PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations最新文献

{"title":"Ultrasound-assisted extraction of polyphenols from pineapple skin","authors":"Nur Azzanizawaty Yahya, R. A. Wahab, Therese Lim Shuh Xine, M. Hamid","doi":"10.1063/1.5125506","DOIUrl":"https://doi.org/10.1063/1.5125506","url":null,"abstract":"Pineapple skin (PS) is among the major food wastes from canning industries which contributes to environmental pollution. Nonetheless, PS contains an appreciable quantity of valuable phenolic compounds that may have potential applications in pharmaceutical and cosmetics industries. Since much of the PS waste are discarded, recovery of this agro-industrial biomass for possible transformations into value-added products merit scientific attention. In this study, phenolic compounds were recovered from PS using a greener method of ultrasonic-assisted extraction (UAE). The study aimed to assess the effect of sonication time to extract the highest concentration of polyphenolics from the PP waste. It was demonstrated that the highest extraction yield (29.04 %) was obtained at 6.49 min sonication time, whereas maximum total phenolic content (TPC) and total flavonoid content (TFC) of 1078.68 ± 1.32 mg GAE/g DW and 1276.64 ± 5.92 mg QE/g DW, respectively, were attained following sonication at 5.92 min. These values seen here were considerably higher than that obtained using Soxhlet reflux extraction (24.95% and 7.98 mg GAE/g DW) and drying process (4.16 ± 0.24 mg GAE/100g DW and 76.93 ± 11.85 µg rutin equivalent/100g DW). The findings, therefore, envisioned the efficacious UAE method for a greener and higher recovery of polyphenolic compounds from PS waste.Pineapple skin (PS) is among the major food wastes from canning industries which contributes to environmental pollution. Nonetheless, PS contains an appreciable quantity of valuable phenolic compounds that may have potential applications in pharmaceutical and cosmetics industries. Since much of the PS waste are discarded, recovery of this agro-industrial biomass for possible transformations into value-added products merit scientific attention. In this study, phenolic compounds were recovered from PS using a greener method of ultrasonic-assisted extraction (UAE). The study aimed to assess the effect of sonication time to extract the highest concentration of polyphenolics from the PP waste. It was demonstrated that the highest extraction yield (29.04 %) was obtained at 6.49 min sonication time, whereas maximum total phenolic content (TPC) and total flavonoid content (TFC) of 1078.68 ± 1.32 mg GAE/g DW and 1276.64 ± 5.92 mg QE/g DW, respectively, were attained following sonication at 5.92 min. These values s...","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77122293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Callogenesis of Durio zibethinus using flower bud explant","authors":"Y. Anggraito, N. Hermayani, M. Abdullah, N. A. Habibah, A. Retnoningsih","doi":"10.1063/1.5125543","DOIUrl":"https://doi.org/10.1063/1.5125543","url":null,"abstract":"Propagation of superior durian (Durio zibethinus) are constrained due to the limited number of the tree. Grafting and budding are commonly used in durian propagation, however these techniques give destructive effect to mother plant. Alternatively, in vitro technique can be chosen for propagation of superior durian. The research aims to study the possibility of durian flower bud as an explant for micropropagation because this part is relatively more sterile than other organs. Half strength Murashige dan Skoog media were used to grow explant with addition of 1 ppm 2,4-dichlorophenoxyacetic acid (2,4-D) and various combinations of sucrose concentration (30 gr/L, 50 gr/L and 70 gr/L), and Thidiazuron (TDZ, 0.2 ppm, 0.4 ppm and 0.8 ppm). Totally, there were nine treatment combinations with six replications, respectively. Parameters observed were number of explants-producing callus, colour and callus morphology. The result indicated that flower bud of durian had a potential as an explant for micropropagation due to their ability to induce callogenesis (69.2%). Combination of 45 gr/L sucrose and 0.8 ppm TDZ gave the best result in inducing callus growth. Most of the callus showed white colour and friable callus.Propagation of superior durian (Durio zibethinus) are constrained due to the limited number of the tree. Grafting and budding are commonly used in durian propagation, however these techniques give destructive effect to mother plant. Alternatively, in vitro technique can be chosen for propagation of superior durian. The research aims to study the possibility of durian flower bud as an explant for micropropagation because this part is relatively more sterile than other organs. Half strength Murashige dan Skoog media were used to grow explant with addition of 1 ppm 2,4-dichlorophenoxyacetic acid (2,4-D) and various combinations