The Role of Drought Stress on Aflatoxin Contaminationin Groundnuts (Arachis hypogea L.) and Aspergillusflavus Population in the Soil

Abstract

Aflatoxins are naturally occurring toxic chemical substances that are produced by fungal species called Aspergillus flavus. The toxic substances are secondary metabolites, which contaminate groundnut while growing in the field and also post-harvest. Drought stress is one of the factors that contribute to increased aflatoxin levels in groundnut during field production. This study was conducted in a screen house at ICRISAT-Chitedze Agricultural Research Station, Malawi to investigate the effects of drought on aflatoxin contamination and A.flavus population in the soil. Four drought stress levels; prolonged (4 weeks), min (3 weeks), mild (2 weeks) and no drought were imposed on five groundnut varieties at pod filling stage. Soil samples were collected from each plot four times; at planting, beginning of drought, end of drought and at harvest. Aflatoxin levels in groundnut grain samples were estimated by use of neogenstrips read with mobile assay tablet reader. Population densities of A.flavus in soil samples collected from the plots were estimated using serial dilutions plated on the selective media, modified dichloran Rose Bengal (MDRB) and quantify A.flavus within 3 days after incubation at 37°C.The results showed that there were significant differences in aflatoxin contamination between drought stress levels (p = 0.011). High aflatoxin contamination was observed under prolonged drought (22.0 ppb) compared to and no drought treatment (1.5 ppb). None of the varieties used in the study showed either resistance or susceptibility to aflatoxin contamination under drought or adequate soil moisture. The results also showed that there were significant differences in A.flavus population at drought period and harvesting time and the mean population of A.flavus in prolonged drought at end of stress and harvesting were 8511 and 6044 cfu/g of soil respectively. It was concluded that drought contribute to aflatoxin contamination in groundnut, and also increased the A.flavus population in soil and also at harvesting.