Isolation of antifungal bioactive compounds from an ethanolic extract of beluntas leaves (Pluchea indica) against Microsporum canis

Devi Safitri, M. Muhaimin, Nelson Nelson, I. L. Tarigan, L. Lizawati, M. Latief
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Abstract

Background: Fungus prevalence in Indonesia is still relatively high. This happens because Indonesia has high temperatures and humidity. Beluntas (Pluchea indica) is a shrub native to Indonesia and widely distributed in most parts of the country. Pluchea indica is usually used by the community as a medicine for skin diseases. The bioactive content of P. indica leaves has potential as an antifungal agent. One of the fungi that is pathogenic to humans is Microsporum canis. Method: N-hexane, ethyl acetate, and ethanol served as the solvents for the graded maceration method of extraction over two 24-hour periods. Furthermore, using Vacuum Liquid Chromatography (VLC) and characterization with UV-Vis and FT-IR. Antifungal activity was performed by disc diffusion method. Results: Pluchea indica leaves have antifungal activity in the ethanol extract of 20.08 mm (very strong), the F5 fraction of 22.24 mm (very strong), and the F5 isolate of 6.3 mm (weak) at a concentration of 4%. Based on the UV-Vis and FT-IR data, the isolate had a wavelength of 267 nm with a functional group of -OH, C=C aromatic, C-H, and C-O. Conclusion: Isolate from the ethanol extract of Pluchea indica leaves has potential as an antifungal originating from the flavonoid group. Keywords: Pluchea indica, antifungal, Microsporum canis, isolation compound
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白桦叶乙醇提取物对犬小孢子虫抗真菌活性化合物的分离
背景:真菌在印度尼西亚的流行率仍然相对较高。这是因为印尼的高温和高湿度。白龙塔(Pluchea indica)是一种原产于印度尼西亚的灌木,广泛分布于该国的大部分地区。梅花通常被社会用作治疗皮肤病的药物。籼稻叶片的生物活性成分具有作为抗真菌剂的潜力。对人类有致病性的真菌之一是犬小孢子菌。方法:以正己烷、乙酸乙酯和乙醇为溶剂,采用梯度浸渍法提取,时间为2小时。此外,利用真空液相色谱(VLC)和紫外可见光谱(UV-Vis)和红外光谱(FT-IR)进行表征。用圆盘扩散法测定其抗真菌活性。结果:在浓度为4%时,梅子叶乙醇提取物20.08 mm(很强)、F5提取物22.24 mm(很强)、F5分离物6.3 mm(弱)均有抗真菌活性。通过UV-Vis和FT-IR分析,该分离物的波长为267 nm,官能团为-OH、C=C芳、C- h和C- o。结论:从梅子叶乙醇提取物中分离得到的一种黄酮类化合物具有抗真菌活性。关键词:梅花,抗真菌,犬小孢子菌,分离化合物
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Pengetahuan, sikap, dan perilaku konsumsi suplemen dan obat tradisional mahasiswa Universitas Jember saat pandemi COVID-19 Hubungan Tingkat Pengetahuan dengan Perilaku Swamedikasi Obat Golongan NSAID di Apotek X Banjarmasin EFEKTIVITAS KOMBINASI EKSTRAK ETANOL 70% DAUN RAMANIA (Bouea macrophylla Griffith) dan GLIBENKLAMID DENGAN METODE INTOLERANSI GLUKOSA PERBANDINGAN KADAR GLUKOSA DARAH DAN HBA1C PADA INSULIN GLARGINE DAN DETEMIR PASIEN DIABETES MELITUS TYPE 2 DI RUMAH SAKIT UMUM DAERAH ULIN BANJARMASIN The study of conformity of the pharmaceutical service in Puskesmas Bogor City towards PMK No. 74, 2016
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