{"title":"Nonlinear Spectral Imaging Microscopy","authors":"J. Palero","doi":"10.1002/IMIC.200990008","DOIUrl":null,"url":null,"abstract":"The deep tissue penetration of nonlinear microscopy and the high detection efficiency of a spectrograph are utilized to record spectral images of the intrinsic emission of living mouse skin tissues. Visualization of the spectral images by wavelength-to-RGB color conversion allowed identification and discrimination of tissue structures Nonlinear spectral imaging microscopy (NSIM) can provide a wealth of information not easily obtainable with present conventional nonlinear imaging systems.","PeriodicalId":100658,"journal":{"name":"Imaging & Microscopy","volume":"65 1","pages":"22-25"},"PeriodicalIF":0.0000,"publicationDate":"2009-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Imaging & Microscopy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/IMIC.200990008","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
The deep tissue penetration of nonlinear microscopy and the high detection efficiency of a spectrograph are utilized to record spectral images of the intrinsic emission of living mouse skin tissues. Visualization of the spectral images by wavelength-to-RGB color conversion allowed identification and discrimination of tissue structures Nonlinear spectral imaging microscopy (NSIM) can provide a wealth of information not easily obtainable with present conventional nonlinear imaging systems.