Aim. The aim of the research was to investigate the induction of the Triticum aestivum L. non-specific tolerance by ferulic acid as a biotic elicitor and donor of nitric oxide as a signal molecule to hypoxia and wounding stress. Methods. Winter wheat plants cv. Kesariia Polis`ka were grown and had treated by ferulic acid and sodium nitroprusside (SNP) solutions then wounding or flooding stress started. The hydrogen peroxide content was measured in leaves during the experimental period. Results. It is shown that an influence of the nitric oxide donor balanced the hydrogenous peroxide level in wounded leaves close to control. After two days of flooding stress plants treated by ferulic acid decreased hydrogen peroxide level on 15 % against threefold decreasing in non-treated stressed plants. Donor NO treatment also paid contribution to hydrogen peroxide equilibrium during the first hour under hypoxia conditions caused by flooding stress and decreased hydrogen peroxide content till the third day of stress. Conclusions. Ferulic acid and nitric oxide donor are perspective components for complex defense against hypoxia and wounding in wheat plants.
{"title":"Effect of biotic elicitor and donor NO treatment in complex defence of wheat plants against hypoxia and wounding stress","authors":"I. Zhuk, J. V. Shylina, A. P. Dmytriev","doi":"10.7124/feeo.v30.1464","DOIUrl":"https://doi.org/10.7124/feeo.v30.1464","url":null,"abstract":"Aim. The aim of the research was to investigate the induction of the Triticum aestivum L. non-specific tolerance by ferulic acid as a biotic elicitor and donor of nitric oxide as a signal molecule to hypoxia and wounding stress. Methods. Winter wheat plants cv. Kesariia Polis`ka were grown and had treated by ferulic acid and sodium nitroprusside (SNP) solutions then wounding or flooding stress started. The hydrogen peroxide content was measured in leaves during the experimental period. Results. It is shown that an influence of the nitric oxide donor balanced the hydrogenous peroxide level in wounded leaves close to control. After two days of flooding stress plants treated by ferulic acid decreased hydrogen peroxide level on 15 % against threefold decreasing in non-treated stressed plants. Donor NO treatment also paid contribution to hydrogen peroxide equilibrium during the first hour under hypoxia conditions caused by flooding stress and decreased hydrogen peroxide content till the third day of stress. Conclusions. Ferulic acid and nitric oxide donor are perspective components for complex defense against hypoxia and wounding in wheat plants.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75577911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. Determine the type of quantitative traits inheritance in einkorn wheat F1 hybrids. Methods. Hybridological and morphometric analyses. Results. In the combination ♀T. urartu × ♂T. sinskajae, the hybrid caryopses did not set; in the reciprocal combination, the set rate was 28 %. In combinations involving T. boeoticum, T. monococcum, T. sinskajae the setting ranged from 37 % to 54 %. In different combinations, hybrids showed overdominance, hybrid depression, dominance of parental traits with a higher and lower trait value, rarely intermediate inheritance. The combinations in which T. sinskajae is involved are of interest due to heterosis in grain weight of per ear, spikelet number in ear, grain number in ear and less hullness. At the same time, the dominance degree for this trait correlates with the indicators of the dominance degree in length, width and area of flag leaf (r: 0.62–0.71). Conclusions. Einkorn hybrids with the participation of naked wheat T. sinskajae are promising in terms of spikelet number per ear, grain number per ear, ear weight, grain weight per ear, reduced hullness.
