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Association of IL-6 rs1800795 and IL-1 β rs16944 polymorphisms with non-small cell lung cancer in the Egyptian population: a pilot study 埃及人群中 IL-6 rs1800795 和 IL-1 β rs16944 多态性与非小细胞肺癌的关系:一项试点研究
Pub Date : 2023-12-10 DOI: 10.21608/sjdfs.2023.248290.1144
Yomna F Metwally, Rasha F Zahran, Rana R Elsadda, S. Refaat, Afaf M. Elsaid
Lung cancer is a serious health and life issue, with the highest rates of incidence and mortality in the world. It is now clear that inflammation is a key factor involved in all aspects of carcinogenesis, notably lung cancer development. Genetic changes, including polymorphisms in inflammatory genes, are supposed to play a significant role in increasing lung cancer risk. In this study, we aim to investigate the association of IL-6 rs1800795 and IL-1β rs16944 polymorphisms with non-small cell lung cancer (NSCLC) development in the Egyptian population. The study design was composed of 100 NSCLC cases and 100 controls, which were genotyped using the ARMS-PCR technique, electrophoresed on a 2.5% agarose gel, and visualized using ethidium bromide under ultraviolet illumination. The IL-1β rs16944 genotypes were significantly different in NSCLC patients as compared to healthy controls ( p = 0.032). Whereas the genotypes and alleles of the IL-6 rs1800795 were not significantly linked to NSCLC incidence ( p = 0.726; p = 0.822, respectively). To our best knowledge, this study aimed to uncover the great impact of IL-1β rs16944 genotypes on NSCLC development in the Egyptian population; thus, it may be a gateway for earlier NSCLC prevention.
肺癌是一个严重的健康和生命问题,是世界上发病率和死亡率最高的疾病。目前已明确的是,炎症是参与各方面致癌过程的关键因素,尤其是肺癌的发展。基因变化,包括炎症基因的多态性,被认为在增加肺癌风险方面起着重要作用。本研究旨在调查埃及人群中 IL-6 rs1800795 和 IL-1β rs16944 多态性与非小细胞肺癌(NSCLC)发病的关系。研究设计了 100 例 NSCLC 病例和 100 例对照,采用 ARMS-PCR 技术进行基因分型,在 2.5% 琼脂糖凝胶上电泳,并在紫外光下用溴化乙锭显像。与健康对照组相比,NSCLC 患者的 IL-1β rs16944 基因型有显著差异(p = 0.032)。而IL-6 rs1800795的基因型和等位基因与NSCLC发病率无明显关系(分别为p = 0.726和p = 0.822)。据我们所知,这项研究旨在揭示IL-1β rs16944基因型对埃及人群NSCLC发病的巨大影响,因此,它可能是早期预防NSCLC的一个途径。
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引用次数: 0
Electrochemical sensing of antipyrine and its interaction with ds-DNA modified electrode. 安替比林的电化学传感及其与 ds-DNA 修饰电极的相互作用。
Pub Date : 2023-12-10 DOI: 10.21608/sjdfs.2023.221623.1116
ahmed ramadan, abdelgawad radi
The interaction of antipyrine (AnP) with the double-stranded deoxyribonucleic acid of salmon sperm on a electrochemical electrode (GCE) was carefully examined using voltammetric methods.The Ultra violet-visible absorption method was used to determine the interaction of AnP and double strand DNA in solution. AnP give a single anodic peak in buffer solutions of (pH 3.5 - 9.5).AnP is oxidized in a diffusion-controlled process from a phosphate buffer solution, pH 7.The variation given by AnP through its current signals was monitored in the existance of DNA by GCE using different voltammetric techniques. Diffusion coefficient of free AnP was 3.7×10 -6 and 2.8×10 -7 cm 2 s -1 for the AnP-DNA .The binding constant was calculated to be 1.5×10 5 and 1.1×10 5 M -1 using DPV and UV / vis Spectroscopy. AnP binds to DNA primarily by electrostatic interaction with a contribution from intercalation. A detection pattern depends on pre-concentration and DPV detection at GCE modified with dsDNA was introduced to determine AnP in saliva matrices.
