Investigation regarding the potential application of grape pomace extracts on in vitro plant growth and development

IF 1 Q4 CHEMISTRY, MULTIDISCIPLINARY Ovidius University Annals of Chemistry Pub Date : 2022-07-01 DOI:10.2478/auoc-2022-0020

Petronela Elena Bran, Daniela Nicuţă, L. Grosu, Oana-Irina Patriciu, I. Alexa

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引用次数: 1

Abstract

Abstract The grape pomace hydroalcoholic extracts obtained by two different extraction methods were tested for biostimulatory potential activity for Origanum vulgare L. cultures. The total polyphenols contents of extracts were evaluated by Folin-Ciocalteu method. Characteristics such as: pH, salinity, conductivity and total dissolved solids were determined. FTIR and UV spectra of extracts were also recorded. The effect of grape pomace extracts on growth and development of oregano plant were studied by carrying out in vitro propagation of oregano on Murashige and Skoog basal medium supplemented with different concentrations of grape pomace extracts. Biometric measurements, growth rate and biomass accumulation have been narrowly monitored for all samples and compared to the control sample. The results have shown that the morphogenetic response depends both on the proportion in which basal medium was supplemented and on the extraction method used. A stimulation of growth and development at a low concentration of grape pomace extracts has been noted. The best results were observed in the samples with 1 and 5 % of grape pomace extract obtained by classical extraction.

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葡萄渣提取物在离体植物生长发育中的潜在应用研究

摘要采用两种不同提取方法对葡萄渣水醇提取物进行了生物刺激活性测定。采用Folin-Ciocalteu法测定提取物中总多酚的含量。测定了样品的pH、盐度、电导率和总溶解固形物等特性。并记录了提取物的红外光谱和紫外光谱。通过在添加不同浓度葡萄渣提取物的Murashige和Skoog基础培养基上对牛至进行离体繁殖，研究了葡萄渣提取物对牛至植株生长发育的影响。对所有样品的生物测量、生长速率和生物量积累进行了严格监测，并与对照样品进行了比较。结果表明，形态发生反应既取决于基础培养基的添加比例，也取决于提取方法。已经注意到低浓度的葡萄渣提取物对生长发育的刺激。以经典提取法得到的葡萄渣提取物含量为1%和5%的样品效果最佳。

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Ovidius University Annals of Chemistry CHEMISTRY, MULTIDISCIPLINARY-

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5 weeks