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Effect of Adding Fiber from SeraiKayu (Eugenia Polyantha) Waste on Organoleptic Characteristic and Sensory Evaluation of Cracker 三来玉废纤维的添加对饼干感官特性及感官评价的影响
Pub Date : 2017-12-20 DOI: 10.15341/MAST(2375-9402)/03.03.2017/002
I. Abdullah, N. Wahab, H. M. Hassan
The effect of adding fiber from SeraiKayu (Eugenia polyantha) waste in cracker was studied on organoleptic characteristic and sensory evaluation of cracker. Cracker prepared from SeraiKayu (Eugenia polyantha) waste containing different proportion of waste (2%, 4% and 6%) from two sources of waste, boiling and extraction of SeraiKayu (Eugenia polyantha) leaves. The crackers were evaluated for organoleptic characteristics and sensory evaluation. Statistical analysis revealed no significant differences (P <0.05) among formulations of the crackers for colour, firmness, saltiness and crunchiness characteristics. Meanwhile, the bitterness and aroma characteristics of the crackers were affected significantly with increasing levels of SeraiKayu (Eugenia polyantha) waste in crackers. In general, the crackers were accepted by the panelist during the sensory evaluation test and has potential as new source of fiber for use in high fiber products.
研究了在裂化剂中添加七来玉废纤维对裂化剂感官特性和感官评价的影响。以含不同比例废弃物(2%、4%和6%)的白玉(Eugenia polyantha)废弃物为原料,对白玉(Eugenia polyantha)叶片进行煮沸和提取,制备出白玉(Eugenia polyantha)废弃物。对饼干的感官特性和感官评价进行了评价。不同配方的饼干颜色、硬度、咸度和脆度差异均不显著(P <0.05)。同时,随着白刺废弃物含量的增加,白刺的苦味和香气特性也受到显著影响。总的来说,在感官评价试验中,裂片被小组成员所接受,具有作为高纤维产品的新纤维来源的潜力。
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引用次数: 1
Screening of Lactic Acid Bacteria from “TapaiPulut” for Biosynthesis of Glutamic Acid 从“TapaiPulut”中筛选生物合成谷氨酸的乳酸菌
Pub Date : 2017-12-20 DOI: 10.15341/mast(2375-9402)/03.03.2017/007
Mohd Syafarim Md Ishak, M. Ibrahim, H. Ishak
Glutamic acid (glutamate) is an amino acid used in human body to build proteins. Glutamate can be converted to glutamine via glutamine synthetase, and to γ — aminobutyric acid (GABA) via glutamate decarboxylase. The objective of this study is to screen potential of lactic acid bacteria from “tapaiubi” for biosynthesis of glutamic acid. The traditional fermented food, “tapaipulut” was used as a source of lactic acid bacteria (LAB). The sample was cultivated on DeMan-Rogosa-Sharpe MRS agar containing 0.16% bromocresol purple and placed under anaerobic condition at 37°C for 48 hours. A total of 25 strains were isolated with 8 strains were determined to be LAB based on their morphology. The 8 strains were inoculated into 8 bottles of fermentation medium (4% v/v) in duplicate determinations and fermented in rotary shaker (180 rpm, 37°C, 4 days). After 18 hours, the fermented media were centrifuged at 10000 rpm (4°C) for 10 minutes and the supernatants were derivatised at 100°C for 10 minutes. Glutamic acid concentrations were determined by using HPLC with fluorescence detector (C18S) on a 250 × 4.6 mm column at a flow rate of 1.2 ml/min. Cut of 25 strains, 8 strains were shown to have the capability of producing glutamic acid. Five strains, including (TUS 25 (18.409 μg/ml), TUS 17 (18.437 μg/ml), TUS 7 (17.228 μg/ml), TUS 8 (14.033 μg/ml), TUS 3 (7.810 μg/ml) showed the capability of producing glutamic acid after 72 hours fermentation. While the other three strains TUS 11 (4.894 μg/ml), TUS 2 (0.0245 μg/ml), TUS 20 (0.405 μg/ml) were become 48 hours and 72 hours fermentation. The highest glutamate was registered after 48 hours and 96 hours. This study shows that LAB from “tapaipulut” has the capability of producing glutamic acid.