{"title":"Inheritance of quantitative traits in F1 hybrids of einkorn wheat","authors":"H. Fu, R. Bohuslavskyi, L. Atramentova","doi":"10.7124/feeo.v31.1501","DOIUrl":"https://doi.org/10.7124/feeo.v31.1501","url":null,"abstract":"Aim. Determine the type of quantitative traits inheritance in einkorn wheat F1 hybrids. Methods. Hybridological and morphometric analyses. Results. In the combination ♀T. urartu × ♂T. sinskajae, the hybrid caryopses did not set; in the reciprocal combination, the set rate was 28 %. In combinations involving T. boeoticum, T. monococcum, T. sinskajae the setting ranged from 37 % to 54 %. In different combinations, hybrids showed overdominance, hybrid depression, dominance of parental traits with a higher and lower trait value, rarely intermediate inheritance. The combinations in which T. sinskajae is involved are of interest due to heterosis in grain weight of per ear, spikelet number in ear, grain number in ear and less hullness. At the same time, the dominance degree for this trait correlates with the indicators of the dominance degree in length, width and area of flag leaf (r: 0.62–0.71). Conclusions. Einkorn hybrids with the participation of naked wheat T. sinskajae are promising in terms of spikelet number per ear, grain number per ear, ear weight, grain weight per ear, reduced hullness.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74822749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. Assessment of the level of productivity, heterosis and the nature of inheritance in hybrids (F3 and F5) of alfalfa under conditions of high soil acidity (pH 5.2-5.3). Methods. Field, laboratory. Results. The results of research (2013-2020) on the level of productivity and effects of heterosis, the nature of inheritance of traits of fodder and seed productivity in 37 hybrid populations (F3 and F5), created with samples of alfalfa sowing and variable of different ecological and geographical origin, naturally soil background with high acidity (pH 5.2-5.3). Conclusions. Hybrid pop-ulations of alfalfa with a combination of high fodder and seed productivity against the background of soil acidity have been selected and proposed for use in the selection process: Regina / Zhidrune, Sinyukha / Mega, Sinyukha / Yaroslav-na, Grilys / Mega, Grilys / Regina. Samples Mega / Regina, Vika / Regina, Zhidrune / Regina, Zhidrune / Sinyukha, Mega / Grilys, Grilys / Vika Yaroslavna / Vika, Yaroslavna / Zhidrune, Grilys / Zhidrune, Vika can be used separately in selection process after further researches on forage productivity and seed – Regina / Mega.
{"title":"Manifestation of heterosis of signs of productivity in hybrids (F3 and F5) of lucern of seeding under conditions of high soil acidity","authors":"V. Buhayov, V. Horenskyy, V. S. Mamalyga","doi":"10.7124/feeo.v31.1481","DOIUrl":"https://doi.org/10.7124/feeo.v31.1481","url":null,"abstract":"Aim. Assessment of the level of productivity, heterosis and the nature of inheritance in hybrids (F3 and F5) of alfalfa under conditions of high soil acidity (pH 5.2-5.3). Methods. Field, laboratory. Results. The results of research (2013-2020) on the level of productivity and effects of heterosis, the nature of inheritance of traits of fodder and seed productivity in 37 hybrid populations (F3 and F5), created with samples of alfalfa sowing and variable of different ecological and geographical origin, naturally soil background with high acidity (pH 5.2-5.3). Conclusions. Hybrid pop-ulations of alfalfa with a combination of high fodder and seed productivity against the background of soil acidity have been selected and proposed for use in the selection process: Regina / Zhidrune, Sinyukha / Mega, Sinyukha / Yaroslav-na, Grilys / Mega, Grilys / Regina. Samples Mega / Regina, Vika / Regina, Zhidrune / Regina, Zhidrune / Sinyukha, Mega / Grilys, Grilys / Vika Yaroslavna / Vika, Yaroslavna / Zhidrune, Grilys / Zhidrune, Vika can be used separately in selection process after further researches on forage productivity and seed – Regina / Mega.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84513213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. Obtaining of the tomato lines of cvs. Money Maker and Lahidny in the presence of polyfunctional preparations Avercom and Avercom Nova and cytological analysis of their influence on tomato root cells. Methods. Cotyledons of 10–11-day-old tomato seedlings were cultivated on MST medium for 1–3 months in the presence of 1 mg/l zeatin, 1 mg/l indole-acetic acid and 25 µl/l of Avercom or Avercom Nova. Then, regenerated lines were grown on MST medium supplemented with biopreparations for 1 month. The roots of tomato plants were fixed in the solution of ethanol and glacial acetic acid (3:1), stained in 1 % acetoorseine solution and investigated at x600 magnification. Results. In this work, the tomato lines were obtained and grown in the presence of biopreparations Avercom or Avercom Nova. It was shown that mitotic index (MI) in the root meristematic cells of the plants grown in the presence of biopreparations was at 0.76–1.23 %, and it was higher than in the control plants (0.65–0.9 %). Moreover, the thickened cell walls of root cells of plants grown in the presence of biopreparations was detected compared to control plants. Conclusions. Tomato plants grown in the presence of biopreparations Avercom or Avercom Nova are characterized not only by the increased MI of meristematic cells but also by the thickened cell walls of root cells which indicate the growth stimulating effects of biopreparations and their capacity to enhance the resistance of plants to stressful factors.