采用伏安法仔细研究了安替比林(AnP)与电化学电极(GCE)上鲑鱼精子双链脱氧核糖核酸的相互作用。在 pH 值为 3.5 - 9.5 的缓冲溶液中,AnP 会产生一个阳极峰。AnP 在 pH 值为 7 的磷酸盐缓冲溶液中通过扩散控制过程被氧化。使用 DPV 和 UV / vis 光谱法计算出的结合常数分别为 1.5×10 5 和 1.1×10 5 M -1 。AnP 与 DNA 的结合主要是通过静电作用,也有插层作用。唾液基质中 AnP 的检测模式取决于预浓缩和在用 dsDNA 修饰的 GCE 上进行 DPV 检测。
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引用次数: 0
Morphological and Ultrastructural Changes of Escherichia coli and Klebsiella pneumoniae carriers of β-lactamase when subject to β-lactam Antibiotic 大肠埃希菌和肺炎克雷伯菌在β-内酰胺抗生素作用下的β-内酰胺酶携带者形态学和超微结构变化
Pub Date : 2023-12-09 DOI: 10.21608/sjdfs.2023.230321.1127
yahya bdreldien, Mohamed Abou-Dobara, A. El-Sayed, Mohamed Zaghloul
In our manuscript, we examine the structure and morphology, we take an imperative look at this pathway. We collected clinical isolates from patients at the Oncology Center of Mansoura University, from urine samples. The isolates were then screened for antibiotic resistance and beta-lactamase enzyme production, with a focus on the most prevalent bacterial isolates. To further investigate the impact of one particular beta-lactam antibiotic on Escherichia coli and Klebsiella pneumoniae , both known producers of the beta-lactamase enzyme, we utilized Transmission Electron and Scanning Electron Microscopes. Our study aims to discuss the various changes induced by cefoxitin, including its effects on the characteristics of clinical isolates such as the cell wall structure of E. coli and K. pneumoniae . The aim of this manuscript is to discuss the occurrence of alterations caused by beta-lactam antibiotics. Photomicrographs will be used to describe these alterations and provide information about the agents responsible for the changes. We will focus on the structural and morphological changes that are consistently observed.
在我们的手稿中,我们对其结构和形态进行了研究,并对这一途径进行了深入探讨。我们从曼苏尔大学肿瘤中心患者的尿液样本中收集了临床分离菌株。然后对分离物进行了抗生素耐药性和β-内酰胺酶生产筛选,重点是最常见的细菌分离物。为了进一步研究一种特殊的β-内酰胺类抗生素对大肠埃希菌和肺炎克雷伯菌的影响,我们使用了透射电子显微镜和扫描电子显微镜。我们的研究旨在讨论头孢西丁引起的各种变化,包括其对临床分离菌特性的影响,如大肠杆菌和肺炎克雷伯菌的细胞壁结构。本手稿旨在讨论β-内酰胺类抗生素引起的变化。我们将使用显微照片来描述这些变化,并提供导致这些变化的药剂的相关信息。我们将重点关注持续观察到的结构和形态变化。
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引用次数: 0
Effect of phosphorus form and culturing mode on mycelial growth of Pleurotus pulmonarius and Pleurotus floridanus on rice straw 磷的形态和培养模式对稻草上的褶菌和花褶菌菌丝生长的影响
Pub Date : 2023-12-09 DOI: 10.21608/sjdfs.2023.222643.1118
Amira El-Fallal, T. El-Katony, Heba Dahap, H. El-Gharabawy
Oyster mushroom ( Pleurotus spp.) cultivation gained a growing concern due to their limited growth requirements and high nutritional value and bioactive compounds. Present work investigates the effect of rice straw supplementation with inorganic P (KH 2 PO 4 ) and organic P (wheat bran) with presence or absence of agar layer above straw on mycelial growth of two oyster mushrooms: P. pulmonarius and P. floridanus. Mycelial growth was better with bran than with KH 2 PO 4 , particularly without agar. Such a differential effect of P form and agar layer was more evident on P. pulmonarius than on P. floridanus . Mycelial growth mostly exhibited a saturable dose-dependent pattern and increased up to a certain optimal P supply of 2–5 g P/g straw, post which it approached a plateau; only in the no agar-bran culture of P. floridanus a progressive P-dependent increase was found. The time-course of fungal growth exhibited contrasting patterns in the two fungi; being sigmoidal in P. pulmonarius but almost linear in P. floridanus . In P. pulmonarius , the lag growth period (T 10 ) was longer with KH 2 PO 4 than with bran, with different dose-dependent patterns in the two P sources and marginal effect of the agar layer. Relative growth rate (RGR) of P. pulmonarius was higher than that of P. floridanus , with different dose-dependent patterns according to fungal species and presence of agar. In P. pulmonarius , both T 10 and RGR attained comparable values of about one day and 0.4 day -1 , respectively at 5 mg P/g straw for the different P form × culturing mode combinations..
