PENELITIAN PERUBAHAN WARNA DAN ETILEN SELAMA PEMERAMAN DAUN TEMBAKAU KULTIVAR MADURA SERTA PENGARUHNYA TERHADAP MUTU TEMBAKAU RAJANGAN

J. Purdiyanto
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Abstract

Colour alternation and et/tylene changes during ripening tobacco leaves cultivar Madura and its effect to the quality of slice Madura tobaccoThe colour change and elhylene production in the leaves of Madura tobacco (Nicotiana tabacum) during ripening was studied at "Labora¬ torium Biokimia Pusat Antar Universitas Pangan dan Gizi Universitas Gadjahmada Yogyakata" and "Laboratorium Inti dan Atom Jurusan I'isika, FMIPA Universitas Gadjahmada Yogyakata". The change of colour was measured with Chrommameter CR-200. while the production of ethylcne, ACC (1-aminocyclopropanc-l-carboxylic acid) and MACC (malonyl-ACC) were measured with Photoaccuslic Spectroscopy, from the sample of 100 kg tobacco leaves cv. Jepon grown from April to September 1995 in Plakpak, Pcgantenan, Pamekasan, East Java. The experiment was designed as a completely randomized design in three replicates. The leaves samples were taken randomly from the mature middle leaves Results showed that endogenous ethylcne production al the irst day al a level of 169 ppb. was able to initiate ripening. Conversion percentage of ACC to ethylcne for 8 days ripening increased from 7.7 up lo 61.4 %. F tests least signiicantly different (1%). showed that yellow intensity signiicantly increased on the second day. This indicated thai ripening process was stated on the second day. On the other hand green intensity significantly decreased at the ifth day, thus no chlorophyl degradation. Brightness was signiicantly observed on Ihe third day of ripening, meaning that ripening stated on the third day. Based on the three criteria, the best ripening period was from the third lo the ilth day. This implies that the farmers will have three day periods of ripening which can be arranged to overcome the shotage of man power for slicing the leaves.
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研究库蒂瓦·马杜拉烟草叶的颜色和乙烯变化以及它对烟草质量的影响
在“anantar Universitas Pangan dan Gizi Universitas Gadjahmada Yogyakata”和“Laboratorium Inti dan Atom Jurusan I’isika, FMIPA Universitas Gadjahmada Yogyakata”研究了Madura烟草(Nicotiana tabacum)成熟过程中叶片颜色变化和乙烯的产生。用CR-200色度仪测定颜色变化。利用光电光谱法测定了100 kg烟叶样品中乙炔、1-氨基环丙基-l-羧酸(ACC)和丙二醇-ACC的产量。1995年4月至9月在东爪哇的Plakpak, Pcgantenan, Pamekasan种植Jepon。试验采用完全随机设计,分3个重复。结果表明,第一天的内源乙烯产量为169 ppb。能够开始成熟。8 d催熟过程中ACC对乙炔的转化率由7.7%上升至61.4%。F检验差异最小(1%)。结果表明,第2天黄光强度显著增强。这表明泰国的成熟过程是在第二天陈述的。另一方面,第5天绿光强度显著降低,没有叶绿素降解。在成熟的第三天,亮度被显著观察到,这意味着在第三天成熟。根据三个指标,最佳成熟期为第3 ~第9天。这意味着农民将有三天的成熟期,这可以安排以克服切叶子的人力短缺。
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