EFFECTS OF MAGNETIC-ACTIVATED SPERM CELL SORTING TECHNIQUE IN BLASTOCYST FORMATION AFTER INTRACYTOPLASMIC SPERM INJECTION

Abstract

Many sperm preparation procedures are used in intracytoplasmic sperm injection (ICSI), including migration, density gradient centrifugation, and filtering. Magnetic-activated cell sorting (MACS) is a type of magnetic separation that has been used in a variety of biomedical and biological studies. To examine the fertilisation rate, cleavage, and development of Blastocystes in two sperm preparation procedures for ICSI, MACS and density gradient centrifugation (DGC). The research was carried out in the Royal Center in Mansoura, Dakahlia Governorate, Egypt. Fifty patients receiving ICSI had their sperm samples taken. The sperm were separated into two groups using traditional DGC. One group served as DGC, while the other served as MACS. The number of oocytes harvested, the number of metaphase II oocytes injected, fertilisation rates, cleavage rates, and embryo quality were all compared. between MACS and groups. quality, statistically significant difference between the two groups in terms of the of quality and of MII a statistically significant negative connection. in the DGC group, a statistically significant negative connection between sperm DNA fragmentation (SDF) and fertilisation rate. MACS sperm selection of human spermatozoa is a safe, straightforward, and effective procedure for sperm preparation in a clinical environment for ICSI..