First report of chromosome and karyological analysis of Gekko nutaphandi (Gekkonidae, Squamata) from Thailand: Neo-diploid chromosome number in genus Gekko

IF 2.1 4区 生物学 Q2 Agricultural and Biological Sciences Caryologia Pub Date : 2023-04-28 DOI:10.36253/caryologia-1875
Weera Thongnetr, S. Prasopsin, S. Aiumsumang, Sukhonthip Ditcharoen, A. Tanomtong, Prayoon Wongchantra, Wutthisak Bunnaen, S. Phimphan
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Abstract

The karyotypes of red-eyed Gecko are not reported yet. Herein, we describe the karyotypes of red-eyed Gecko (Gekko nutaphandi Bauer, Sumontha & Pauwels, 2008) from Thailand. Gecko chromosome preparations were directly conducted from bone marrow and testis. Chromosomal characteristics were analyzed by Giemsa staining, Ag-NOR banding as well as fluorescence in situ hybridization (FISH) using microsatellites d(GC)15 probe. The results showed that the number of diploid chromosomes is 2n=34, while the fundamental number (NF) is 46 in both males and females. The types of chromosomes were 4 large metacentric, 6 large submetacentric, 2 medium telocentric, 2 small metacentric and 20 small telocentric chromosomes. The results of conventional Giemsa staining presented the diploid chromosome number differentiation even in the same genus. NORs are located at the secondary constriction to the telomere on the long arm of chromosome pair 5. There are no sex differences in karyotypes between males and females. FISH with d(GC)15 sequences were also displayed at the telomeres of most other chromosomes. We found that during metaphase I the homologous chromosomes showed synapsis, which can be defined as 19 ring bivalents and 17 haploid chromosomes (n=17) at metaphase II as a diploid species. The karyotype formula is as follows: 2n (34) = L4m+L6sm+M2t+S2m+S20t.
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泰国斑壁虎染色体及核学分析首次报道:斑壁虎属新二倍体染色体数目
红眼壁虎的核型尚未见报道。在此,我们描述了来自泰国的红眼壁虎(Gekko nutaphandi Bauer, Sumontha & Pauwels, 2008)的核型。直接从骨髓和睾丸制备壁虎染色体。采用Giemsa染色、Ag-NOR显带和微卫星d(GC)15探针荧光原位杂交(FISH)分析染色体特征。结果表明,雄性和雌性的二倍体染色体数目为2n=34,而基本数目(NF)为46。染色体类型为4条大稳中心、6条大亚稳中心、2条中远中心、2条小稳中心和20条小远中心染色体。常规吉氏染色结果显示,即使在同一属中,二倍体染色体数目也有分化。NORs位于第5对染色体长臂端粒的二次缩窄处。男性和女性的核型没有性别差异。具有d(GC)15序列的FISH也显示在大多数其他染色体的端粒上。我们发现在中期I,同源染色体出现突触,可以定义为19个环二价染色体和17个单倍体染色体(n=17),作为一个二倍体物种。核型公式为:2n (34) = L4m+L6sm+M2t+S2m+S20t。
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来源期刊
Caryologia
Caryologia 生物-遗传学
CiteScore
1.60
自引率
23.80%
发文量
26
审稿时长
12 months
期刊介绍: Caryologia is devoted to the publication of original papers, and occasionally of reviews, about plant, animal and human karyological, cytological, cytogenetic, embryological and ultrastructural studies. Articles about the structure, the organization and the biological events relating to DNA and chromatin organization in eukaryotic cells are considered. Caryologia has a strong tradition in plant and animal cytosystematics and in cytotoxicology. Bioinformatics articles may be considered, but only if they have an emphasis on the relationship between the nucleus and cytoplasm and/or the structural organization of the eukaryotic cell.
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