{"title":"Growth stimulating activity of avermectin-containing preparations on tomato lines in vitro","authors":"A. Buziashvili, H. S. Brylenko, A. Yemets","doi":"10.7124/feeo.v30.1460","DOIUrl":"https://doi.org/10.7124/feeo.v30.1460","url":null,"abstract":"Aim. Obtaining of the tomato lines of cvs. Money Maker and Lahidny in the presence of polyfunctional preparations Avercom and Avercom Nova and cytological analysis of their influence on tomato root cells. Methods. Cotyledons of 10–11-day-old tomato seedlings were cultivated on MST medium for 1–3 months in the presence of 1 mg/l zeatin, 1 mg/l indole-acetic acid and 25 µl/l of Avercom or Avercom Nova. Then, regenerated lines were grown on MST medium supplemented with biopreparations for 1 month. The roots of tomato plants were fixed in the solution of ethanol and glacial acetic acid (3:1), stained in 1 % acetoorseine solution and investigated at x600 magnification. Results. In this work, the tomato lines were obtained and grown in the presence of biopreparations Avercom or Avercom Nova. It was shown that mitotic index (MI) in the root meristematic cells of the plants grown in the presence of biopreparations was at 0.76–1.23 %, and it was higher than in the control plants (0.65–0.9 %). Moreover, the thickened cell walls of root cells of plants grown in the presence of biopreparations was detected compared to control plants. Conclusions. Tomato plants grown in the presence of biopreparations Avercom or Avercom Nova are characterized not only by the increased MI of meristematic cells but also by the thickened cell walls of root cells which indicate the growth stimulating effects of biopreparations and their capacity to enhance the resistance of plants to stressful factors.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76085017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Determination of the breeding value of the presence or absence of ear spines and anthocyanin stem color in bread winter wheat plants in the steppes of the Black Sea coast of Ukraine. Methods. Growing plants in the field. Phenological observations, weight of 1000 grains, analysis of variance and statistical significantly of the difference by t - criterion in pairwise comparison. Results. Groups of lines with the presence or absence of ear spines and with anthocyanin or green color, as well as with different combinations of both phenotypic traits did not differ significantly in economically valuable traits, with one exception. Plants with anthocyanin-colored stems were, on average, statistical significantly five centimeters taller than those with green color. Among the lines that exceeded the standard varieties Antonivka and Kuialnyk, in terms of yield, there are lines of all possible combinations of stem color and the presence or absence of spines. Conclusions. The phenotypic differences of recombinant lines in stem color (anthocyanin or green) or the presence or absence of spines, as well as their various combinations, are not significantly related to differences in genotypes before earing period, plant height, 1000 grain weight and grain yield.
