Advanced Studies on Toxoplasma in Buffalo Meat

D. Hussein, A. Khashaba, Ayman M. Kamar
{"title":"Advanced Studies on Toxoplasma in Buffalo Meat","authors":"D. Hussein, A. Khashaba, Ayman M. Kamar","doi":"10.5455/ajvs.148101","DOIUrl":null,"url":null,"abstract":"Since buffalo meat, has been demonstrated to be a potential source of human infection, a careful evaluation of the prevalence of Toxoplasma infection in this meat is needed. Tissue cysts of Toxoplasma gondii are frequently found in the skeletal muscles of buffaloes. This study evaluated the prevalence of Toxoplasma gondii in the meat juice of buffalo meat samples via several diagnostic techniques to protect public health. Peptic digestion, histopathology, and serology were performed on meat juice from 100 buffalo meat samples from local butchers and retail beef markets. Eighteen samples (18%) were suspected of the presence of bradyzoites after digestion, and were subjected to histopathology which illustrated that only six samples (6%) were suspected to be Toxoplasma tissue cysts. After that periodic acid Schiff (PAS) stain confirmed that only three samples (3%) were Toxoplasma. Finally, enzyme-linked immunosorbent assay (ELISA) asserted that those three samples were Toxoplasma gondii. This study provides significant evidence about risk of human exposure to Toxoplasma through the consumption of raw or undercooked buffalo meat potentially contaminated with infectious tissue cysts.","PeriodicalId":7928,"journal":{"name":"alexandria journal of veterinary sciences","volume":"48 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"alexandria journal of veterinary sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5455/ajvs.148101","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Since buffalo meat, has been demonstrated to be a potential source of human infection, a careful evaluation of the prevalence of Toxoplasma infection in this meat is needed. Tissue cysts of Toxoplasma gondii are frequently found in the skeletal muscles of buffaloes. This study evaluated the prevalence of Toxoplasma gondii in the meat juice of buffalo meat samples via several diagnostic techniques to protect public health. Peptic digestion, histopathology, and serology were performed on meat juice from 100 buffalo meat samples from local butchers and retail beef markets. Eighteen samples (18%) were suspected of the presence of bradyzoites after digestion, and were subjected to histopathology which illustrated that only six samples (6%) were suspected to be Toxoplasma tissue cysts. After that periodic acid Schiff (PAS) stain confirmed that only three samples (3%) were Toxoplasma. Finally, enzyme-linked immunosorbent assay (ELISA) asserted that those three samples were Toxoplasma gondii. This study provides significant evidence about risk of human exposure to Toxoplasma through the consumption of raw or undercooked buffalo meat potentially contaminated with infectious tissue cysts.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
水牛肉中弓形虫的研究进展
由于水牛肉已被证明是人类感染的潜在来源,因此需要仔细评估这种肉类中弓形虫感染的流行程度。刚地弓形虫的组织囊肿经常在水牛的骨骼肌中发现。本研究通过几种诊断技术评估了刚地弓形虫在水牛肉样品肉汁中的流行程度,以保护公众健康。对来自当地肉店和零售牛肉市场的100份水牛肉样本的肉汁进行了消化、组织病理学和血清学检测。18个样本(18%)在消化后怀疑存在慢殖子,并进行组织病理学检查,结果显示只有6个样本(6%)怀疑是弓形虫组织囊肿。之后,周期性酸性希夫(PAS)染色证实只有三个样本(3%)是弓形虫。最后,酶联免疫吸附试验(ELISA)确认这3份样品为刚地弓形虫。这项研究提供了重要的证据,证明人类通过食用可能被传染性组织囊肿污染的生的或未煮熟的水牛肉而暴露于弓形虫的风险。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Impact of Crossbreeding Between Local and Foreign Strains on the Growth Traits and the Expression of Insulin-Like Growth Factor Genes on Their Chicks Biochemical Changes of Mancozeb-Induced Alternations in Testes and Thyroid gland of Male Albino Rats Effect of DL Methionine Supplementation in Japanese Quail Diets on Growth, Meat Quality and Health Detection of uidA, stx1, and stx2 Genes in Escherichia coli O157:H7 Isolated from Cattle Faecal Matter and River Water Pasteurella multocida Infection in Duck, Detection of Virulence Genes and Antimicrobial Susceptibility of the Isolates
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1