High-Voltage Electric Discharge Extraction of Bioactive Compounds from the Cocoa Bean Shell

IF 1.6 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Chemical and Biochemical Engineering Quarterly Pub Date : 2019-03-01 DOI:10.15255/CABEQ.2018.1525

S. Jokić, N. Pavlović, A. Jozinović, Đ. Ačkar, J. Babić, D. Šubarić

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引用次数: 14

Abstract

This study is focused on the application of high-voltage electric discharge (HVED) to recover some bioactive compounds from the cocoa bean shell. Different extraction times (30, 60, 90 min), frequencies (40, 70, 100 Hz) and solvent-solid ratios (10, 30 and 50 mL g–1) were used to obtain cocoa bean shell extracts. Desired bioactive compounds, methylxanthines and phenolic compounds were measured in obtained extracts by high-pressure liquid chromatography with diode array detector. The obtained extracts showed that theobromine was the most abundant, ranging from 2530.13 to 6031.51 mg kg–1, while caffeine content was in the range from 316.08 to 849.88 mg kg–1. In addition, significant amounts of phenolic compounds were found, namely catechin (115.91 to 284.33 mg kg–1), epicatechin (20.20 to 358.90 mg kg–1), and gallic acid (80.28 to 219.17 mg kg–1). Results showed that different parameters of HVED extraction have statistically significant influence on cocoa bean shell composition, suggesting how this byproduct can be used in the production of valuable extracts.

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高压放电提取可可豆壳中生物活性物质的研究

本文研究了利用高压放电技术从可可豆壳中回收一些生物活性化合物。采用不同的提取时间(30、60、90 min)、频率(40、70、100 Hz)和液固比(10、30、50 mL g-1)得到可可豆壳提取物。用高压液相色谱二极管阵列检测器检测提取液中所需的生物活性物质、甲基黄嘌呤和酚类化合物。所得提取物中可可碱含量最高，为2530.13 ~ 6031.51 mg kg-1，咖啡因含量为316.08 ~ 849.88 mg kg-1。此外，还发现了大量的酚类化合物，即儿茶素(115.91至284.33 mg kg-1)、表儿茶素(20.20至358.90 mg kg-1)和没食子酸(80.28至219.17 mg kg-1)。结果表明，不同的HVED提取参数对可可豆壳成分的影响具有统计学意义，这表明该副产品可以用于生产有价值的提取物。

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来源期刊

Chemical and Biochemical Engineering Quarterly 工程技术-工程：化工

CiteScore

2.70

自引率

6.70%

发文量

审稿时长

>12 weeks

期刊介绍： The journal provides an international forum for presentation of original papers, reviews and discussions on the latest developments in chemical and biochemical engineering. The scope of the journal is wide and no limitation except relevance to chemical and biochemical engineering is required. The criteria for the acceptance of papers are originality, quality of work and clarity of style. All papers are subject to reviewing by at least two international experts (blind peer review). The language of the journal is English. Final versions of the manuscripts are subject to metric (SI units and IUPAC recommendations) and English language reviewing. Editor and Editorial board make the final decision about acceptance of a manuscript. Page charges are excluded.