Association of gene polymorphisms of angiotensin metabolism with left ventricular diastolic dysfunction in hypertensive patients

Abstract

Aim. To investigate the relationship of SNP ACE ((AluIns/DelI>D), rs 4646994), SNP of angiotensinogen 1 gene AGT :521 C>T ((Thr 174 Met), rs 4762), SNP of angiotensinogen 2 gene AGT:704 T>C ((Met 235 Thr), rs699), SNP of angiotensin-2 type 1 receptor gene AGTR1: A1166C; A>C (rs5186) with left ventricular diastolic dysfunction in hypertensive patients.Methods and materials. 74 patients (26 women and 48 men) with stage 1-2 hypertension were examined. The analysis of genetic polymorphisms was carried out by means of polymerase chain reaction method with the analysis of DNA RFLP (restriction fragment length polymorphism).Results. CC polymorphisms of genotype AGT:704 T>C (Met 235 Thr), (χ2 = 8.18; p = 0.017) were detected in the group of patients with left ventricular diastolic dysfunction, which nearly triples the probability of impaired myocardial relaxation (OR 2.85) and CC polymorphisms of genotype of angiotensinogen type 1 receptor gene (AGTR1: A1166C), (χ2 = 1.77; p = 0.041), which doubles the probability of left ventricular diastolic dysfunction (OR 2.39).Conclusion. CC genotype AGT:704 T>C (Met 235 Thr) and CC genotype of the angiotensinogen type 1 receptor gene (AGTR1: A1166C) are associated with the development of left ventricular diastolic dysfunction in patients with hypertension.