{"title":"Relationship between the stem color and the ear spines presense with economically valuable traits of bread winter wheat","authors":"V. Fedorova, V. Fait, H. A. Zelenina","doi":"10.7124/feeo.v31.1493","DOIUrl":"https://doi.org/10.7124/feeo.v31.1493","url":null,"abstract":"Determination of the breeding value of the presence or absence of ear spines and anthocyanin stem color in bread winter wheat plants in the steppes of the Black Sea coast of Ukraine. Methods. Growing plants in the field. Phenological observations, weight of 1000 grains, analysis of variance and statistical significantly of the difference by t - criterion in pairwise comparison. Results. Groups of lines with the presence or absence of ear spines and with anthocyanin or green color, as well as with different combinations of both phenotypic traits did not differ significantly in economically valuable traits, with one exception. Plants with anthocyanin-colored stems were, on average, statistical significantly five centimeters taller than those with green color. Among the lines that exceeded the standard varieties Antonivka and Kuialnyk, in terms of yield, there are lines of all possible combinations of stem color and the presence or absence of spines. Conclusions. The phenotypic differences of recombinant lines in stem color (anthocyanin or green) or the presence or absence of spines, as well as their various combinations, are not significantly related to differences in genotypes before earing period, plant height, 1000 grain weight and grain yield.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90659082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim: The aim of the study was to establish the interspecific features of the functioning of the nucleolus-forming organizer on the example of 6 species of the family Pinaceae. Methods. The study involved samples of different species of conifers that grow within the natural range and conditions of introduction. Silver nitric acid staining was used to visualize the nucleoli. Quantitative and dimensional features of the nucleus and nucleoli in the interphase cells of seedlings were studied, and the values of the nucleus-nucleolus ratio were calculated. Results. Interspecific polymorphism in the number and size of nucleoli, nuclei and nucleus-nucleolus ratio in six species and forms of the Pinaceae L. family was revealed. Conclusions. The studied species of conifers obviously have interspecific differentiation by nucleus-nucleolus parameters. The studied characteristics are influenced not only by the species, age and condition of the plant, but also by the natural and climatic conditions in which the population exists.
{"title":"Nucleus-nucleolus features of certain species of the Pinaceae family","authors":"Yu. O. Bilonozhko, I. Korshikov","doi":"10.7124/feeo.v30.1458","DOIUrl":"https://doi.org/10.7124/feeo.v30.1458","url":null,"abstract":"Aim: The aim of the study was to establish the interspecific features of the functioning of the nucleolus-forming organizer on the example of 6 species of the family Pinaceae. Methods. The study involved samples of different species of conifers that grow within the natural range and conditions of introduction. Silver nitric acid staining was used to visualize the nucleoli. Quantitative and dimensional features of the nucleus and nucleoli in the interphase cells of seedlings were studied, and the values of the nucleus-nucleolus ratio were calculated. Results. Interspecific polymorphism in the number and size of nucleoli, nuclei and nucleus-nucleolus ratio in six species and forms of the Pinaceae L. family was revealed. Conclusions. The studied species of conifers obviously have interspecific differentiation by nucleus-nucleolus parameters. The studied characteristics are influenced not only by the species, age and condition of the plant, but also by the natural and climatic conditions in which the population exists.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84928528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. The aim of the work is to establish the level of similarity of the primary structure and organization of landomycin clusters of streptomycetes. Methods. Information on nucleotide sequences and annotations of streptomycetic clusters of landomycin biosynthesis genes AF080235,1, KJ645792.1, HQ828984.1 and genomes of strains S. cyanogenus S136, S. globisporus 1912-4Crt is freely available in GenBank. Analysis of the nucleotide sequences of streptomycetes was performed using the program BLASTN from the server NSBI. Results. The remote localization of a number of probable lan-cluster genes (prx, lanW, lanYR) has been established. The sequences of similar lndU / lanU genes and lndY / lanY genes, which are not necessary for landomycin biosynthesis, have been shown to be the least similar. It is shown that the lan-cluster of S. cyanogenus S136 is expected to be localized outside the cortical region of the chromosome of the strain. Landomycin-producing streptomycetes are isolated from soil samples from different ecological niches on different continents of the planet. Conclusions. The difference in the organization of lan- and lnd-clusters is expressed in the remote localization of a number of genes (prx, lanW, lanYR).
