Study of oncolytic enzyme production from marine Streptomyces krainskii VMB-11

Abstract

L-asparaginase is an important enzyme, which plays a significant role in the treatment of acute lymphoblastic leukemia, acute myelocytic leukemia, and other carcinoma treatments. It is well known as an anti-cancerous agent because of its stability in varied environmental conditions. Streptomyces sp. is occupied the first position for the producing L-asparaginase from marine and terrestrial sources. In the presence of L-asparaginase, tumor cells get deprived and can’t endure. It acts as a potent antitumor or anti-leukemic drug. Because of the urgent need, an attempt was made for isolation and optimization of marine actinomycetes for the production of L-asparaginase. S. krainskii VMB-11 was isolated and inoculated in optimized M-9 media at 40 ° C, 96 h improve the production of L-asparaginase from 8.2 IU to 13.2 IU/g dry wt.of biomass. To the simplest of our data, this is often the primary report on production, purification, and characterization of L-asparaginase from marine Streptomyces isolate VMB-11 strain.