A Comparison of Sedimentation Method and Active Sampler Analysis of Microbiological Indoor Air Quality - Case Study

W. Przystaś, E. Zabłocka-Godlewska, E. Melaniuk-Wolny
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Abstract

Abstract The indoor air quality is of great importance for the health of people. Appropriate selection of methods, parameters and conditions for the analysis makes it possible to obtain the results that reliably reflect the actual state of affairs. The aim of this study was to compare the results of microbiological air analysis in selected rooms of the Biotechnology Center obtained using the sedimentation method and the impaction method. During the research, the SMA (for total bacteria number) and Sabouraud media (for total fungi number) were exposed to different times in sedimentation analysis and to different air volumes in the impaction method. In the case of the sedimentation method, significant differences were found in the total number of bacteria and fungi in 3 out of 7 rooms depending on the time of exposure. In the case of the impaction method, it was 4 out of 7 rooms for bacteria and 2 out of 7 for fungi, depending on the analysed volume of air. The comparison of the methods showed that 4 out of 6 rooms had higher number of microorganisms when impactor was used.
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沉淀法与主动采样法对室内空气微生物质量的比较研究
摘要室内空气质量对人们的身体健康至关重要。适当选择分析的方法、参数和条件,可以获得可靠地反映实际情况的结果。本研究的目的是比较生物技术中心选定房间的微生物空气分析结果,采用沉降法和冲击法。在研究过程中,SMA(细菌总数)和Sabouraud培养基(真菌总数)在沉降分析中暴露于不同的时间,在冲击法中暴露于不同的空气体积。在沉淀法的情况下,根据暴露时间的不同,在7个房间中有3个房间的细菌和真菌总数存在显著差异。在撞击法的情况下,根据分析的空气体积,7个房间中有4个房间有细菌,7个房间中有2个房间有真菌。结果表明,采用冲击器时,6个房间中有4个房间的微生物数量较高。
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