Atif Khurshid Wani, Reena Singh, Joginder Singh","doi":"10.7324/jabb.2023.11514-1","DOIUrl":"https://doi.org/10.7324/jabb.2023.11514-1","url":null,"abstract":"Microbes are stated to be an excellent source of novel enzymes and secondary metabolites having potential industrial applications. Many attempts have been made around the globe to unravel novel bioactive molecules from microbes inhabiting different unexplored ecological niches. Myxobacteria, a member of δ -proteobacteria belonging to the order Myxococcales, is a gram-negative rod-shaped bacterium well-known for coordinated movement, fruiting body formation during starvation, gliding mobility, and a well-developed communication system. They have emerged as valuable producers of various natural products belonging to diverse chemical classes with wide-spectrum and unique bioactive molecules that have attracted academicians and researchers for their exploration. The bioactive molecules obtained from myxobacteria have been proclaimed to have the ability to break down macromolecules of biological origin by secreting a large number of extra-cellular enzymes. The current study reports the isolation of myxobacteria from the Mirgund Wetland in the North-Western Himalayas through the dung-baiting method. The isolated myxobacteria showed characteristic morphological features of myxobacteria, like the formation of fruiting bodies and swarming patterns on the solid Casitone-enriched media. 16S ribosomal RNA gene sequencing of the strain showed 100% similarity with Myxococcus spp. The isolated strain produced an active cellulase with an optimum activity at 40°C and pH 7.0 using the 3,5-dinitro salicylic acid method when cultured on minimal broth containing carboxymethyl cellulose. This is the first report of a myxobacterial strain and enzyme from the Mirgund Wetland of the Union Territory of Jammu and Kashmir and highlights the immense potential of the hidden wealth of enzymes in the area.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136028064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.7324/jabb.2023.131653
Kamalendu De, Dipankar Ghosh
A review on fish peptides isolated from fish waste with their potent bioactivitiesAyusman Behera, Rajashree Das, Pranati Patnaik, Jyotirmaya Mohanty, Gargee Mohanty
{"title":"Isolation and biochemical characterization of natural algal isolates from Kangsabati River, West Bengal toward single-cell proteins production","authors":"Kamalendu De, Dipankar Ghosh","doi":"10.7324/jabb.2023.131653","DOIUrl":"https://doi.org/10.7324/jabb.2023.131653","url":null,"abstract":"A review on fish peptides isolated from fish waste with their potent bioactivitiesAyusman Behera, Rajashree Das, Pranati Patnaik, Jyotirmaya Mohanty, Gargee Mohanty","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136258445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nagula Harshith, Palleti Sai Yogesh, Kuttikattu Narayanan Kutty Bibin, Ramachandran Rabin, Ganesan Anusiya, Rengarajan Jai Ganesh
Pectin is a natural biopolymer with excellent biodegradability and biocompatibility that is often extracted from the peels of citrus fruits and apples. Pectin isolated from Citrus limon (L.) Burm peels is the primary nanofiber material in this investigation. Pectin: polyvinyl alcohol (PVA) ratio, solvent type, voltage, and flow rate were investigated to optimise the electrospinning procedure for producing pectin-based nanofibers. The peels of C. limon (L.) Burm were chemically extracted for their pectin, yielding a 20.5% yield, and subsequently electrospun with varying percentages of PVA. Sample 1, made from 10% pectin and 10% PVA, formed a mat with the best mechanical qualities out of the four samples. Despite being mixed with PVA, The Nanofiber’s Fourier transform infrared spectrum reveals that pectin retained its original chemical structure. Amorphous characteristics of the mat are shown in the X-ray diffractogram. Therapeutic pectin and PVA nanofibers could be made using PVA since they are cheap, biodegradable, and biocompatible.