谷氨酸(谷氨酸)是一种在人体中用于构建蛋白质的氨基酸。谷氨酸可通过谷氨酰胺合成酶转化为谷氨酰胺,通过谷氨酸脱羧酶转化为γ -氨基丁酸(GABA)。本研究的目的是筛选从“塔帕乌比”中提取的乳酸菌用于生物合成谷氨酸的潜力。利用传统发酵食品“tapaipulut”作为乳酸菌的来源。样品在含0.16%溴甲酚紫的dem - rogosa - sharpe MRS琼脂上培养,在37℃厌氧条件下培养48小时。共分离菌株25株,经形态学鉴定为LAB菌株8株。将8株菌株接种于8瓶发酵培养基(4% v/v)中,重复测定,在旋转摇床(180 rpm, 37℃,4 d)中发酵。18小时后,发酵培养基在10000 rpm(4℃)下离心10分钟,上清液在100℃下衍生10分钟。采用高效液相色谱法测定谷氨酸浓度,色谱柱为250 × 4.6 mm,流速为1.2 ml/min,荧光检测器(C18S)。从25株菌株中筛选出8株具有生产谷氨酸的能力。5株菌株(TUS 25 (18.409 μg/ml)、TUS 17 (18.437 μg/ml)、TUS 7 (17.228 μg/ml)、TUS 8 (14.033 μg/ml)、TUS 3 (7.810 μg/ml)在发酵72 h后显示出产谷氨酸的能力。其余3株菌株TUS 11 (4.894 μg/ml)、TUS 2 (0.0245 μg/ml)、TUS 20 (0.405 μg/ml)分别发酵48 h和72 h。谷氨酸在48 h和96 h时最高。本研究表明,从“tapaipulut”中提取的乳酸菌具有生产谷氨酸的能力。
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引用次数: 0
The Role of Drought Stress on Aflatoxin Contaminationin Groundnuts (Arachis hypogea L.) and Aspergillusflavus Population in the Soil 干旱胁迫对花生黄曲霉毒素污染及土壤黄曲霉种群的影响
Pub Date : 2017-12-20 DOI: 10.15341/MAST(2375-9402)/03.03.2017/005
C. B. Sibakwe, T. Kasambara-Donga, S. Njoroge, W. Msuku, W. G. Mhang, R. Brandenburg, D. Jordan
Aflatoxins are naturally occurring toxic chemical substances that are produced by fungal species called Aspergillus flavus. The toxic substances are secondary metabolites, which contaminate groundnut while growing in the field and also post-harvest. Drought stress is one of the factors that contribute to increased aflatoxin levels in groundnut during field production. This study was conducted in a screen house at ICRISAT-Chitedze Agricultural Research Station, Malawi to investigate the effects of drought on aflatoxin contamination and A.flavus population in the soil. Four drought stress levels; prolonged (4 weeks), min (3 weeks), mild (2 weeks) and no drought were imposed on five groundnut varieties at pod filling stage. Soil samples were collected from each plot four times; at planting, beginning of drought, end of drought and at harvest. Aflatoxin levels in groundnut grain samples were estimated by use of neogenstrips read with mobile assay tablet reader. Population densities of A.flavus in soil samples collected from the plots were estimated using serial dilutions plated on the selective media, modified dichloran Rose Bengal (MDRB) and quantify A.flavus within 3 days after incubation at 37°C.The results showed that there were significant differences in aflatoxin contamination between drought stress levels (p = 0.011). High aflatoxin contamination was observed under prolonged drought (22.0 ppb) compared to and no drought treatment (1.5 ppb). None of the varieties used in the study showed either resistance or susceptibility to aflatoxin contamination under drought or adequate soil moisture. The results also showed that there were significant differences in A.flavus population at drought period and harvesting time and the mean population of A.flavus in prolonged drought at end of stress and harvesting were 8511 and 6044 cfu/g of soil respectively. It was concluded that drought contribute to aflatoxin contamination in groundnut, and also increased the A.flavus population in soil and also at harvesting.