{"title":"Similarity and difference of organizations of streptomycetic landomycins clusters","authors":"L. Polishchuk, V. Lukyanchuk","doi":"10.7124/feeo.v31.1479","DOIUrl":"https://doi.org/10.7124/feeo.v31.1479","url":null,"abstract":"Aim. The aim of the work is to establish the level of similarity of the primary structure and organization of landomycin clusters of streptomycetes. Methods. Information on nucleotide sequences and annotations of streptomycetic clusters of landomycin biosynthesis genes AF080235,1, KJ645792.1, HQ828984.1 and genomes of strains S. cyanogenus S136, S. globisporus 1912-4Crt is freely available in GenBank. Analysis of the nucleotide sequences of streptomycetes was performed using the program BLASTN from the server NSBI. Results. The remote localization of a number of probable lan-cluster genes (prx, lanW, lanYR) has been established. The sequences of similar lndU / lanU genes and lndY / lanY genes, which are not necessary for landomycin biosynthesis, have been shown to be the least similar. It is shown that the lan-cluster of S. cyanogenus S136 is expected to be localized outside the cortical region of the chromosome of the strain. Landomycin-producing streptomycetes are isolated from soil samples from different ecological niches on different continents of the planet. Conclusions. The difference in the organization of lan- and lnd-clusters is expressed in the remote localization of a number of genes (prx, lanW, lanYR).","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83410419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. Using the method of colorimetry, to investigate the features of the manifestation of pink color of the petals of the corolla in cultivated and wild species of flax. Methods. Colorimetric, descriptive, statistical. Results. Different colors of petals of a number of flax lines with the use of instrumental technologies are analyzed and their numerical characteristics in the channels of red, green and blue colors of the spectrum are established. The obtained numerical characteristics of color are characterized by rather small variation that allows to use this technology for reliable identification of the samples of flax differing in color of petals or other parts of a plant. Conclusions. It was found that in all genotypes of flax with pink color of the petals in numerical terms, the red color component has similar values and predominates over other color components. L. grandiflorum was found to have the highest percentage of color components, which differs significantly from other genotypes studied. A large proportion of red color was also found in the variety Rumyanets and L. pubescens. And in the pink-flowered lines of cultivated flax M-66 and LR-1-1 a uniform ratio of color components was found.
{"title":"Application of colorimetry method for establishment of features of pink and red color of flax petals","authors":"G. Sklyarenko, I. O. Poliakova, V. M. Nikonova","doi":"10.7124/feeo.v31.1489","DOIUrl":"https://doi.org/10.7124/feeo.v31.1489","url":null,"abstract":"Aim. Using the method of colorimetry, to investigate the features of the manifestation of pink color of the petals of the corolla in cultivated and wild species of flax. Methods. Colorimetric, descriptive, statistical. Results. Different colors of petals of a number of flax lines with the use of instrumental technologies are analyzed and their numerical characteristics in the channels of red, green and blue colors of the spectrum are established. The obtained numerical characteristics of color are characterized by rather small variation that allows to use this technology for reliable identification of the samples of flax differing in color of petals or other parts of a plant. Conclusions. It was found that in all genotypes of flax with pink color of the petals in numerical terms, the red color component has similar values and predominates over other color components. L. grandiflorum was found to have the highest percentage of color components, which differs significantly from other genotypes studied. A large proportion of red color was also found in the variety Rumyanets and L. pubescens. And in the pink-flowered lines of cultivated flax M-66 and LR-1-1 a uniform ratio of color components was found.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90236065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. The effect of chronic ultraviolet B (UV-B) radiation on shoots growth, content of photosynthetic pigments and hydrogen peroxide (HP) in the leaves of pea plants (Pisum sativum L.) was studied. Methods. Pea plants cultivar Gotivsky were irradiated by chronic UV-B during 5 days in the doses from 2.5 to 4 kJ/m2 per day with a power of 1 W/m2. The length of plant shoots was measured during 7 days after the end of radiation. Content of photosynthetic pigments and HP in leaves were measured on the 7 day after radiation. Results. It was shown that after the chronic UV-B radiation of pea plants with doses from 2.5 to 4 kJ/m2 per day, the length of shoots increased, most significantly after the dose of 3 kJ/m2 per day. Content of HP in mature leaves was in 2 times higher than the level of control in all variants, the content of chlorophyll and carotenoids decreased compared to the control, most significantly after a dose 4 kJ/m2 per day. Conclusions. It was shown that after the UV-B chronic radiation with doses from 2.5 to 4 kJ/m2 per day of pea plants, shoot growth increased. The content of HP in mature leaves increased, the content of photosynthetic pigments decreased. The chronic UV-B radiation caused the destruction of the pigment complex of mature pea leaves and oxidative stress, but the absence of UV-B in the light can cause the decrease of growth.
