Pengaruh Pemberian Kopi yang Diformulasikan dengan Antioksidan dan Gula Kelapa terhadap Tekanan Darah, MDA, dan SOD Serum Tikus Obesitas

Abstract

Development of coffee mix rich in antioxidant by adding coconut sugar and red palm oil (RPO) is an alternative to supplying antioxidants in obese individuals associated with increased oxidative stress. The aim of this research is to determine the effect of feeding coffee mixed with coconut sugar on SOD, MDA, and blood pressure in obese rats. This was an experimental study which involved 18 rats that were induced with a high-fat diet into obese rats (Lee index> 0.3). These rats were divided into three groups (n=6) and each was given treatment as follows: 1) coffee mixed with non RPO with cane sugar (0.45 g) / day (control) (P1); 2) coffee mixed with coconut sugar with RPO (0.45 g /day (P2); and 3) coffee mixed with coconut sugar with RPO (0.90 g / day (P3). The intervention was carried out for two weeks. Changes in body weight were observed every week, and the data of blood pressure, SOD levels, and serum MDA levels were observed pre and post treatment. The result showed that feeding coffee mixed with coconut sugar with RPO to obese mice (P2 and P3) significantly decreased blood pressure (26.9% and 40.6%) and serum MDA levels (35.3% and 61.8%), and increased serum SOD levels (28.79% and 53.66%) respectively, but the group given coffee mixed with coconut sugar with RPO, the blood pressure and MDA levels increased (2.9% and 1.9%), while the SOD levels decreased (16.6%). The change in the body weight was higher in P1 (7.8%), compared with the group given coffee mixed with coconut sugar (P2 = 6.7%; P3 = 4.4%). Coffee mix that is rich in antioxidant from red palm oil and coconut sugar potentially becomes a functional drink to reduce oxidative stress in obese rats.