由于生长需求有限、营养价值高且含有生物活性化合物,杏鲍菇的种植越来越受到关注。本研究调查了稻草添加无机磷(KH 2 PO 4)和有机磷(麦麸)以及稻草上是否有琼脂层对两种杏鲍菇菌丝生长的影响:P. pulmonarius 和 P. floridanus。使用麦麸比使用 KH 2 PO 4,尤其是不使用琼脂时,菌丝生长更好。P 形态和琼脂层对 P. pulmonarius 的不同影响比对 P. floridanus 更明显。菌丝生长大多表现出饱和剂量依赖模式,并在达到 2-5 克 P/g 稻草的最佳 P 供给量后开始增长,之后趋于平稳;只有在无琼脂麸皮的花叶豚草培养物中发现了逐渐增长的 P 依赖性。真菌生长的时间过程在两种真菌中表现出截然不同的模式;在 P. pulmonarius 中是曲线生长,而在 P. floridanus 中几乎是直线生长。在肺真菌中,KH 2 PO 4 的滞后生长期(T 10 )比麸皮的滞后生长期(T 10 )长,两种 P 源的剂量依赖模式不同,琼脂层的影响微乎其微。P. pulmonarius 的相对生长率(RGR)高于 P. floridanus,真菌种类和琼脂存在不同的剂量依赖模式。在 5 mg P/g 稻草条件下,对于不同的 P 形态 × 培养模式组合,P. pulmonarius 的 T 10 和 RGR 分别达到了约 1 天和 0.4 天 -1 的可比值。
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引用次数: 0
Effect of Temperature on Biological Aspects, Fecundity and Life Table Parameters of The Predatory Mite, Cheyletus malaccensis 温度对食肉螨(Cheyletus malaccensis)的生物学特性、繁殖力和生命表参数的影响
Pub Date : 2023-12-09 DOI: 10.21608/sjdfs.2023.209617.1107
zeinab Abdelsalam, H. Taha, mohamed elbokl, Essam Yasin
The predatory mite, Cheyletus malaccensis (Oud.) was recorded to be the communist predacious mites of family Cheyletidae, it was found associated with other different mite species and immature stages of insects, which were collected from palm tree fibers in Damietta Governorate. Biological aspects, fecundity and life table parameters of Cheyletus malaccensis were studied under laboratory conditions at 25 and 35 o C and 65% R.H. Feeding on Immatures of Tyrophagus putrescentiae . Obtained results showed that the developmental stages of both female and male were affected by temperature as a vital environmental factor, were as its decreased as well as temperature increased. Also, deposited an average of 98.9 and 57.75 eggs during 54.18 and 13.1 days, with a daily rate of 1.8 and 4.5 eggs at 25 and 35 o C. Life table parameters showed highest value of the net reproductive rate (R o ) as 62.83 at 25 o C. Sex ratios were 0.46 for both temperatures.
捕食螨 Cheyletus malaccensis (Oud.) 被记录为 Cheyletidae 科的共生捕食螨,它与其他不同种类的螨类和昆虫的未成熟阶段伴生,这些昆虫是从达米埃塔省的棕榈树纤维中采集的。在 25 和 35 o C 及 65% R.H. 的实验室条件下研究了 Malaccensis Cheyletus 的生物学方面、繁殖力和生命表参数。研究结果表明,雌虫和雄虫的发育阶段都受到温度这一重要环境因素的影响,温度越低,雌虫和雄虫的发育阶段就越长。生命表参数显示,25℃时的净生殖率(R o)最高,为 62.83。
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引用次数: 0
Oral intake of Pomegranate Peels Stimulate Glutathione levels and Superoxide Dismutase Activity to Protect Against Cisplatin-Induced Nephrotoxicity 口服石榴皮可提高谷胱甘肽水平和超氧化物歧化酶活性,从而防止顺铂诱发的肾毒性
Pub Date : 2023-12-09 DOI: 10.21608/sjdfs.2023.230342.1128
E. Toson, Nagwa M. Abdelhadi, Abdel-Aziz F. Abdel-Aziz
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引用次数: 0
Phenotypic plasticity of four invasive species in north-eastern section of the Nile Delta coast, Egypt 埃及尼罗河三角洲东北部海岸四种入侵物种的表型可塑性
Pub Date : 2023-12-09 DOI: 10.21608/sjdfs.2023.223602.1120
M. Serag, Reham elbarougy, Hanan Abo Elagras, Abdel-Hamid Khedr
One of the essential mechanisms through which plants can adapt to prevailing environmental conditions is phenotypic plasticity. To explain the invasive success across the invaded new areas, the phenological pattern and vegetative features of the exotic species are investigated. The four exotic weeds included in this study are Xanthium strumarium L., Bassia indica (Wight) A. J. Scott, Atriplex lindleyi Moq., and Trianthema portulacastrum L. Results indicated that shoot length was greatest in X. strumarium, followed by B. indica and A. lindleyi but least in T. portuiaeustrum. Fresh weight of the foliage was comparable in X. strumarium and A. lindleyi, being higher in both than in B. indica and T. portulacastrum. Dry wight of the foliage was highest in X. strumarium, followed by A. Lindleyi, but it was least with comparable values of B. indica and T. portulacastrum. Water