Determination of the optimal conditions for the extraction of anthraglycosides of alder buckthorn bark, development and validation of a method for their quantitative determination by high performance liquid chromatography

Abstract

Objective: optimization of extraction conditions for anthraglycosides of alder buckthorn bark, development and validation of a method for their quantitative determination using high performance liquid chromatography. Materials and methods. The object of the study was alder buckthorn bark from different manufacturers. The work was performed on an Agilent 1100 liquid chromatograph complete with a G1311A four-solvent supply and degassing system, a G1315B diode array detector, a G1316A column thermostat, and a G1313A automatic sample injection device. Chromatographic analysis was carried out on a Zorbax SB-C18 reverse-phase column (4.6×250 mm, 5 μm) in a gradient elution mode of the mobile phase consisting of acetonitrile and highly purified water, adjusted to pH 2 with phosphoric acid. Results. As a result of the study, the optimal conditions for the chromatographic separation of anthraglycosides of alder buckthorn bark were determined. The optimal conditions for their extraction have been established: the grinding of plant raw materials is 250 µm, the extractant is ethyl alcohol 40%, the extraction temperature is 80℃, the extraction time is 90 minutes, the ratio of raw materials and extractant is 1:50. It has been proved that the developed method for the quantitative determination of anthraglycos