Biogeochemical activity of different phinotypes of Trifolium repens L. to some heavy metals

Abstract

Aim. Investigate the general ability of different phenotypes of Trifolium repens L. according to the pattern of white marking on a leaf to the concentration of mobile forms of iron (Fe), manganese (Mn), copper (Cu) and zinc (Zn). Methods. Determination of Fe, Mn, Cu and Zn content in Trifolium repens L. in soil and leaves was performed by atomic absorption method using ICP-AES analyzer. Collection of leaves and selection of soil samples for research was carried out in 2021 during the period of mass flowering of Trifolium repens L. from randomized areas of pasture. Identification of drawings of white marking on a leaf  was carried out according to the method of I. T. Paponova and P. Ya. Schwartzman, using the tables of J. L. Briubecker. To characterize the biogenic migration of Fe, Mn, Cu and Zn and biogeochemical features of different phenotypes of Trifolium repens L. used methods developed by I. A Avessalomova. Results. In the sample of leaves of Trifolium repens L. from pasture 5 phenotypes (O, A, AH, BH, С) were found, the genotypes of which are formed by a series of 6 alleles of the V gene. The most common was the phenotype BH, the smallest was the share of the phenotype AH. The highest total ability to concentrate the mobile forms of Fe, Mn, Cu and Zn was found by phenotype BH, the lowest – phenotype A. Conclusions. Phenotypes O, A, AH, BH, С differ in biogeochemical activity in terms of the concentration of mobile forms of Fe, Mn, Cu and Zn. According to the general ability to accumulate these elements, these phenotypes form the following descending order: BH >O >С >AH >A.