G. S. Djarkasi, Lana E. Lalujan, Erny J. N. Nurali, M. Sumual
{"title":"Antioxidant activity of karimenga (Acorus calamus)","authors":"G. S. Djarkasi, Lana E. Lalujan, Erny J. N. Nurali, M. Sumual","doi":"10.1063/1.5125555","DOIUrl":null,"url":null,"abstract":"Karimenga (Acorus calamus) is an underutilized plant that contains bioactive compounds such as phenolics, flavonoids, and terpenoids. The accumulation of phenolic compounds varies in each part of the plant; hence the phenolic compounds in this experiment were extracted from leaf and rhizome using different organic solvents. The antioxidant activity was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that total phenol from the leaf extracted by methanol, acetone, ethyl acetate, and hexane were 22.17 mg GAE/g, 15.89 mg GAE /g, 12.79 mg GAE /g, and 11.49 mg GAE/g respectively. The flavonoid content were 5.07 mg QE /g, 6.55 mg QE /g, 3.85 mg QE /g, and 2.23 mg QE /g respectively which were calculated base on quercetin standard. On the other hand, the total phenol and flavonoid content from karimenga rhizome extracted by each organic solvent were 240.32 mg GAE/g, 139.02 mg GAE/g, 99.63 mg GAE/g, 105.57 mg GAE/g, and 63.06 mg QE /g, 48.35 mg QE /g, 43.07 mg QE /g, 31.78 mg QE /g, respectively. The antioxidant activities of leaf and rhizome extracts expressed as free radical scavenging activity were in the range of 75 – 90 %. The main volatile compound detected by GC-MS was α- and β-asarone.Karimenga (Acorus calamus) is an underutilized plant that contains bioactive compounds such as phenolics, flavonoids, and terpenoids. The accumulation of phenolic compounds varies in each part of the plant; hence the phenolic compounds in this experiment were extracted from leaf and rhizome using different organic solvents. The antioxidant activity was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that total phenol from the leaf extracted by methanol, acetone, ethyl acetate, and hexane were 22.17 mg GAE/g, 15.89 mg GAE /g, 12.79 mg GAE /g, and 11.49 mg GAE/g respectively. The flavonoid content were 5.07 mg QE /g, 6.55 mg QE /g, 3.85 mg QE /g, and 2.23 mg QE /g respectively which were calculated base on quercetin standard. On the other hand, the total phenol and flavonoid content from karimenga rhizome extracted by each organic solvent were 240.32 mg GAE/g, 139.02 mg GAE/g, 99.63 mg GAE/g, 105.57 mg GAE/g, and 63.06 mg QE /g, 48.35 mg QE /g, 43.07 mg QE /g, 31.78 mg QE /g,...","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":"51 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1063/1.5125555","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
Abstract
Karimenga (Acorus calamus) is an underutilized plant that contains bioactive compounds such as phenolics, flavonoids, and terpenoids. The accumulation of phenolic compounds varies in each part of the plant; hence the phenolic compounds in this experiment were extracted from leaf and rhizome using different organic solvents. The antioxidant activity was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that total phenol from the leaf extracted by methanol, acetone, ethyl acetate, and hexane were 22.17 mg GAE/g, 15.89 mg GAE /g, 12.79 mg GAE /g, and 11.49 mg GAE/g respectively. The flavonoid content were 5.07 mg QE /g, 6.55 mg QE /g, 3.85 mg QE /g, and 2.23 mg QE /g respectively which were calculated base on quercetin standard. On the other hand, the total phenol and flavonoid content from karimenga rhizome extracted by each organic solvent were 240.32 mg GAE/g, 139.02 mg GAE/g, 99.63 mg GAE/g, 105.57 mg GAE/g, and 63.06 mg QE /g, 48.35 mg QE /g, 43.07 mg QE /g, 31.78 mg QE /g, respectively. The antioxidant activities of leaf and rhizome extracts expressed as free radical scavenging activity were in the range of 75 – 90 %. The main volatile compound detected by GC-MS was α- and β-asarone.Karimenga (Acorus calamus) is an underutilized plant that contains bioactive compounds such as phenolics, flavonoids, and terpenoids. The accumulation of phenolic compounds varies in each part of the plant; hence the phenolic compounds in this experiment were extracted from leaf and rhizome using different organic solvents. The antioxidant activity was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that total phenol from the leaf extracted by methanol, acetone, ethyl acetate, and hexane were 22.17 mg GAE/g, 15.89 mg GAE /g, 12.79 mg GAE /g, and 11.49 mg GAE/g respectively. The flavonoid content were 5.07 mg QE /g, 6.55 mg QE /g, 3.85 mg QE /g, and 2.23 mg QE /g respectively which were calculated base on quercetin standard. On the other hand, the total phenol and flavonoid content from karimenga rhizome extracted by each organic solvent were 240.32 mg GAE/g, 139.02 mg GAE/g, 99.63 mg GAE/g, 105.57 mg GAE/g, and 63.06 mg QE /g, 48.35 mg QE /g, 43.07 mg QE /g, 31.78 mg QE /g,...