{"title":"DEVELOPMENT OF A PRODUCER OF RECOMBINANT MARAL CHYMOSIN BASED ON THE YEAST KLUYVEROMYCES LACTIS","authors":"S. Belenkaya, V. V. Elchaninov","doi":"10.37747/2312-640x-2021-19-331-332","DOIUrl":null,"url":null,"abstract":"A plasmid vector pSVB was developed, which allows integrating an expression cassette containing the maral prochymosin gene into the genome of the yeast Kluyveromyces lactis. Producer strains have been obtained that ensure the accumulation of recombinant maral chymosin in the culture medium with a milk-clotting activity of up to 145 AU / ml.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"5 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"http://eng.biomos.ru/conference/articles.htm","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.37747/2312-640x-2021-19-331-332","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
A plasmid vector pSVB was developed, which allows integrating an expression cassette containing the maral prochymosin gene into the genome of the yeast Kluyveromyces lactis. Producer strains have been obtained that ensure the accumulation of recombinant maral chymosin in the culture medium with a milk-clotting activity of up to 145 AU / ml.
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