Isobutyl Isothiocyanate is a Potent Heat Tolerance Enhancer for Arabidopsis

Q3 Agricultural and Biological Sciences Environmental Control in Biology Pub Date : 2018-01-01 DOI:10.2525/ECB.56.121
M. Hara, Takumi Matsubara, I. Takahashi, Hiroki Murano
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引用次数: 2

Abstract

Heat tolerance enhancers are chemicals which prevent the physiological damages of plants by heat. Phenethyl isothiocyanate (ITC), which is known as the heat tolerance enhancer, induced the expression of heat shock protein genes in Arabidopsis . Here we measured the heat shock response (HSR)-inducing activities of ITCs to find more active ITCs than phenethyl ITC. We prepared transgenic Arabidopsis possessing the b - glucuronidase reporter gene driven by the promoter of a small heat shock protein ( HSP17.6C-CI ) gene which was induced by heat tolerance enhancers. We tested 16 naturally occurring ITCs for their HSR-inducing activities: 5 aromatic ITCs, 7 aliphatic ITCs, and 4 sulfur-containing aliphatic ITCs. The results showed that the short-chain aliphatic ITCs, especially isobutyl ITC, promoted the HSR more strongly than the aromatic and sulfur-containing aliphatic ITCs. Isobutyl ITC induced the HSR in the whole Arabidopsis seedlings. The isobutyl ITC-treated plants showed higher heat tolerance than the phenethyl ITC-treated plants. These results suggest that isobutyl ITC is a more potent heat tolerance enhancer than phenethyl ITC.
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异硫氰酸异丁酯是一种有效的拟南芥耐热性增强剂
耐热增强剂是一种防止植物受热生理损害的化学物质。被称为耐热增强剂的异硫氰酸苯乙酯(ITC)诱导了拟南芥热休克蛋白基因的表达。在这里,我们测量了ITCs诱导热休克反应(HSR)的活性,发现ITCs比苯基ITC更活跃。我们制备了含有b -葡萄糖醛酸酶报告基因的转基因拟南芥,该基因由耐热增强剂诱导的小热休克蛋白(HSP17.6C-CI)基因启动子驱动。我们测试了16种天然存在的ITCs的hsr诱导活性:5种芳香ITCs, 7种脂肪ITCs和4种含硫脂肪ITCs。结果表明,短链脂肪族ITC比芳香型和含硫型脂肪族ITC对HSR的促进作用更强,尤其是异丁基ITC。异丁基ITC诱导拟南芥全苗HSR。异丁基icc处理植株的耐热性高于苯乙基icc处理植株。这些结果表明异丁基ITC是一种比苯基ITC更有效的耐热性增强剂。
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来源期刊
Environmental Control in Biology
Environmental Control in Biology Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
2.00
自引率
0.00%
发文量
25
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