Establishment of primary 3D cell culture based on magnetic bioprinting for colorectal cancer cells from patients in Cipto Mangunkusumo National Hospital Indonesia

M. Abdullah, B. Bela, A. Syam, M. Simadibrata, Sofy Meilany, Firda Annisa, Dian Amirulloh, D. Makmun, A. A. Rani
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引用次数: 4

Abstract

Colorectal cancer is the third most common malignancy with more than 1.2 million new annual cases in the world. Methods for propagating cancer cells range from conventional to the more advanced system. Cancer cells are routinely cultured in two-dimensional (2D) model. This method faces several limitations attributable to lack of structural architecture and nature condition. Three-dimensional (3D) model mimics in vivo environment more closely than the conventional 2D. This strategy may help in elucidating cancer biology and subsequently contribute to higher success of therapy. The goal of this study was to establish the primary 3D culture from colorectal cancer collected from patients in Cipto Mangunkusumo National Hospital. Cancer cells were extracted mechanically and grown in DMEM/F12 medium supplemented with serum and antibiotics. The NanoShuttle-PL was added to the cells and magnetic drive was used to form cell spheroids. There were 22 samples obtained with varying cancer stages viz. IIA, IIIB, IIIC, and IV. Primary culture of cancer cells successfully established by mechanically extracting the tumour tissues into small pieces. Results on magnetic bioprinting revealed that the 3D culture (spheroids) was successfully established rapidly. Further studies are needed to evaluate culture viability and cell compositions, as well as performing the chemosensitivity testing.
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基于磁性生物打印的印尼Cipto Mangunkusumo国立医院结直肠癌细胞原代3D细胞培养的建立
结直肠癌是世界上第三大最常见的恶性肿瘤,每年有120多万新病例。增殖癌细胞的方法从传统的到更先进的系统都有。癌细胞通常在二维(2D)模型中培养。由于结构结构和自然条件的限制,这种方法存在一定的局限性。三维(3D)模型比传统的二维模型更能模拟体内环境。这一策略可能有助于阐明癌症生物学,并随后有助于更高的治疗成功率。本研究的目的是建立从Cipto Mangunkusumo国立医院收集的结直肠癌患者的初代3D培养。机械提取癌细胞,在添加血清和抗生素的DMEM/F12培养基中生长。将NanoShuttle-PL添加到细胞中,并使用磁驱动形成细胞球体。共获得22个不同分期的样本,即IIA, IIIB, IIIC和IV。通过机械提取肿瘤组织成小块,成功建立了癌细胞的原代培养。磁性生物打印的结果表明,3D培养(球体)快速建立成功。需要进一步的研究来评估培养活力和细胞组成,以及进行化学敏感性测试。
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