{"title":"Synthesis of bio-nanofiber from pectin/polyvinyl alcohol for therapeutic application","authors":"Nagula Harshith, Palleti Sai Yogesh, Kuttikattu Narayanan Kutty Bibin, Ramachandran Rabin, Ganesan Anusiya, Rengarajan Jai Ganesh","doi":"10.7324/jabb.2023.91668","DOIUrl":"https://doi.org/10.7324/jabb.2023.91668","url":null,"abstract":"Pectin is a natural biopolymer with excellent biodegradability and biocompatibility that is often extracted from the peels of citrus fruits and apples. Pectin isolated from Citrus limon (L.) Burm peels is the primary nanofiber material in this investigation. Pectin: polyvinyl alcohol (PVA) ratio, solvent type, voltage, and flow rate were investigated to optimise the electrospinning procedure for producing pectin-based nanofibers. The peels of C. limon (L.) Burm were chemically extracted for their pectin, yielding a 20.5% yield, and subsequently electrospun with varying percentages of PVA. Sample 1, made from 10% pectin and 10% PVA, formed a mat with the best mechanical qualities out of the four samples. Despite being mixed with PVA, The Nanofiber’s Fourier transform infrared spectrum reveals that pectin retained its original chemical structure. Amorphous characteristics of the mat are shown in the X-ray diffractogram. Therapeutic pectin and PVA nanofibers could be made using PVA since they are cheap, biodegradable, and biocompatible.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135102771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Increasing demand and changes in pricing of fossil-derived fuel led researchers to find the productive path to meet the current energy requirement. These objectives can be achieved by using lignocellulosic wastes as a source of food for microbes having the ability to accumulate oil. Microorganisms possessing more than 20% w/w of lipids on dry weight are termed as oleaginous microorganisms. Oleaginous microorganisms are proven useful in the production of biodiesel. Coastal water was screened for the presence of these microorganisms. Potent lipid accumulator has been isolated from the selected sample. After studying morphology, biochemical, and 16s rRNA sequencing analysis, the organism was identified as Bacillus paralicheniformis (OQ202112). They can produce polyunsaturated fatty acids (PUFAs) de novo using PUFA synthase/polyketide synthase. The fatty acid profile revealed the presence of oleic and linoleic acids as major components. They produced 3.3 g/L lipids with 5.5 g/L total dry weight. Lipid extraction was carried out by Bligh and Dyer method and oil was transesterified by methanol using NaOH as a catalyst. The yield of fatty acid methyl esters was 86.2%. The fuel obtained met with the standards given by the American Society for Testing and Materials and ASAM. Cetane rating 61 suggests good quality of the biodiesel produced. Thus, the isolated organism possesses potential to be used as a biodiesel producer.
{"title":"Bacillus paralicheniformis (OQ202112) - Mediated biodiesel production using groundnut husk: A sustainable approach for bioenergy generation","authors":"Roshani Kantibhai Chaudhary, Priti Hemant Patel, Nikita Rajendrasinh Chavda, Vaidehi Kamlesh Patel, Kaushal Kantilal Chaudhary","doi":"10.7324/jabb.2023.157806","DOIUrl":"https://doi.org/10.7324/jabb.2023.157806","url":null,"abstract":"Increasing demand and changes in pricing of fossil-derived fuel led researchers to find the productive path to meet the current energy requirement. These objectives can be achieved by using lignocellulosic wastes as a source of food for microbes having the ability to accumulate oil. Microorganisms possessing more than 20% w/w of lipids on dry weight are termed as oleaginous microorganisms. Oleaginous microorganisms are proven useful in the production of biodiesel. Coastal water was screened for the presence of these microorganisms. Potent lipid accumulator has been isolated from the selected sample. After studying morphology, biochemical, and 16s rRNA sequencing analysis, the organism was identified as Bacillus paralicheniformis (OQ202112). They can produce polyunsaturated fatty acids (PUFAs) de novo using PUFA synthase/polyketide synthase. The fatty acid profile revealed the presence of oleic and linoleic acids as major components. They produced 3.3 g/L lipids with 5.5 g/L total dry weight. Lipid extraction was carried out by Bligh and Dyer method and oil was transesterified by methanol using NaOH as a catalyst. The yield of fatty acid methyl esters was 86.2%. The fuel obtained met with the standards given by the American Society for Testing and Materials and ASAM. Cetane rating 61 suggests good quality of the biodiesel produced. Thus, the isolated organism possesses potential to be used as a biodiesel producer.