of sucrose concentration (30 gr/L, 50 gr/L and 70 gr/L), and Thidiazuron (TDZ, 0.2 ppm, 0.4 ppm and 0.8 ppm). Totally, there were nine treatment combinations with six replications, respectively. Parameters observed were number of explants-producing callus, colour and callus morphology. The result indicated that flower bud of durian had a potential as an explant for micropropagation du...","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86733460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proximate compositions of Ipomea aquatic Forsk. (leaf, petiole and stem) from Lubok Bungor, Jeli, Kelantan","authors":"A. Shariff, Norul Aqidah Shapiai, M. Zakaria, J. H. Akanda, N. Noor, M. Yusop, U. Razali","doi":"10.1063/1.5125564","DOIUrl":"https://doi.org/10.1063/1.5125564","url":null,"abstract":"Water spinach has been recognized as a rich source of minerals, bioactive compounds, vitamins and antioxidants. Its importance has attracted a lot of scientific research to assess their nutrients content. In this study, three parts of water spinach, namely: leaf, petiole and stem were examined. The objective of this study was to determine the content of crude protein, fat, carbohydrate, ash, fibre, moisture content and pH in leave, petiole and stem of water spinach. The differences of the nutritional content between the water spinach parts were then compared. Based on this study, stem contained the highest concentration of crude protein (4.36±0.05 g/100g), fibre (6.02±0.20 g/100g) which is significantly difference from the other two parts, and to some degree, fat (2.36±0.20 g/100g), even though with non-significance difference with petiole. High amounts of carbohydrate (7.53±0.92 g/100g) is recorded in the leaf, which is highly significant from the stem and the petiole and is incline towards alkalinity as indicated by the pH value (6.11±0.02 g/100g). The petiole is significantly rich in moisture (79.26±0.54 g/100g) compared to leaf & stem. Whilst, petiole has the highest percentage of crude ash 10.29±0.75 g/100g, which is significantly different from the leaf at 8.69±0.75g/100g but remains non significance to the stem at 9.75±0.37g/100g. This study showed that stem of water spinach has the most complete nutrients in the form of crude protein, fat and fibre compared to petiole and leaf, and is available all year round.Water spinach has been recognized as a rich source of minerals, bioactive compounds, vitamins and antioxidants. Its importance has attracted a lot of scientific research to assess their nutrients content. In this study, three parts of water spinach, namely: leaf, petiole and stem were examined. The objective of this study was to determine the content of crude protein, fat, carbohydrate, ash, fibre, moisture content and pH in leave, petiole and stem of water spinach. The differences of the nutritional content between the water spinach parts were then compared. Based on this study, stem contained the highest concentration of crude protein (4.36±0.05 g/100g), fibre (6.02±0.20 g/100g) which is significantly difference from the other two parts, and to some degree, fat (2.36±0.20 g/100g), even though with non-significance difference with petiole. High amounts of carbohydrate (7.53±0.92 g/100g) is recorded in the leaf, which is highly significant from the stem and the petiole and is incline towards alkalinity as...","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84841279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vermicomposting of landfill leachate using earthworms for biofertilizer production","authors":"S. Shaarani, Noor Zalina Hashim, Z. Arshad, R. C. Man, A. I. Nafsun, S. K. A. Mudalip, S. Z. Sulaiman","doi":"10.1063/1.5125524","DOIUrl":"https://doi.org/10.1063/1.5125524","url":null,"abstract":"The accumulation of waste in landfill site is mainly due to increased urbanization and industrialization. The leachate produced beneath the landfills led to critical environmental issue. The objective of this study was to determine the effects of vermicomposting of landfill leachate using earthworms on biofertilizer production. Earthworms were utilized to convert organic matter in leachate into plant-nutrients that enhance the growth of plants and plants productivity. The concentration of nitrate (N), phosphorus (P) and potassium(K) in vermicompost was found to increase while pH and number of earthworms declined at the end of the vermicomposting period. The vermibin containing 90 earthworms (V1) (approximate 52g) in a mixture of 150 ml of leachate and 798 g of soil obtained the highest concentration of NPK compared to those containing 39 (V2) earthworms and 15 (V3) earthworms. This indicates that the concentration of NPK increased with the added amount of earthworms. V1 experienced an increase of 53–fold in N, 194-fold in P and 210–fold in K due to earthworm activity during the vermicomposting, thus improving