Effect of Force Field On Deformation and Migration of Single Cell with Orientation Controlled by Micro-striped Topography Patterns

S. Hashimoto, Kazuya Kishimoto
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Abstract

The effect of the force field parallel to the surface of the scaffold plate on cell activity (deformation, migration) has been studied in vitro. Cell orientation was aligned by microtopography patterns (striped ridgelines; height 0.7 μm, width 3 μm, spacing 3 μm) on the scaffold plane. Three compartments were designed with different angles (0 degrees, 45 degrees, and 90 degrees) between the longitudinal direction of the ridge and the direction of the force field. Osteoblasts (MC3T3-E1; osteoblast precursor cell line derived from mouse calvaria) were used in the experiment. After applying a force field for 5 hours, the position and contour of each cell were tracked on time-lapse microscopy images for 48 hours. Experimental results show that force fields not parallel to the major axis of the cell activate subsequent actions (deformation, migration) of the cell. The effect is maintained for 48 hours even if the cells move to different areas of the topography pattern.
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力场对微条纹形貌控制取向的单细胞变形和迁移的影响
体外实验研究了平行于支架板表面的力场对细胞活性(变形、迁移)的影响。微地形模式(条纹脊线;脚手架平面高度0.7 μm,宽度3 μm,间距3 μm。在脊线纵向与力场方向之间设计了3个不同角度(0度、45度和90度)的隔室。成骨细胞(MC3T3-E1;实验采用从小鼠颅骨中提取的成骨前体细胞。施加力场5小时后,在延时显微镜图像上追踪每个细胞的位置和轮廓48小时。实验结果表明,不平行于细胞长轴的力场会激活细胞的后续动作(变形、迁移)。即使细胞移动到地形模式的不同区域,这种效果也能维持48小时。
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