Evaluation of sample pooling method for SARS CoV-2 RNA detection; a laboratory based simulated study

P. A. S. L. Wijesuriya, M. Muthugala, H. Herath
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Abstract

Real time RT-PCR is considered as the gold standard test to detect COVID-19. The use of sample pooling strategy increases testing capacity and spares resources. However, the effectiveness of sample pooling should be evaluated in the setting before being implemented. Forty five samples including 20 high positives (Ct<20), 20 low positives (Ct 20-40) and 05 negative samples were used to prepare 1:1, 1:3 and 1:5 simulated sample pools which were then subjected to viral RNA extraction followed by real time RT-PCR. Sensitivity and specificity of sample pooling technique in the detection of SARS-CoV-2 RNA was 100% without significant variation of Ct values. According to our results, pooling of up to 6 samples will not have an effect on the final result in clinical samples and hence can be adopted in the given context for the diagnosis of COVID-19 by RT-PCR.
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SARS CoV-2 RNA检测的样本池法评价基于实验室的模拟研究
实时RT-PCR被认为是检测新冠病毒的金标准。使用样本池策略增加了测试能力并节省了资源。然而,在实施之前,应该在环境中评估样本汇集的有效性。选取高阳性20份(Ct<20)、低阳性20份(Ct 20-40)和阴性05份,共45份样品,分别制备1:1、1:3和1:5模拟样品池,提取病毒RNA,进行实时RT-PCR。样本池技术检测SARS-CoV-2 RNA的灵敏度和特异性均为100%,Ct值无明显变化。根据我们的研究结果,最多6个样本的汇集不会对临床样本的最终结果产生影响,因此可以在给定的背景下采用RT-PCR诊断COVID-19。
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审稿时长
16 weeks
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