[PURIFICATION OF TOXOHORMONE].

Abstract

In 1948, Nakahara and Fukuoka15) first isolated toxohormone from a tumor as a factor depressing liver catalase. Subsequently, it was reported that injection of the toxohormone fraction caused thymus involution,6) anemia,6,7,11) depression of liver tryptophan pyrrolase,10) DPN synthetase,18,20) and the plasma iron level14) as well as reduction of liver catalase activity. Fujii et al.4) obtained a basic protein fraction with toxohormone activities from Rhodamine sarcoma. They subjected it to carboxymethylcellulose column chromatography and separated a plasma iron-depressing factor from factors depressing liver catalase and tryptophan pyrrolase. Accordingly, Fujii et al.5) proposed the "toxohormone complex theory." The present paper reports further purification of the plasma iron-depressing factor and analysis of its chemical composition.