Ochratoxin A – Bestimmung von Ochratoxin A in Serum mittels CE-LIF. Biomonitoring-Methode

Abstract

The working group “Analyses in Biological Materials” of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area developed the presented biomonitoring method. The method enables the determination of ochratoxin A in small volumes of serum. The samples are purified by liquid-liquid extraction and the analyte is extracted from the acidified sample with dichloromethane. After the extraction agent has been removed, the residue is dissolved in water. The extracts are injected into the capillary, then the individual components are separated by capillary electrophoresis and detected by laser-induced fluorescence. Coumarin-3-carboxylic acid is used as an internal standard. Calibration standards are prepared in serum and processed in the same way as the samples to be analysed. The MAK Collection for Occupational Health and Safety 2020, Vol 5, No 4 1 Biomonitoring-Methoden – Ochratoxin A in Serum 1 Kenndaten der Methode Matrix Serum Analytisches Messprinzip Kapillarelektrophorese mit laserinduzierter Fluoreszenzdetektion (CE-LIF) Parameter und entsprechender Arbeitsstoff Arbeitsstoff CAS-Nr. Parameter CAS-Nr. Ochratoxin A 303-47‐9 Ochratoxin A 303-47‐9 Zuverlässigkeitskriterien