the number of leaves (32 leaves). The data shows that vermicomposting might be a suitable technology for the decomposition of landfill leachate into nutritive biofertilizer.The accumulation of waste in landfill site is mainly due to increased urbanization and industrialization. The leachate produced beneath the landfills led to critical environmental issue. The objective of this study was to determine the effects of vermicomposting of landfill leachate using earthworms on biofertilizer production. Earthworms were utilized to convert organic matter in leachate into plant-nutrients that enhance the growth of plants and plants productivity. The concentration of nitrate (N), phosphorus (P) and potassium(K) in vermicompost was found to increase while pH and number of earthworms declined at the end of the vermicomposting period. The vermibin containing 90 earthworms (V1) (approximate 52g) in a mixture of 150 ml of leachate and 798 g of soil obtained the highest concentration of NPK compared to those containing 39 (V2) earthworms and 15 (V3) earthworms. This indicates that the concentration of NPK increased with the added amount of earthworms. V1 experienced an increase of 53–fold ...","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84254303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preface: Proceedings of the 2nd International Conference on Biosciences and Medical Engineering (ICBME2019)","authors":"MahatNaji Arafat, WahabRoswanira Ab., HuyopFahrul Zaman, KeyonAemi Syazwani Abdul, AttanNursyafreena Binti, ChandrenSheela, GunamIda Bagus Wayan","doi":"10.1063/1.5125504","DOIUrl":"https://doi.org/10.1063/1.5125504","url":null,"abstract":"","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89926066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Towards establishing a non-destructive technique for forensic ink analysis involving Raman spectroscopy with chemometric procedures","authors":"Muhammad Naeim Mohamad Asri, Norhusnaton Najihah Yahya, N. A. M. Nor, W. N. S. M. Desa, D. Ismail","doi":"10.1063/1.5125510","DOIUrl":"https://doi.org/10.1063/1.5125510","url":null,"abstract":"Discrimination of ink is one of most challenging aspects in forensic science. Conversely, discrimination of inks had long been debated in the field, yet direct objective conclusion has been rarely achieved. Such a discrimination is nonetheless essential to provide explanations in term of variation in reproducibility or robustness. In this study, we demonstrated the capability of Raman spectroscopy (RS) coupled with Principal Component Analysis (PCA) for discrimination of gel pen inks. Based on the score plots of PC1 vs PC2, discrimination of 83.8% was achieved and 85.7% when PC1 vs PC2 vs PC3 were considered. As the application of chemometric techniques (PCA) to the forensic samples is a relatively new, but promising concept, further development for tasks relating to ink discrimination is recommended in this study for future work. Indeed, RS coupled with chemometrics would rapidly provide objective conclusion in forensic chemistry.Discrimination of ink is one of most challenging aspects in forensic science. Conversely, discrimination of inks had long been debated in the field, yet direct objective conclusion has been rarely achieved. Such a discrimination is nonetheless essential to provide explanations in term of variation in reproducibility or robustness. In this study, we demonstrated the capability of Raman spectroscopy (RS) coupled with Principal Component Analysis (PCA) for discrimination of gel pen inks. Based on the score plots of PC1 vs PC2, discrimination of 83.8% was achieved and 85.7% when PC1 vs PC2 vs PC3 were considered. As the application of chemometric techniques (PCA) to the forensic samples is a relatively new, but promising concept, further development for tasks relating to ink discrimination is recommended in this study for future work. Indeed, RS coupled with chemometrics would rapidly provide objective conclusion in forensic chemistry.","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78899507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of cocoa vinegar from pulp liquids fermentation by various methods","authors":"Gusti Putu Ganda-Putra, N. Wartini, L. Darmayanti","doi":"10.1063/1.5125542","DOIUrl":"https://doi.org/10.1063/1.5125542","url":null,"abstract":"Pulp liquids by-product of fermentation of cocoa beans potential as raw material cocoa vinegar. However, there is still need for further fermentation to optimize the formation of acetic acid. Here we describe the production of vinegar from cocoa pulp liquids. We have tested various fermentation method and to define the best method to produce distillated cocoa vinegar. This research was conducted by treatment with various fermentation methods comprising 1-phase, 2-phase, and natural. Acetic acid (1-phase) fermentation using Acetobacter aceti inoculum, with