Changes in biochemical parameters during the solid-state fermentation of pineapple (Ananas comosus I. Merr) peels by Rhizopus oligosporus

A. Anigboro, Egosmaka O. Egbune, O. Akeghware, F.O. Ovowa, N. Anyanwu, C. Chebene, C. Ogbeyi, N. J. Tonukari
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Abstract

Enzymes production and other relevant biomolecules from environmental wastes could be easily enhanced or hindered by slight alterations in the bioprocess. In this study, the impact of pH variations on amylase production by Rhizopus oligosporus during the solid state fermentation of pineapple (Ananas comosus L. Merr) peels was investigated. After five days of fermentation, the variations in glucose and soluble protein levels of the fermented pineapple peels were also investigated. The results established a notable increase (p<0.05) in the levels of soluble proteins and glucose in the fermented pineapple peels at all the pH values tested (pH 3 to 9); however, while they were highest at pH 3, amylase activity was highest at pH 6. This preliminary study shows that solid state fermented pineapple peels are rich in glucose, soluble proteins and amylase.
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少孢根霉固态发酵菠萝果皮过程中生化参数的变化
从环境废物中产生的酶和其他相关生物分子可以很容易地通过生物过程的轻微改变来增强或阻碍。在本研究中,研究了pH变化对菠萝果皮固态发酵过程中少孢根霉淀粉酶产量的影响。发酵5天后,还研究了发酵菠萝皮中葡萄糖和可溶性蛋白水平的变化。结果表明,在pH值3 ~ 9范围内,发酵菠萝皮中可溶性蛋白和葡萄糖含量均显著升高(p<0.05);pH值为3时淀粉酶活性最高,pH值为6时淀粉酶活性最高。初步研究表明,菠萝果皮固体发酵产物中含有丰富的葡萄糖、可溶性蛋白和淀粉酶。
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