content of shoots was higher in T. portulacastrum and B. indica, slightly lower in X. strumarium and least in A. lindleyi. The highest concentrations of P (0.620.02), K (0.060.001), and Mg (24.763.79) were found in site 2 of T. portulacastrum. The highest levels of N (6.070.30), Na (0.200.009), and moisture content (44.320.40) were found in site 3 for B. indica. The components with the lowest concentrations were P (0.100.01), K(0.010.0003), Mg (0.370.01), and Na (0.040.003) in A. Lindleyi at site 4, whereas N (2.171.00) and moisture content (17.215.10) were the lowest in X. strumarium at site 1.Analysis of variance revealed significant (P<0.05) to very highly significant (P<0.001) variation in growth parameter among the studied plant species. Based on the magnitude of the F ratio, the variability was most evident in shoot dry weight, followed by shoot fresh weight and water content but was relatively low in number of leaves, number of branches and water content of shoot. Based on the obtained results, it can be concluded that the four exotic weeds can successfully invade new areas by changing their phenological plasticity. The optimal management and control measures of these exotic weeds will benefit from this .
表型可塑性是植物适应当前环境条件的重要机制之一。为了解释外来物种成功入侵新地区的原因,本研究对外来物种的表型和植株特征进行了调查。结果表明,X. strumarium 的嫩枝长度最大,其次是 B. indica 和 A. lindleyi,而 T. portuiaeustrum 的嫩枝长度最小。X. strumarium 和 A. lindleyi 的叶片鲜重相当,都高于 B. indica 和 T. portulacastrum。叶片的干重在 X. strumarium 中最高,其次是 A. Lindleyi,但在 B. indica 和 T. portulacastrum 中最低。T. portulacastrum 和 B. indica 的嫩枝含水量较高,X. strumarium 稍低,A. lindleyi 最低。在 T. portulacastrum 的第 2 个地点,P(0.620.02)、K(0.060.001)和 Mg(24.763.79)的浓度最高。在第 3 个地点,B. indica 的 N(6.070.30)、Na(0.200.009)和水分含量(44.320.40)含量最高。方差分析显示,所研究的植物物种之间的生长参数存在显著(P<0.05)到非常显著(P<0.001)的差异。根据 F 比的大小,差异最明显的是嫩枝干重,其次是嫩枝鲜重和含水量,但叶片数、分枝数和嫩枝含水量的差异相对较小。根据所得结果,可以得出结论:这四种外来杂草可以通过改变其物候可塑性成功入侵新的地区。对这些外来杂草的优化管理和控制措施将从中受益。
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引用次数: 0
On the generation of ion-acoustic super-periodic waves in ultra-relativistic degenerate quantum plasma 论超相对论退化量子等离子体中离子声超周期波的产生
Pub Date : 2023-12-09 DOI: 10.21608/sjdfs.2023.210193.1108
E. Habbak, M. Selim, S. El-Labany
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引用次数: 0
A simple point score model for prediction of Covid-19 in some Egyptian patients 预测部分埃及患者 Covid-19 的简单点分模型
Pub Date : 2023-12-09 DOI: 10.21608/sjdfs.2023.208364.1105
Reham el fanagely, Ayman Hyder, Hekmat Elgammal, Atef Wahdn
On a global scale, a substantial number of newly diagnosed cases of the covid-19 virus and a considerable number of associated fatalities are recorded weekly. Its laboratory detection depends on the costly and time-consuming real-time PCR analysis. A simple way to facilitate the diagnosis of Covid-19 is still required. Here, it was aimed to generate a simple point score as a prediction model for fast diagnosis of Covid-19 using simple laboratory analyses. 121 adult individuals with qRT-PCR results served as a training group, whereas 35 individuals were used as a validation group. Different laboratory analyses, including complete blood count (CBC), differential count, D-dimer, C-Reactive Protein (CRP), and Ferritin, have been recruited as predictors using the Receiver Operating Characteristic (ROC) analysis. The results revealed three models, depending on the predictor parameters' ROC area (AUC). The simplest model consisted of the data of the three predictors: lymphocytopenia, CRP, and D-dimer, and resulted in a ROC AUC value of 0.9773. The use of the three models on the validation group provided support for the conclusion that the calculation of lymphocyte count, CRP, and D-dimer is enough for predicting the occurrence of Covid-19.
