The Promoter Hypermethylation of DAPK Gene and p16 Gene in Non-Small Cell Lung Cancer Patients

2009 2nd International Conference on Biomedical Engineering and Informatics Pub Date : 2009-10-30 DOI:10.1109/BMEI.2009.5305423

Shi-ying Zheng, D. Jiang, J. Ge

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引用次数: 1

Abstract

bjective�÷ To evaluate the clinical significan ce of the aberrant methylation of DAPK gene and p16 gene in serum from 65 NSCLC patients from the first affiliated hospital of suzhou university� Mothods �÷ A methylation-specific PCR (MSP)was performed for the detection of promoter hypermethylation of DAPK gene and p16 gene in blood DNA from 65 cases of NSCLC� and to analyze the relation of the aberrant methylation of DAPK gene and p16 gene and the clinicopathological data� Results�÷ 30.8�i (20�A65)of the sera from 65 cases of NSCLC showed hypermethylation for DAPK promoter and 43.1�i (28�A 65)the same for p16 promoter�» whereas no methylated DAPK gene promoter and p16 gene promoter were found in sera from the patients with lung benign diseases and normal controls� Methylated DAPK gene promoter and p16 gene promoter in sera were not closely correlated with the pathological classification �» stage �»metastas is and differentiation in NSCLC� Conclusion�÷ Detection of the aberrant methylation of DAPK gene and p16 gene in blood DNA from NSCLC patients might offer an effective meaa-lS for the earlier auxiliar y diagnosis of the malignancy�

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非小细胞肺癌患者DAPK基因和p16基因启动子超甲基化的研究

bjective�÷评估的临床significan ce DAPK基因的异常甲基化和p16基因从65名非小细胞肺癌患者血清从苏州大学第一附属医院�方法�÷进行聚合酶链反应(MSP) methylation-specific DAPK基因启动子甲基化的检测和p16基因在血液DNA从65例NSCLC�和分析DAPK基因的异常甲基化的关系和p16基因和临床病理的数据结果��÷30.8�我(20�A65)的65例非小细胞肺癌血清显示为DAPK启动子甲基化和43.1�我对于p16(28�65)相同的启动子�»而没有和p16基因甲基化DAPK基因启动子中发现血清肺良性疾病患者和正常对照组�和p16基因甲基化DAPK基因启动子血清并不密切相关的病理分类��»»阶段结论metastas和分化在NSCLC��÷异常的检测非小细胞肺癌患者血液DNA中DAPK基因和p16基因的甲基化可能为恶性肿瘤的早期辅助诊断提供一种有效的手段

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2009 2nd International Conference on Biomedical Engineering and Informatics

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