黄曲霉毒素是一种天然存在的有毒化学物质,由一种叫做黄曲霉的真菌产生。这些有毒物质是次生代谢物,在花生的田间生长和收获后都会对其造成污染。干旱胁迫是田间花生黄曲霉毒素含量升高的原因之一。本研究是在马拉维ICRISAT-Chitedze农业研究站的一个筛选室进行的,目的是调查干旱对土壤中黄曲霉毒素污染和黄曲霉种群的影响。4个干旱胁迫水平;灌荚期对5个花生品种进行了长时间(4周)、短时间(3周)、轻度(2周)和不干旱处理。每个地块采集4次土壤样本;在播种、干旱开始、干旱结束和收获时。花生颗粒样品中黄曲霉毒素的含量是用移动测定仪读取的neogen试纸来估计的。在37°C孵育后3天内,利用选择性培养基、改性双氯兰孟加拉玫瑰(MDRB)进行连续稀释,估计了从样地收集的土壤样品中黄曲霉的种群密度。结果表明,不同干旱胁迫水平黄曲霉毒素污染差异显著(p = 0.011)。与未干旱处理(1.5 ppb)相比,在长期干旱(22.0 ppb)下观察到高黄曲霉毒素污染。在干旱或土壤水分充足的情况下,研究中使用的品种都没有表现出对黄曲霉毒素污染的抗性或敏感性。干旱期和收获期黄曲霉种群数量差异显著,干旱期和收获期黄曲霉平均种群数量分别为8511 cfu/g和6044 cfu/g。结果表明,干旱是花生黄曲霉毒素污染的主要原因,也增加了土壤和收获时黄曲霉的数量。
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引用次数: 13
Phytochemicals Screening and Antioxidant Activity of Three Different Solvent Extracts of Euodia Redleyi Leaves 三种不同溶剂提取物的植物化学成分筛选及抗氧化活性研究
Pub Date : 2017-12-20 DOI: 10.15341/MAST(2375-9402)/03.03.2017/004
N. A. Maskam, N. Ravi, H. M. Hassan, M. M. Nor
This study was conducted to identify the phytochemicals screening and antioxidant activity of three different solvent extracts of Euodia Redleyi leaves. The sample was collected at Pahang, Malaysia. Hexane, dicholoromethane (DCM) and methanol extracts of the leaves were used in this study. The established conventional methods were used for quantify the total phenol, flavonoids contents and antioxidant activity. Phytochemical screening test determined the presence of flavonoids, tannin, saponin, terpenoid and phenolic content. Total antioxidant activities were determined by DPPH method and Total phenolic content (TPC) were determined by Follin-Ciocalteau method. A screening test for E.redleyi showed the presence of all phytochemical but absence of saponin. There was a significant difference between hexane, DCM and methanol plant extracts; hexane and DCM extracts were more prominent in antioxidant activity but methanol extraction was more prominent in TPC. Since E. redleyi exhibited high antioxidant and TPC; it can be used as a natural antioxidant. Further study needed to identify the exact active compound underlying this high antioxidant activity.
本研究对红迭叶三种不同溶剂提取物的化学成分筛选及抗氧化活性进行了研究。样本采集于马来西亚彭亨。采用己烷、二氯甲烷(DCM)和甲醇提取物进行研究。采用建立的常规方法定量测定其总酚、总黄酮含量和抗氧化活性。植物化学筛选试验测定了黄酮类化合物、单宁、皂苷、萜类化合物和酚类化合物的含量。DPPH法测定总抗氧化活性,Follin-Ciocalteau法测定总酚含量(TPC)。筛选结果表明,红毛霉含有所有植物化学成分,但不含皂苷。正己烷、DCM和甲醇植物提取物之间存在显著差异;己烷和DCM提取物的抗氧化活性较强,而甲醇提取物的抗氧化活性较强。由于红椒具有较高的抗氧化性和TPC;它可以作为天然抗氧化剂使用。需要进一步的研究来确定这种高抗氧化活性的确切活性化合物。
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引用次数: 1
Non-Excavation Subsurface Irrigation and Drainage Systemin the Reclaimed Lowland to be Cultivated with Upland-Field Crops 开垦低地种植旱地作物的非开挖地下灌溉排水系统
Pub Date : 2017-12-20 DOI: 10.15341/MAST(2375-9402)/03.03.2017/001
Hyuntai Kim, Donguk Seo, Jeonyong Ryu
Although previously the reclaimed land has been developed and used mainly for paddy field in Korea, there is a need to improve the reclaimed land to be cultivated for highland-field crop, due to the necessity for smooth management of grain supply and demand to cope with the changes of international and domestic agricultural environment, and higher farming income from highland-field crops than rice. However, it is difficult to cultivate highland-field crops in the reclaimed land, because it is mostly located in lowland, containing high salinity soil with a difficulty of drainage due to the characteristics of fine grained soil which is a major component of the reclaimed land. In addition, there is a big problem of re-salinization of root zone soil caused by the capillary rise of saline groundwater during the dry season. In this study, seepage analysis to draw high-capacity drainage system was conducted by each type of subsurface drainage system, and subsoil breaking and no-excavation subsurface drainage system were proposed to be utilized for the improvement of reclaimed land at low-cost to cultivate highland-field crops. Following results were acquired through pilot construction in the field. (1) Reclaimed soil of Korea, which is