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":"298 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135699234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.7324/jabb.2023.139674
Palash Ghorai, Dipankar Ghosh
Agrobacterium rhizogenes as molecular tool for the production of hairy roots in Withania somnifera Manali Singh,, Deep Chandra Suyal, Nisha Dinkar, Soniya Joshi, Nishtha Srivastava, Vineet Kumar Maurya, Abhiruchi Agnihotri, Sanjeev Agrawal
{"title":"Screening and isolation of potential nitrogen-fixing Enterobacter sp. GG1 from mangrove soil with its accelerated impact on green chili plant (Capsicum frutescens L.) growth amelioration","authors":"Palash Ghorai, Dipankar Ghosh","doi":"10.7324/jabb.2023.139674","DOIUrl":"https://doi.org/10.7324/jabb.2023.139674","url":null,"abstract":"Agrobacterium rhizogenes as molecular tool for the production of hairy roots in Withania somnifera Manali Singh,, Deep Chandra Suyal, Nisha Dinkar, Soniya Joshi, Nishtha Srivastava, Vineet Kumar Maurya, Abhiruchi Agnihotri, Sanjeev Agrawal","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":"91 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135102277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nguyen Quang Duc Tien, Hoang Kha, Tran Linh Anh, Le Quang Man, Duong Tan Nhut, Nguyen Hoang Loc
Atherosclerotic plaque regression and HMG-CoA reductase inhibition potential of curcumin: An integrative omics and in-vivo studyPriyanka Riyad, Ashok Purohit, Karishma Sen, Heera Ram
姜黄素的动脉粥样硬化斑块消退和HMG-CoA还原酶抑制潜力:一项综合组学和体内研究
{"title":"3-Hydroxy-3-methylglutaryl-coenzyme A reductase of Vietnamese ginseng: Structure, promoter identification, and expression analysis","authors":"Nguyen Quang Duc Tien, Hoang Kha, Tran Linh Anh, Le Quang Man, Duong Tan Nhut, Nguyen Hoang Loc","doi":"10.7324/jabb.2023.11517","DOIUrl":"https://doi.org/10.7324/jabb.2023.11517","url":null,"abstract":"Atherosclerotic plaque regression and HMG-CoA reductase inhibition potential of curcumin: An integrative omics and in-vivo studyPriyanka Riyad, Ashok Purohit, Karishma Sen, Heera Ram","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136028349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.7324/jabb.2021.100102
R. Ritu, R. Jitender, K. Poonam, Singh Nater Pal, Santal Anita Rani
Plastics are synthetic polymers, which are frequently used in daily life for a wide range of purposes. These plastic wastes are accumulated and generate plastic pollution in the environment. It needs many years for complete deterioration in the environmental conditions. Biodegradation is the most promising method to treat plastic pollution, as the microorganisms utilize the low-density polyethylene (LDPE) as a sole source of carbon, and this indicates an innovative approach to manage the problem of plastic waste. The isolate SARR1 was identified as Bacillus licheniformis using the National Center for Biotechnology Information (NCBI) database. The isolate SARR1 showed an LDPE removal rate (K) of 0.069 g day−1 with a half-life of approximately 335.32 days to degrade LDPE strips. The biomass production was 0.98 ± 0.006 gl−1 (Xm) during the incubation of 30 days, and the percentage of crystallinity was significantly decreased from 71.69% to 50.78% due to biodegradation. The esterase and lipase activity of isolate SARR1 was studied using UV visible spectroscopy. The gas chromatography-mass spectrometry analysis confirmed the synthesis of acetone, diazene dimethyl, and carbamimidothioic acid, 1-methylethyl ester with different peak area percentages of 23.38%, 65.58%, and 11.04%, respectively. Seed germination study showed that the compounds formed after biodegradation of LDPE by bacterial strain SARR1 were eco-friendly.
