Determination of taxifolin in functional food samples by high - performance liquid chromatography (HPLC - UV Vis)

Abstract

A method using high performance liquid chromatography with UV - Vis detector was developed for quantification of taxifolin in supplements. In this study, the supplements were homogenized, extracted by ultrasonication with methanol. Taxifolin in test solution was separated on C18 column (250 × 4.6 mm, 5 µm) with mobile phase containing methanol and 0.3 % acetic acid solution (40 : 60) using isocratic elution at a flow rate of 1.0 mL/min, with UV detection at 290 nm. The analytical method was validated for specificity, linearity, precision and accuracy according to AOAC. The detector response for taxifolin was linear over the selected concentration range from 1.04 to 78.23 µg/mL with a correlation coefficient 0.9996. The repeatability (RSD, n = 7) were from 1.1 to 1.4 % respectively. The recovery was between 98.1 and 105.9 %. The method was successfully applied for analysis of taxifolin in 7 supplements at solid, liquid and oil form.