variations in alcohol additions of 6, 8, 10, and 12%. Alcohol and acetic acid (2-phase) fermentation using Saccharomyces cerevisiae and Acetobacter aceti inoculum, with variations in sugar additions of 4, 6, 8 and 10%. And other fermentation methods are natural aerobic fermentation (without inoculum and carbon source). All further fermentation methods were carried out at room temperature for 25 days and were categorized into four groups to obtain 36 experimental units. The results showed that the further fermentation methods and the addition of sugar or alcohol affected the characteristics of distillate of cocoa vinegar, such as organic acids, total soluble solids (TSS), sugar, salt, alcohol, and acidity (pH). It concludes that the single phase fermentation and addition of 10% alcohol is producing higher content of acetic and propionic acid of cocoa vinegar distillate as well as higher TSS and sugar, but lower salt contents and acidity (pH).","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90696438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of probiotics in lowering severity of symptoms in urban women with functional constipation: A randomized double-blind controlled trial","authors":"M. Abdullah, H. Maulahela, A. P. Utari, P. Kusumo, A. Soebandrio, I. Surono, D. Makmun","doi":"10.1063/1.5125530","DOIUrl":"https://doi.org/10.1063/1.5125530","url":null,"abstract":"Functional constipation is one of the most common gastrointestinal disorders worldwide, especially in women. Probiotic consumption is commonly used to alleviate symptoms of constipation but the mechanism and the result remain unclear. Hence, this study was conducted to assess the effect of probiotics on constipation symptoms using PAC-SYM© (Patient Assessment of Constipation Symptoms) questionnaire. This was a randomized double-blind controlled trial (RCT) study, consisting of 73 subjects of urban women with functional constipation. This study compared three weeks of probiotic (Lactobacillus plantarum IS 10506) administration with placebo and evaluated improvements in PAC-SYM© scores before and after intervention. The subjects were divided into two groups, probiotics group (n = 34) and placebo group (n = 39), then were compared for their mean difference of PAC-SYM© scores. Our study suggested improvements of symptoms after three weeks of probiotic administration, as shown by the total score of PAC-SYM© (mean difference 7.65, 95% CI 3.1-12.20; p=0.001) along with three domains of PAC-SYM©, abdominal symptoms (mean difference 3.0, 95% CI 1.3-4.7; p=0.001), rectal symptoms (mean difference 1.62, 95% CI 0.12-3.13; p=0.034) and stool symptoms (mean difference 3.02, 95% CI 0.58-5.45; p=0.016. Probiotics was superior than placebo in improvements of constipation symptoms in urban women with functional constipation.Functional constipation is one of the most common gastrointestinal disorders worldwide, especially in women. Probiotic consumption is commonly used to alleviate symptoms of constipation but the mechanism and the result remain unclear. Hence, this study was conducted to assess the effect of probiotics on constipation symptoms using PAC-SYM© (Patient Assessment of Constipation Symptoms) questionnaire. This was a randomized double-blind controlled trial (RCT) study, consisting of 73 subjects of urban women with functional constipation. This study compared three weeks of probiotic (Lactobacillus plantarum IS 10506) administration with placebo and evaluated improvements in PAC-SYM© scores before and after intervention. The subjects were divided into two groups, probiotics group (n = 34) and placebo group (n = 39), then were compared for their mean difference of PAC-SYM© scores. Our study suggested improvements of symptoms after three weeks of probiotic administration, as shown by the total score of PAC-SYM© (m...","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86510362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of moisture content in rice using non-destructive short-wave near infrared spectroscopy","authors":"M. Makky, Santosa, R. Putri, K. Nakano","doi":"10.1063/1.5125518","DOIUrl":"https://doi.org/10.1063/1.5125518","url":null,"abstract":"Determination of indices of the indigenous west Sumatran rice varieties was done to rapidly evaluate its moisture contents (MC) by means of non-destructive evaluation. The objective of this study was to identify the MC of two indigenous rice from west Sumatra, Indonesia, namely Junjuangan and Mundam, cultivars. The evaluation was rapidly performed by means of non-destructive evaluation using 1000-2500 nm short wave infrared (SWIR) spectral assessment. The paddy grains samples with identical MC were put into 10 cm petri dish and measured using SWIR spectrophotometer. The grains’ actual MC was then measured by primary method, based on weight measurement. In this study, the spectral data of the grains