A. Cosoveanu, E. Martin, C. Mariño, M. Reina, Rhiannon Miriam Flavin, R. Cabrera
Endophytic fungi were isolated from Musa acuminata ‘Dwarf Cavendish’ harvested from 100-year-old crops. The antagonistic bioactivity and the inhibitory activity of the endophytic fungi were evaluated against various phytopathogenic fungi: Fusarium oxysporum f. sp. lycopersici (Sacc.) Snyder y Hansen, F. oxysporum Schlect. f.sp. cubense (E.F. Smith) Snyd. & Hans, F. moniliforme (Sheldon), Alternaria alternata (Fr.) Keissl, Geotrichum sp, Phoma sp. and Cladosporium sp. In dual culture assays, endophytic strains of Penicillium, Aspergillus, Fusarium and Chaetomium were found to be active against some of the targeted microorganisms. Four active extracts were fractionised using VLC (vacuum liquid chromatography) and two ethyl acetate and ethanolic extracts obtained from Aspergillus oryzae isolate led to two active hexanic and ethyl acetate fractions which inhibited growth of F. moniliforme (%I = 48.02 and 55.1, respectively; at 1 mg ml?1). The smallest EC50 (0.74 mg ml?1) was observed in an extract obtained from a strain of Penicillium sp. The same endophytic strain inhibited F. oxysporum by 59.72% in VOC (volatile organic compounds) assays.
{"title":"Endophytic Fungi Isolated from Musa acuminata 'Dwarf Cavendish' and their Activity against Phytopathogenic Fungi","authors":"A. Cosoveanu, E. Martin, C. Mariño, M. Reina, Rhiannon Miriam Flavin, R. Cabrera","doi":"10.20936/JAB/160107","DOIUrl":"https://doi.org/10.20936/JAB/160107","url":null,"abstract":"Endophytic fungi were isolated from Musa acuminata ‘Dwarf Cavendish’ harvested from 100-year-old crops. The antagonistic bioactivity and the inhibitory activity of the endophytic fungi were evaluated against various phytopathogenic fungi: Fusarium oxysporum f. sp. lycopersici (Sacc.) Snyder y Hansen, F. oxysporum Schlect. f.sp. cubense (E.F. Smith) Snyd. & Hans, F. moniliforme (Sheldon), Alternaria alternata (Fr.) Keissl, Geotrichum sp, Phoma sp. and Cladosporium sp. In dual culture assays, endophytic strains of Penicillium, Aspergillus, Fusarium and Chaetomium were found to be active against some of the targeted microorganisms. Four active extracts were fractionised using VLC (vacuum liquid chromatography) and two ethyl acetate and ethanolic extracts obtained from Aspergillus oryzae isolate led to two active hexanic and ethyl acetate fractions which inhibited growth of F. moniliforme (%I = 48.02 and 55.1, respectively; at 1 mg ml?1). The smallest EC50 (0.74 mg ml?1) was observed in an extract obtained from a strain of Penicillium sp. The same endophytic strain inhibited F. oxysporum by 59.72% in VOC (volatile organic compounds) assays.","PeriodicalId":14885,"journal":{"name":"Journal of Agriculture Biotechnology","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82327172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-07-15DOI: 10.22353/mjbs.2016.14.01
N. Amartuvshin, Nanjidsuren Ochgerel
Maximum of mean seed weight was observed at 1997 while normalised seed weight was significantly higher at 1982 and 1995, compared with other years. Seed weight variation is positively correlated with mean seed weight for earlier rainy season years, excluding later rainy season years (1982 and 1995). Low and high seed weight variation associated for years with colder and wetter in May and with hotter and drier in May, suggesting drought in May might be effective on seed weight variation of I. dichotoma. Moderate normalised seed weight associated for years with hotter in June while high and low normalised seed weight for years with cooler and cool in June, suggesting I. dichotoma might use both photosynthetic productions and underground-storage for seed maturity. Mean seed weight and seed weight variation were linked to precipitation amount whereas normalized seed weight was linked to precipitation periodicity. Seed weight variation detected delay of regrowth, caused by early drought while normalized seed weight suggested seed weight response to complete seed maturation after droughty season, using photosynthetic productions and underground-storage. The results suggest that normalized seed weight might be useful to recognize seed weight response of I. dichotoma for climatic factors, better than mean seed weight and seed weight variation.
{"title":"Yearly Difference in Normalised Seed Weight of Cultivated Iris dichotoma Pall in Mongolia","authors":"N. Amartuvshin, Nanjidsuren Ochgerel","doi":"10.22353/mjbs.2016.14.01","DOIUrl":"https://doi.org/10.22353/mjbs.2016.14.01","url":null,"abstract":"Maximum of mean seed weight was observed at 1997 while normalised seed weight was significantly higher at 1982 and 1995, compared with other years. Seed weight variation is positively correlated with mean seed weight for earlier rainy season years, excluding later rainy season years (1982 and 1995). Low and high seed weight variation associated for years with colder and wetter in May and with hotter and drier in May, suggesting drought in May might be effective on seed weight variation of I. dichotoma. Moderate normalised seed weight associated for years with hotter in June while high and low normalised seed weight for years with cooler and cool in June, suggesting I. dichotoma might use both photosynthetic productions and underground-storage for seed maturity. Mean seed weight and seed weight variation were linked to precipitation amount whereas normalized seed weight was linked to precipitation periodicity. Seed weight variation detected delay of regrowth, caused by early drought while normalized seed weight suggested seed weight response to complete seed maturation after droughty season, using photosynthetic productions and underground-storage. The results suggest that normalized seed weight might be useful to recognize seed weight response of I. dichotoma for climatic factors, better than mean seed weight and seed weight variation.","PeriodicalId":14885,"journal":{"name":"Journal of Agriculture Biotechnology","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78369727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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