mostly impermeable (k < 1*10 cm/s), requires the introduction of (i) subsurface drain and (ii) subsoil breaking method, to improve the land to be cultivated for highland-field crop. (2) In order to array the appropriate spacing (3-10 m) by the soil type, it is necessary to develop and introduce cost-effective non-excavation subsurface drainage system installation method. (3) The introduction of subsurface irrigation and drainage system is necessary to clean drain system and to prevent re-salinization. (4) As a result of the pilot construction of cost-effective non-excavation subsurface irrigation and drainage system, it was confirmed that workability was improved due to the construction of non-excavation method, and construction cost was lower (75%) and subsurface drain and desalinization performance was far superior (over 150%) with 5 m intervals in parallel with subsoil breaking method than that of existing method with 10 m intervals. (5) It was proved that subsoil breaking and subsurface irrigation and drain system were efficient to clean the drain system using underground irrigation water as well as prevent re-salinization. And also it was confirmed that the system made the desalinization of soil from 10-15 ds/m to 2-5 ds/m within a year under the condition of natural rainfall possible. As the result of the crop cultivation on the pilot reclaimed land desalinized by subsoil breaking and subsurface irrigation and drain system, it was found that crop growth without any damage by moisture during wet season was shown in good status, and it led to conclusion that the system is highly effective for the development of reclaimed land.
虽然韩国以前开垦的土地主要用于水田的开发和利用,但由于应对国际国内农业环境的变化,需要对粮食供需进行顺畅的管理,并且高原作物的农业收入高于水稻,因此需要将开垦的土地改良为种植高原作物。然而,由于复垦土地多位于低地,土壤含盐量高,由于复垦土地主要成分为细粒土,排水困难,因此很难在复垦土地上种植高原作物。此外,旱季含盐地下水毛细上升导致根区土壤再盐渍化问题严重。本研究通过对各类地下排水系统进行渗流分析,抽取大容量排水系统,并提出利用地下破土和无开挖地下排水系统,以低成本改良复垦土地,种植高原作物。通过现场试验施工,取得了以下成果:(1)韩国的复垦土壤大部分是不透水的(k < 1*10 cm/s),需要引入(i)地下排水和(ii)底土破碎方法,以改善用于种植高原作物的土地。(2)为了按土壤类型排列合适的间距(3- 10m),有必要开发和引入具有成本效益的非开挖地下排水系统安装方法。(3)为清洁排水系统,防止再盐碱化,必须引进地下灌排系统。(4)经经济高效的非开挖地下灌排系统试点建设,证实了非开挖法施工可操作性提高,与现有10 m间隔的破底法并行,5 m间隔的施工成本低75%,地下排水脱盐性能远优于现有10 m间隔的方法(150%以上)。(5)试验证明,地下破土和地下灌排系统能有效地利用地下灌溉水清洁排水系统,防止再盐渍化。在自然降雨条件下,该系统可使土壤在1年内由10-15 ds/m脱盐至2-5 ds/m脱盐。在底土破碎脱盐和地下灌排系统的复垦土地上进行作物种植试验,发现作物在雨季不受水分损害的情况下生长状况良好,表明该系统对复垦土地的开发是非常有效的。
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引用次数: 0
The Effects of Dietary Crude Protein Levels on Nutrient Digestibility, Nitrogen Retention, Rumen Environment and Microbial Nitrogen Synthesis of Growing Female Bach Thao Goats in Vietnam 饲粮粗蛋白质水平对越南生长母羊营养物质消化率、氮沉积、瘤胃环境和微生物氮合成的影响
Pub Date : 2017-12-20 DOI: 10.15341/MAST(2375-9402)/03.03.2017/006
N. Thu
An experiment was carried out to evaluate the effects of crude protein (CP) contents on feed intake, nutrient digestibility, rumen parameters and estimated nitrogen synthesis of Bach Thao goats. Five female goats from 4-5 months of age (16.7±0.92 kg) were used for this experiment in a Latin square design. Five treatments were applied, containing dietary CP contents of 5.0, 5.5, 6.0, 6.5 or 7.0 g/kgLW/day. The results showed that dry matter (DM), Neutral detergent fiber (NDF) and acid detergent fiber (ADF) intakes and digestibilities were not significantly different among the treatments (P > 0.05), and the rumen parameters were also similar in different treatments. However, the nitrogen retention was significantly different (P < 0.05) among the treatments and it was higher for the treatments of 6.0, 6.5 and 7.0 gCP/kgLW. The purine derivatives and estimated microbial nitrogen synthesis were numerically higher for the treatments containing 6.0, 6.5 and 7.0 gCP/kgLW. Although there was a gradual increase of daily weight gain by increasing the amount of dietary CP from 5.0 to 7.0 gCP, no significant difference was found. Based on the results, the dietary CP from 6.0 to 6.5 g/kgLW for growing Bach Thao goats is recommended.