was then processed by means of Principal Component Analysis (PCA) before correlated with its MCs by Partial Least Square (PLS) method. The model calibration obtained for SWIR spectrophotometer showed correlation of 0.826 and 0.955, with root mean squared error calibration (RMSEC) of 2.97 and 1.4 for Junjuangan and Mundam rice respectively. Moreover, model validation produced correlation of 0.788 and 0.968, RMSEP of 3.8 and 1.29, and bias of 0.193 and 0.171 for Junjuangan and Mundam rice, respectively. The results indicated that the MC of paddy grains could be precisely identified by means of non-destructive evaluation using spectral analysis.Determination of indices of the indigenous west Sumatran rice varieties was done to rapidly evaluate its moisture contents (MC) by means of non-destructive evaluation. The objective of this study was to identify the MC of two indigenous rice from west Sumatra, Indonesia, namely Junjuangan and Mundam, cultivars. The evaluation was rapidly performed by means of non-destructive evaluation using 1000-2500 nm short wave infrared (SWIR) spectral assessment. The paddy grains samples with identical MC were put into 10 cm petri dish and measured using SWIR spectrophotometer. The grains’ actual MC was then measured by primary method, based on weight measurement. In this study, the spectral data of the grains was then processed by means of Principal Component Analysis (PCA) before correlated with its MCs by Partial Least Square (PLS) method. The model calibration obtained for SWIR spectrophotometer showed correlation of 0.826 and 0.955, with root mean squared error calibration (RMSEC) of 2.97 and 1.4 for Junjuangan ...","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89489939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening, identification and optimization of extracellular lipase production of yeast (Cryptococcus flavescens) isolated from a tree canopy fern in the Mount Makiling Forest Reserve, Philippines","authors":"F. Elegado, Charisse Leanne B. Legaspi, Joseph Martin Paet, Florabelle Querubin, Jarel Elgin Tolentino, J. Vilela, A. Paguio, J. Maloles, J. Zarate","doi":"10.1063/1.5125533","DOIUrl":"https://doi.org/10.1063/1.5125533","url":null,"abstract":"Lipases (triacylglycerol acylhydrolases, E.C. 3.1.1.3) are enzymes vastly used in industrial applications. The current study aims to screen lipase-producing yeasts isolated from a tree canopy fern from the Makiling Forest Reserve (MFR), Philippines and to optimize conditions that can maximize the mass production and activity of the enzyme. From the 144 isolates, B1-7 showed the highest lipase activity in both solid (EIA 7.6) and liquid selection media (0.082 U/mL-min). Molecular identification using Internally Transcribed Spacer (ITS) primers and microscopic observation revealed that the isolate was Cryptococcus flavescens, a generally regarded as safe (GRAS) microorganism. Response Surface Method (Box-Behnken Design) showed that the maximum lipase activity (0.66 U/mL-min) and a biomass of 4 g/L were achieved at 5.0 Carbon:Nitrogen ratio, pH 6.0 and 0.5% inducer (Tween 20). Also, C:N-% inducer interaction and inducer concentration significantly affected lipase activity. After a 72h fed-batch fermentation experiment, lipase activity was ten-fold lower than the optimization results and a negative correlation (r=-0.405) between lipase activity and biomass suggested the non-dependence of lipase activity to biomass availability. Lastly, total sugar concentration remained constant implying that the organism used the degradative products of lipase as its carbon source. In conclusion, C. flavescens from MFR can be utilized for mass lipase production, but it was recommended that other parameters be examined and optimized.Lipases (triacylglycerol acylhydrolases, E.C. 3.1.1.3) are enzymes vastly used in industrial applications. The current study aims to screen lipase-producing yeasts isolated from a tree canopy fern from the Makiling Forest Reserve (MFR), Philippines and to optimize conditions that can maximize the mass production and activity of the enzyme. From the 144 isolates, B1-7 showed the highest lipase activity in both solid (EIA 7.6) and liquid selection media (0.082 U/mL-min). Molecular identification using Internally Transcribed Spacer (ITS) primers and microscopic observation revealed that the isolate was Cryptococcus flavescens, a generally regarded as safe (GRAS) microorganism. Response Surface Method (Box-Behnken Design) showed that the maximum lipase activity (0.66 U/mL-min) and a biomass of 4 g/L were achieved at 5.0 Carbon:Nitrogen ratio, pH 6.0 and 0.5% inducer (Tween 20). Also, C:N-% inducer interaction and inducer concentration significantly affected lipase activity. After a 72h fed-batch fermentation ...","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90091655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}