{"title":"DEVELOPMENT OF FLUORESCENCE POLARIZATION IMMUNOASSAY OF PHTHALATES: COMPARISON OF DIFFERENT DERIVATIVES IN OBTAINING ANTIBODIES AND TRACERS","authors":"I. A. Shanin, M. Karimova, L. Mukhametova","doi":"10.37747/2312-640x-2021-19-232-234","DOIUrl":null,"url":null,"abstract":"Reagents for fluorescence polarization immunoassay (FPIA) of phthalates have been obtained: phthalate-protein conjugates, polyclonal antibodies, tracers - antigens labeled with fluorescein derivatives. By the example of dibutyl phthalate (DBP) with the use of a portable detector Sentry-200, the FPIA method was developed, which makes it possible to determine DBP in water samples in concentrations 1 μg / ml in 5 min.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"51 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"http://eng.biomos.ru/conference/articles.htm","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.37747/2312-640x-2021-19-232-234","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Reagents for fluorescence polarization immunoassay (FPIA) of phthalates have been obtained: phthalate-protein conjugates, polyclonal antibodies, tracers - antigens labeled with fluorescein derivatives. By the example of dibutyl phthalate (DBP) with the use of a portable detector Sentry-200, the FPIA method was developed, which makes it possible to determine DBP in water samples in concentrations 1 μg / ml in 5 min.
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