本试验旨在研究粗蛋白质(CP)含量对巴洮山羊采食量、营养物质消化率、瘤胃参数及估算氮合成的影响。试验选用4 ~ 5月龄(16.7±0.92 kg)的母山羊5只,采用拉丁方设计。试验采用5个处理,饲粮粗蛋白质含量分别为5.0、5.5、6.0、6.5和7.0 g/kgLW/day。结果表明:各处理间干物质(DM)、中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)采食量和消化率差异不显著(P > 0.05),瘤胃参数相似。但各处理间氮滞留量差异显著(P < 0.05),以6.0、6.5和7.0 gCP/kgLW处理最高。6.0、6.5和7.0 gCP/kgLW处理的嘌呤衍生物和微生物氮合成估计值较高。饲粮粗蛋白质从5.0 gCP增加到7.0 gCP,日增重逐渐增加,但差异不显著。综上所述,巴陶山羊生长期饲粮粗蛋白质推荐水平为6.0 ~ 6.5 g/kgLW。
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引用次数: 0
An Investigation on Tilapia Culture in Aquaponic System in Iran 伊朗水培系统罗非鱼养殖研究
Pub Date : 2017-12-20 DOI: 10.15341/MAST(2375-9402)/03.03.2017/003
F. Rajabipour, N. Mashaii, H. Sarsangi, M. Mohammadi, A. Matinfar
Water shortage and deficit of agricultural soil are limiting agents for development of agronomy, especially in dry areas, such as Iran. Tilapia is an important commercial fish because of its fast growth, tolerance to environmental conditions and diseases, possibility of intensive culture, low costs of production and marketable flavor. Production of aquatic and agricultural crops in an aquaponic system would ask these expectations. Aquaponic system that includes combination of hydroponics and water recycling systems was examined for modulated culture of tilapia and agricultural crops at the present study. Tilapia fries with 6.2 g and 5.9 g mean weight and stocking density of 40 m were respectively stocked in two 2.5 m tanks at September 2013 for 9 months. Fish culture in the two tanks tended to 17.2 kg/m and 19 kg/m fish production with 658 g and 596 mean final weights, 98% and 100% survival and 1.4 and 1.6 food conversion rates. Plants were implanted using grow beds, floating rafts and nutrient film methods. Monthly production of mint, basil, lettuce, cucumber, tomato and pepper were 1.14, 0.5-0.8, 1.32, 2.7-4, 2.03-3.4 and 1.1 kg/m crops, respectively.
缺水和农业土壤不足是限制农学发展的因素,特别是在伊朗等干旱地区。罗非鱼因其生长快、对环境条件和疾病的耐受性、集约化养殖的可能性、低生产成本和适销对路的风味而成为重要的商业鱼类。在水培系统中生产水生和农业作物将满足这些期望。研究了水培系统和循环水系统相结合的水培系统在罗非鱼和农作物调节养殖中的应用。2013年9月,将平均体重为6.2 g和5.9 g、放养密度为40 m的罗非鱼苗分别放养在2个2.5 m的鱼缸中,放养9个月。两个鱼缸的鱼产量分别为17.2 kg/m和19 kg/m,平均末重分别为658 g和596 g,成活率分别为98%和100%,食物转化率分别为1.4和1.6。采用种植床、浮筏和营养膜三种方法种植植物。薄荷、罗勒、生菜、黄瓜、番茄和辣椒的月产量分别为1.14、0.5 ~ 0.8、1.32、2.7 ~ 4、2.03 ~ 3.4和1.1 kg/m。
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引用次数: 3
期刊
Modern Agricultural Science and Technology
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