T. Murata, Satoshi Amarume, T. Usui, H. Ohshima, K. Abukawa, Bunji Kanzaki, A. Hosono
{"title":"Measurement of lactose in bovine raw milk using oligosaccharide oxidase","authors":"T. Murata, Satoshi Amarume, T. Usui, H. Ohshima, K. Abukawa, Bunji Kanzaki, A. Hosono","doi":"10.1271/NOGEIKAGAKU1924.75.447","DOIUrl":null,"url":null,"abstract":"A novel method for measuring lactose in bovine raw milk has been developed. The method is based on measuring H2O2 produced from lactose by oligosaccharide oxidase through a coupled reaction involving peroxidase with a chromogenic reagent. The assay system gave a linear dose response of Lactose in the range of 25-300 μg/mL in 2-20 min of incubation. Lactose concentration in diluted bovine raw milk was directly determined with high precision by this method without any pretreatment. This is a quick and simple procedure and is suitable for multi-sample assay compared to the conventional Lane-Eynon method.","PeriodicalId":9443,"journal":{"name":"Bulletin of the Agricultural Chemical Society of Japan","volume":"35 1","pages":"447-449"},"PeriodicalIF":0.0000,"publicationDate":"2001-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of the Agricultural Chemical Society of Japan","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1271/NOGEIKAGAKU1924.75.447","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
A novel method for measuring lactose in bovine raw milk has been developed. The method is based on measuring H2O2 produced from lactose by oligosaccharide oxidase through a coupled reaction involving peroxidase with a chromogenic reagent. The assay system gave a linear dose response of Lactose in the range of 25-300 μg/mL in 2-20 min of incubation. Lactose concentration in diluted bovine raw milk was directly determined with high precision by this method without any pretreatment. This is a quick and simple procedure and is suitable for multi-sample assay compared to the conventional Lane-Eynon method.
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