{"title":"Biodegradation and detoxification of low-density polyethylene by an indigenous strain Bacillus licheniformis SARR1","authors":"R. Ritu, R. Jitender, K. Poonam, Singh Nater Pal, Santal Anita Rani","doi":"10.7324/jabb.2021.100102","DOIUrl":"https://doi.org/10.7324/jabb.2021.100102","url":null,"abstract":"Plastics are synthetic polymers, which are frequently used in daily life for a wide range of purposes. These plastic wastes are accumulated and generate plastic pollution in the environment. It needs many years for complete deterioration in the environmental conditions. Biodegradation is the most promising method to treat plastic pollution, as the microorganisms utilize the low-density polyethylene (LDPE) as a sole source of carbon, and this indicates an innovative approach to manage the problem of plastic waste. The isolate SARR1 was identified as Bacillus licheniformis using the National Center for Biotechnology Information (NCBI) database. The isolate SARR1 showed an LDPE removal rate (K) of 0.069 g day−1 with a half-life of approximately 335.32 days to degrade LDPE strips. The biomass production was 0.98 ± 0.006 gl−1 (Xm) during the incubation of 30 days, and the percentage of crystallinity was significantly decreased from 71.69% to 50.78% due to biodegradation. The esterase and lipase activity of isolate SARR1 was studied using UV visible spectroscopy. The gas chromatography-mass spectrometry analysis confirmed the synthesis of acetone, diazene dimethyl, and carbamimidothioic acid, 1-methylethyl ester with different peak area percentages of 23.38%, 65.58%, and 11.04%, respectively. Seed germination study showed that the compounds formed after biodegradation of LDPE by bacterial strain SARR1 were eco-friendly.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71216894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-15DOI: 10.7324/jabb.2022.100209
Jain Deepika
Bacterial corneal ulcer (BCU) is an infection that results into loss of vision (approx. 10%) and blindness. BCU requires constant attention since antibiotic treatment failure due to genetic alteration is common in microorganisms but the present study confirms that the ineffective treatment of corneal blindness is due to this novel bacterium. Around 300 corneal scrapings were performed in patients presenting with corneal ulcers from ophthalmology hospitals, government hospitals, and clinical laboratories of different places in Maharashtra, India. The novel pathogenic bacterium, i.e., Pseudomonas gessardii, was isolated from corneal ulcer samples from patients and identified using molecular characterization. The most common risk factors associated with BCU are contact lens and trauma. The pathogen produces a pyoverdine pigment, nitric oxide scavenger, exhibiting antioxidant activity. The novel bacterium is sensitive to the fluoroquinolones group of antibiotics and tobramycin. It is necessary to diagnose corneal ulcers at an early stage to reduce the probability of permanent loss of eye sight and corneal structural damage.
{"title":"Pseudomonas gessardii—A novel pathogenic bacterium associated with the cases of corneal ulcers and producing virulent pyoverdine pigment","authors":"Jain Deepika","doi":"10.7324/jabb.2022.100209","DOIUrl":"https://doi.org/10.7324/jabb.2022.100209","url":null,"abstract":"Bacterial corneal ulcer (BCU) is an infection that results into loss of vision (approx. 10%) and blindness. BCU requires constant attention since antibiotic treatment failure due to genetic alteration is common in microorganisms but the present study confirms that the ineffective treatment of corneal blindness is due to this novel bacterium. Around 300 corneal scrapings were performed in patients presenting with corneal ulcers from ophthalmology hospitals, government hospitals, and clinical laboratories of different places in Maharashtra, India. The novel pathogenic bacterium, i.e., Pseudomonas gessardii, was isolated from corneal ulcer samples from patients and identified using molecular characterization. The most common risk factors associated with BCU are contact lens and trauma. The pathogen produces a pyoverdine pigment, nitric oxide scavenger, exhibiting antioxidant activity. The novel bacterium is sensitive to the fluoroquinolones group of antibiotics and tobramycin. It is necessary to diagnose corneal ulcers at an early stage to reduce the probability of permanent loss of eye sight and corneal structural damage.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77953704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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