在全球范围内,每周都有大量新确诊的科维-19 病毒病例和相当数量的相关死亡病例。其实验室检测依赖于昂贵而耗时的实时 PCR 分析。目前仍需要一种简单的方法来帮助诊断 Covid-19。在此,我们旨在通过简单的实验室分析,生成一个简单的点评分,作为快速诊断 Covid-19 的预测模型。121名有qRT-PCR结果的成人作为训练组,35名作为验证组。使用接收方操作特征(ROC)分析法,将不同的实验室分析,包括全血细胞计数(CBC)、差值计数、D-二聚体、C-反应蛋白(CRP)和铁蛋白作为预测指标。结果显示,根据预测参数的 ROC 区域(AUC),有三种模型。最简单的模型由淋巴细胞减少、CRP 和 D-二聚体这三个预测参数的数据组成,其 ROC AUC 值为 0.9773。在验证组中使用这三个模型为以下结论提供了支持,即计算淋巴细胞计数、CRP 和 D-二聚体足以预测 Covid-19 的发生。
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引用次数: 0
Antibacterial activity of optimized extracellular biosynthesized zinc oxide nanoparticles using Corynebacterium sp. ATCC 6931 优化的细胞外生物合成氧化锌纳米粒子的抗菌活性(以金杆菌 ATCC 6931 为研究对象
Pub Date : 2023-12-09 DOI: 10.21608/sjdfs.2023.231788.1129
Amira Abd El-Nour, Mohamed Abou-Dobara, A. El-Sayed, M. El-Zahed
Nanoparticle (NP) green synthesis is gaining popularity and has been proposed as a potential substitute for chemical and physical processes. The current study reports the environmentally friendly, low-cost method of optimized zinc oxide nanoparticles (ZnO NPs) utilizing a crude metabolite from Corynebacterium sp. ATCC 6931. Aliquot pH 8, 35°C, and an 8:2 (v/v) ratio of bacterial supernatant to zinc nitrate solution were the best conditions for the formation of ZnO NPs. Transmission electron microscopy (TEM), UV-visible spectroscopy (UV-vis), X-ray diffraction (XRD), and Zeta potential analyses were used to characterize the biosynthesized NPs. The synthesized ZnO NPs had sizes ranging from 8 to 17 ± 1.23 nm were mainly spherical in form and had a positive charge of ≈ +18.9 mV. The biosynthesized ZnO NPs in the current study have been applied in antimicrobial applications. Using the agar well diffusion method, different concentrations of biosynthesized ZnO NPs (50 µg/mL, 100 µg/mL, and 150 µg/mL) were used to test the antibacterial activity of the characterized NPs against the Gram-positive and Gram-negative bacteria: Bacillus cereus, Corynebacterium sp., and Escherichia coli. Additionally, the minimum inhibitory concentration (MIC) test was applied, and the results showed that bacterial growth reduces as biosynthesized ZnO NPs concentration increases. Also, compared to Gram-positive bacteria, Gram-negative bacteria appeared to be more sensitive to ZnO NPs.
纳米粒子(NP)绿色合成越来越受欢迎,并被认为是化学和物理过程的潜在替代品。本研究报告了一种环境友好型、低成本的优化氧化锌纳米粒子(ZnO NPs)的方法,该方法利用的是一种来自 Corynebacterium sp. ATCC 6931 的粗代谢产物。等体积 pH 值为 8、温度为 35°C、细菌上清液与硝酸锌溶液的比例为 8:2(v/v)是形成 ZnO NPs 的最佳条件。透射电子显微镜(TEM)、紫外-可见光谱(UV-vis)、X 射线衍射(XRD)和 Zeta 电位分析被用来表征生物合成的 NPs。合成的 ZnO NPs 大小为 8 至 17 ± 1.23 nm,主要呈球形,带正电荷 ≈ +18.9 mV。本研究中生物合成的 ZnO NPs 已应用于抗菌领域。利用琼脂井扩散法,使用不同浓度的生物合成 ZnO NPs(50 微克/毫升、100 微克/毫升和 150 微克/毫升)来测试其对革兰氏阳性和革兰氏阴性细菌的抗菌活性:蜡样芽孢杆菌、棒状杆菌和大肠杆菌。结果表明,随着生物合成 ZnO NPs 浓度的增加,细菌的生长速度减慢。此外,与革兰氏阳性菌相比,革兰氏阴性菌似乎对氧化锌氮氧化物更敏感。
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引用次数: 0
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Scientific Journal for Damietta Faculty of Science
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