Callogenesis of Durio zibethinus using flower bud explant

Y. Anggraito, N. Hermayani, M. Abdullah, N. A. Habibah, A. Retnoningsih
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引用次数: 1

Abstract

Propagation of superior durian (Durio zibethinus) are constrained due to the limited number of the tree. Grafting and budding are commonly used in durian propagation, however these techniques give destructive effect to mother plant. Alternatively, in vitro technique can be chosen for propagation of superior durian. The research aims to study the possibility of durian flower bud as an explant for micropropagation because this part is relatively more sterile than other organs. Half strength Murashige dan Skoog media were used to grow explant with addition of 1 ppm 2,4-dichlorophenoxyacetic acid (2,4-D) and various combinations of sucrose concentration (30 gr/L, 50 gr/L and 70 gr/L), and Thidiazuron (TDZ, 0.2 ppm, 0.4 ppm and 0.8 ppm). Totally, there were nine treatment combinations with six replications, respectively. Parameters observed were number of explants-producing callus, colour and callus morphology. The result indicated that flower bud of durian had a potential as an explant for micropropagation due to their ability to induce callogenesis (69.2%). Combination of 45 gr/L sucrose and 0.8 ppm TDZ gave the best result in inducing callus growth. Most of the callus showed white colour and friable callus.Propagation of superior durian (Durio zibethinus) are constrained due to the limited number of the tree. Grafting and budding are commonly used in durian propagation, however these techniques give destructive effect to mother plant. Alternatively, in vitro technique can be chosen for propagation of superior durian. The research aims to study the possibility of durian flower bud as an explant for micropropagation because this part is relatively more sterile than other organs. Half strength Murashige dan Skoog media were used to grow explant with addition of 1 ppm 2,4-dichlorophenoxyacetic acid (2,4-D) and various combinations of sucrose concentration (30 gr/L, 50 gr/L and 70 gr/L), and Thidiazuron (TDZ, 0.2 ppm, 0.4 ppm and 0.8 ppm). Totally, there were nine treatment combinations with six replications, respectively. Parameters observed were number of explants-producing callus, colour and callus morphology. The result indicated that flower bud of durian had a potential as an explant for micropropagation du...
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利用花芽外植体研究紫堇的胼胝质形成
优质榴莲(Durio zibethinus)的繁殖受到数量限制。嫁接和出芽是榴莲常用的繁殖技术,但这些技术对母株有破坏性影响。或者,可以选择体外技术进行优质榴莲的繁殖。由于榴莲花蕾部分相对其他器官不育性较差,本研究旨在研究将其作为外植体进行微繁的可能性。采用半强度Murashige dan Skoog培养基,在1 ppm 2,4-二氯苯氧乙酸(2,4- d)和蔗糖浓度(30、50、70 g /L)、噻脲(TDZ、0.2、0.4、0.8 ppm)的组合下进行外植体生长。共9个治疗组合,分别6个重复。观察外植体愈伤组织的数量、颜色和形态。结果表明,榴莲花芽具有诱导愈伤组织发生的能力(69.2%),具有作为外植体进行微繁的潜力。45 g /L蔗糖和0.8 ppm TDZ组合对愈伤组织的诱导效果最好。大部分愈伤组织呈白色,且愈伤组织易碎。优质榴莲(Durio zibethinus)的繁殖受到数量限制。嫁接和出芽是榴莲常用的繁殖技术,但这些技术对母株有破坏性影响。或者,可以选择体外技术进行优质榴莲的繁殖。由于榴莲花蕾部分相对其他器官不育性较差,本研究旨在研究将其作为外植体进行微繁的可能性。采用半强度Murashige dan Skoog培养基,在1 ppm 2,4-二氯苯氧乙酸(2,4- d)和蔗糖浓度(30、50、70 g /L)、噻脲(TDZ、0.2、0.4、0.8 ppm)的组合下进行外植体生长。共9个治疗组合,分别6个重复。观察外植体愈伤组织的数量、颜色和形态。结果表明,榴莲花芽具有作为微繁殖外植体的潜力。
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