Effect of salvia miltiorrhiza and ligustrazine injection on P-selectin and tissue factor in rats of pulmonary embolism

Abstract

Objective To study the effects of salvia miltiorrhiza and ligustrazine injection, low molecular weight heparin and their combination on pulmonary embolism rats by observing the changes of P-selectin (CD62P) and tissue factor (TF) in the blood of rats of pulmonary embolism. Methods 80 healthy male Wistar rats were randomly divided into 7 h and 7 d groups.Each group was divided into control group, embolism group, salviae miltiorrhiza and ligustrazine group, low molecular weight heparin group, and combined drug group, each group with eight rats.The rats were separated from the right jugular vein, the control group was injected with saline, and the remaining groups were injected with homemade emboli (n=25±3, d=0.5 mm). Each group collected blood at the 7th hour and the 7th day, TF and CD62P concentrations were detected, and lung tissue was observed. Results After embolization, rats had panting, cyanosis, accelerated heart rate, etc.The surface of lung tissue was rugged, uneven expansion, scattered in gray-white infarction areas.Compared with the control group, the concentrations of TF and CD62P in plasma of the embolism group increased significantly (all P<0.05). Compared with the embolism group, the concentrations of TF and CD62P of the therapy groups decreased to different degrees, and the combined group after therapy for one week was most significant. Conclusions The ideal model of pulmonary embolism can be prepared by jugular vein injection allothrombosis.The levels of TF and CD62P in pulmonary embolism rats could be significantly reduced by the combination of salvia miltiorrhiza and ligustrazine and low molecular weight heparin. Key words: Pulmonary embolism; Salvia miltiorrhiza and ligustrazine; Thromboplastin; P-selectin