Endophytic Fungi Isolated from Musa acuminata 'Dwarf Cavendish' and their Activity against Phytopathogenic Fungi

Journal of Agriculture Biotechnology Pub Date : 2016-08-02 DOI:10.20936/JAB/160107

A. Cosoveanu, E. Martin, C. Mariño, M. Reina, Rhiannon Miriam Flavin, R. Cabrera

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引用次数: 5

Abstract

Endophytic fungi were isolated from Musa acuminata ‘Dwarf Cavendish’ harvested from 100-year-old crops. The antagonistic bioactivity and the inhibitory activity of the endophytic fungi were evaluated against various phytopathogenic fungi: Fusarium oxysporum f. sp. lycopersici (Sacc.) Snyder y Hansen, F. oxysporum Schlect. f.sp. cubense (E.F. Smith) Snyd. & Hans, F. moniliforme (Sheldon), Alternaria alternata (Fr.) Keissl, Geotrichum sp, Phoma sp. and Cladosporium sp. In dual culture assays, endophytic strains of Penicillium, Aspergillus, Fusarium and Chaetomium were found to be active against some of the targeted microorganisms. Four active extracts were fractionised using VLC (vacuum liquid chromatography) and two ethyl acetate and ethanolic extracts obtained from Aspergillus oryzae isolate led to two active hexanic and ethyl acetate fractions which inhibited growth of F. moniliforme (%I = 48.02 and 55.1, respectively; at 1 mg ml?1). The smallest EC50 (0.74 mg ml?1) was observed in an extract obtained from a strain of Penicillium sp. The same endophytic strain inhibited F. oxysporum by 59.72% in VOC (volatile organic compounds) assays.

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矮卡文迪什Musa acuminata 'Dwarf Cavendish'内生真菌分离及其抗植物病原真菌活性

从100年作物中收获的Musa acuminata ' Dwarf Cavendish '中分离出内生真菌。测定了内生真菌对多种植物病原真菌的拮抗活性和抑制活性:镰刀菌(Fusarium oxysporum f. sp. lycopersici, Sacc.)。施耐德·汉森，F. oxysporum Schlect。f.sp。立方体(E.F.史密斯)& Hans, F. moniliformme (Sheldon)， alternnaria alternata (Fr.) Keissl, Geotrichum sp, Phoma sp和Cladosporium sp.在双重培养试验中，发现内生菌株青霉菌，曲霉，镰刀菌和毛毛菌对一些目标微生物有活性。利用VLC(真空液相色谱法)对4种活性提取物进行分离，从米曲霉分离物中得到2种乙酸乙酯和乙醇提取物，得到抑制moniliforme生长的己烷和乙酸乙酯活性组分(%I分别为48.02和55.1);1 mg ml?1)。从一株青霉菌提取的提取物中观察到最小的EC50 (0.74 mg ml?1)，同一株内生菌株在VOC(挥发性有机物)测定中对尖孢镰刀菌的抑制率为59.72%。

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Journal of Agriculture Biotechnology

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