{"title":"Effect of chronic ultraviolet b radiation on pea plants","authors":"V. Zhuk, A. N. Mikheev, L. G. Ovsyannikova","doi":"10.7124/feeo.v30.1463","DOIUrl":"https://doi.org/10.7124/feeo.v30.1463","url":null,"abstract":"Aim. The effect of chronic ultraviolet B (UV-B) radiation on shoots growth, content of photosynthetic pigments and hydrogen peroxide (HP) in the leaves of pea plants (Pisum sativum L.) was studied. Methods. Pea plants cultivar Gotivsky were irradiated by chronic UV-B during 5 days in the doses from 2.5 to 4 kJ/m2 per day with a power of 1 W/m2. The length of plant shoots was measured during 7 days after the end of radiation. Content of photosynthetic pigments and HP in leaves were measured on the 7 day after radiation. Results. It was shown that after the chronic UV-B radiation of pea plants with doses from 2.5 to 4 kJ/m2 per day, the length of shoots increased, most significantly after the dose of 3 kJ/m2 per day. Content of HP in mature leaves was in 2 times higher than the level of control in all variants, the content of chlorophyll and carotenoids decreased compared to the control, most significantly after a dose 4 kJ/m2 per day. Conclusions. It was shown that after the UV-B chronic radiation with doses from 2.5 to 4 kJ/m2 per day of pea plants, shoot growth increased. The content of HP in mature leaves increased, the content of photosynthetic pigments decreased. The chronic UV-B radiation caused the destruction of the pigment complex of mature pea leaves and oxidative stress, but the absence of UV-B in the light can cause the decrease of growth.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89950076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. In current work we have analyzed 2664 genomes of Streptomyces spp. (publically available from GenBank) searching for complestatin-like biosynthetic gene clusters (BGCs). Then we aimed to reconstruct in silico putative biosynthetic pathways, encoded within these BGCs, estimating the chemical variability of complestatin-like compounds from natural sources. Methods. Widely accepted genome-mining tools and approaches for phylogenetic reconstruction were utilized in this work. Results. 53 genomes of Streptomyces spp. were found to contain complestatin-like BGCs, although only 33 BGCs were found within one contig – others were partial or highly fragmented. Reconstruction of multi-locus phylogeny for 33 found BGCs and complestatin BGC allowed to divide all these BGCs into five phylogenetic subgroups. Representatives of each subgroup exhibited characteristic organization of corresponding BGCs. Reconstruction of putative biosynthetic pathways allowed us to predict that discovered BGCs might potentially code the biosynthesis of new complestatin derivatives: norcomplestatin, N-malonyl-norcomplestatin, and N-acetyl-norcomplestatin. Conclusions. Complestatin-like BGCs are widely distributed among Streptomyces spp. and might encode novel complestain derivatives, which merits further experimental investigation.
{"title":"Distribution of biosynthetic gene clusters for complestatin and related compounds in Streptomyces spp.","authors":"O. Yushchuk, K. Zhukrovska, V. Fedorenko","doi":"10.7124/feeo.v30.1474","DOIUrl":"https://doi.org/10.7124/feeo.v30.1474","url":null,"abstract":"Aim. In current work we have analyzed 2664 genomes of Streptomyces spp. (publically available from GenBank) searching for complestatin-like biosynthetic gene clusters (BGCs). Then we aimed to reconstruct in silico putative biosynthetic pathways, encoded within these BGCs, estimating the chemical variability of complestatin-like compounds from natural sources. Methods. Widely accepted genome-mining tools and approaches for phylogenetic reconstruction were utilized in this work. Results. 53 genomes of Streptomyces spp. were found to contain complestatin-like BGCs, although only 33 BGCs were found within one contig – others were partial or highly fragmented. Reconstruction of multi-locus phylogeny for 33 found BGCs and complestatin BGC allowed to divide all these BGCs into five phylogenetic subgroups. Representatives of each subgroup exhibited characteristic organization of corresponding BGCs. Reconstruction of putative biosynthetic pathways allowed us to predict that discovered BGCs might potentially code the biosynthesis of new complestatin derivatives: norcomplestatin, N-malonyl-norcomplestatin, and N-acetyl-norcomplestatin. Conclusions. Complestatin-like BGCs are widely distributed among Streptomyces spp. and might encode novel complestain derivatives, which merits further experimental investigation.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79977602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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