Phytochemical properties, antibacterial and anti-free radical activities of the phenolic extracts of Retama raetam (Forssk) Webb. & Berthel. collected from Algeria Desert

IF 1 Q4 CHEMISTRY, MULTIDISCIPLINARY Ovidius University Annals of Chemistry Pub Date : 2021-01-01 DOI:10.2478/auoc-2021-0005
A. Chouikh, F. Alia
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引用次数: 3

Abstract

Abstract This study aimed to evaluate the phytochemical properties, antibacterial and anti free-radical activities of Retama raetam extracts which is growing in the South-East of the Algeria Desert. The chemical screening showed the presence of many secondary metabolites such as tannins catechin, sterols and terpenes, and the absence of gallic tannins compounds. The obtained results demonstrated that the methanolic extract has shown moderate total phenolic and flavonoids contents (31.59 ± 2.82 mg AG E/g extract and 14.35 ±1.02 mg Qu E/g extract respectively). In the free radical DPPH test, the values of IC50 were converging in all extracts of R. raetam. The antibacterial activity of extracts has been tested against ten bacterial strains, were registered as the best inhibition zones with Vibrio cholera, Micrococcus luteus and Serratia marcescen. Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 25923 strains showed high resistance against most of all concentrations of extracts, and we noted the Gram-negative bacteria strains are the most sensitive to the different extracts of the plant. The qualitative analysis of extracts by using HPLC showed the contrast in presence of the phenolic compounds, such as in ethyl acetate extract registered absence of chlorogenic acid, also the absence of caffeic acid in 1-butanol extract. These results confirmed of the phenolic extracts of this plant are a source natural alternative to antibiotics and antioxidants.
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苦参酚类提取物的植物化学性质、抗菌和抗自由基活性。& Berthel。采自阿尔及利亚沙漠
摘要本研究旨在评价生长在阿尔及利亚沙漠东南部的雷塔马提取物的植物化学性质、抗菌和抗自由基活性。化学筛选显示存在许多次级代谢物,如单宁、儿茶素、甾醇和萜烯,没有没食子单宁化合物。结果表明,甲醇提取物的总酚和总黄酮含量适中(分别为31.59±2.82 mg AG E/g和14.35±1.02 mg Qu E/g)。在自由基DPPH试验中,各提取物的IC50值趋于一致。对10种细菌进行了抑菌试验,对霍乱弧菌、黄体微球菌和粘质沙雷氏菌均有较好的抑菌效果。铜绿假单胞菌ATCC 27853和金黄色葡萄球菌ATCC 25923菌株对大多数浓度的提取物均表现出较高的抗性,革兰氏阴性菌菌株对不同浓度的提取物最敏感。采用高效液相色谱法对提取物进行定性分析,发现乙酸乙酯提取物中不含绿原酸,1-丁醇提取物中不含咖啡酸。这些结果证实了这种植物的酚类提取物是抗生素和抗氧化剂的天然替代品。
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Ovidius University Annals of Chemistry
Ovidius University Annals of Chemistry CHEMISTRY, MULTIDISCIPLINARY-
自引率
11.10%
发文量
20
审稿时长
5 weeks
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